The lattice-like structure observed by Vipp1-GFP in arabidopsis chloroplasts

Lingang Zhang, Yusuke Kato, Koji Saigo, Ute C. Vothknecht, Wataru Sakamoto

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Vipp1 (vesicle inducing protein in plastids 1) is proposed to play a role in thylakoid biogenesis. It is closely related to PspA (phage shock protein A), a bacterial protein that is induced under stress conditions. Despite its discovery a decade ago and extensive analysis in cyanobacteria, green algae and higher plants, the precise role of Vipp1 in the process of chloroplast development remains unclear. In this research, we expressed Vipp1 C-terminally fused to GFP (Vipp1-GFP) in Arabidopsis and found that Vipp1 is able to assemble into rod-shaped supercomplexes. Vipp1-GFP can rescue heterotrophic growth of a vipp1 knock-down mutant, suggesting that it complements Vipp1 function. Interestingly, Vipp1-GFP rods always appeared to cross with each other to form a lattice-like structure, which is similar to a scaffold structure formed by PspA in Escherichia coli. Based on these results, we infer that Vipp1 is involved in not only thylakoid biogenesis but chloroplast envelope integrity.

Original languageEnglish
Title of host publicationAdvanced Topics in Science and Technology in China
PublisherSpringer
Pages394-397
Number of pages4
DOIs
Publication statusPublished - Jan 1 2013

Publication series

NameAdvanced Topics in Science and Technology in China
ISSN (Print)1995-6819
ISSN (Electronic)1995-6827

Fingerprint

Proteins
Bacteriophages
Staphylococcal Protein A
Bacterial Proteins
Algae
Scaffolds
Escherichia coli
Scaffolds (biology)

Keywords

  • Arabidopsis
  • Chloroplast
  • Lattice-like structure
  • PspA
  • Vipp1

ASJC Scopus subject areas

  • Engineering(all)
  • Chemical Engineering(all)
  • General

Cite this

Zhang, L., Kato, Y., Saigo, K., Vothknecht, U. C., & Sakamoto, W. (2013). The lattice-like structure observed by Vipp1-GFP in arabidopsis chloroplasts. In Advanced Topics in Science and Technology in China (pp. 394-397). (Advanced Topics in Science and Technology in China). Springer. https://doi.org/10.1007/978-3-642-32034-7_82

The lattice-like structure observed by Vipp1-GFP in arabidopsis chloroplasts. / Zhang, Lingang; Kato, Yusuke; Saigo, Koji; Vothknecht, Ute C.; Sakamoto, Wataru.

Advanced Topics in Science and Technology in China. Springer, 2013. p. 394-397 (Advanced Topics in Science and Technology in China).

Research output: Chapter in Book/Report/Conference proceedingChapter

Zhang, L, Kato, Y, Saigo, K, Vothknecht, UC & Sakamoto, W 2013, The lattice-like structure observed by Vipp1-GFP in arabidopsis chloroplasts. in Advanced Topics in Science and Technology in China. Advanced Topics in Science and Technology in China, Springer, pp. 394-397. https://doi.org/10.1007/978-3-642-32034-7_82
Zhang L, Kato Y, Saigo K, Vothknecht UC, Sakamoto W. The lattice-like structure observed by Vipp1-GFP in arabidopsis chloroplasts. In Advanced Topics in Science and Technology in China. Springer. 2013. p. 394-397. (Advanced Topics in Science and Technology in China). https://doi.org/10.1007/978-3-642-32034-7_82
Zhang, Lingang ; Kato, Yusuke ; Saigo, Koji ; Vothknecht, Ute C. ; Sakamoto, Wataru. / The lattice-like structure observed by Vipp1-GFP in arabidopsis chloroplasts. Advanced Topics in Science and Technology in China. Springer, 2013. pp. 394-397 (Advanced Topics in Science and Technology in China).
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