The Langerhans cell granule is an adsorptive endocytic organelle

M. Takigawa, K. Iwatsuki, M. Yamada, H. Okamoto, S. Imamura

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

The role of Langerhans cell granules (LCG) in adsorptive endocytosis was studied by using concanavalin A (Con A) and heterologous anti-epidermal cell serum (AES) as ligands. Isolated epidermal cells were obtained by trypsinization of guinea pig skin fragments and exposed to ferritin-conjugated Con A or AES followed by ferritin-conjugated second antibody at 37°C for various times up to 30 min. Langerhans cells among the treated epidermal cells were observed by electron microscopy. Shortly after the incubation, a few LCG in the cell periphery were filled with ferritin particles in the bulb portion. After prolonged incubation, ferritin-containing LCG increased in number and seemed to move to the juxtanuclear area. The granules were derived from plasma membrane invaginations that collected ferritin particles in a saclike extension at the end. Some of these invaginations were apparently of coated pit origin. The contents in the bulb of LCG seemed to be delivered finally to intracellular degradation compartments. Our findings clearly demonstrate that LCG are an adsorptive endocytic organelle.

Original languageEnglish
Pages (from-to)12-15
Number of pages4
JournalJournal of Investigative Dermatology
Volume85
Issue number1
Publication statusPublished - 1985
Externally publishedYes

Fingerprint

Langerhans Cells
Ferritins
Organelles
Concanavalin A
Cell membranes
Electron microscopy
Endocytosis
Serum
Skin
Electron Microscopy
Guinea Pigs
Ligands
Degradation
Cell Membrane
Antibodies

ASJC Scopus subject areas

  • Dermatology

Cite this

Takigawa, M., Iwatsuki, K., Yamada, M., Okamoto, H., & Imamura, S. (1985). The Langerhans cell granule is an adsorptive endocytic organelle. Journal of Investigative Dermatology, 85(1), 12-15.

The Langerhans cell granule is an adsorptive endocytic organelle. / Takigawa, M.; Iwatsuki, K.; Yamada, M.; Okamoto, H.; Imamura, S.

In: Journal of Investigative Dermatology, Vol. 85, No. 1, 1985, p. 12-15.

Research output: Contribution to journalArticle

Takigawa, M, Iwatsuki, K, Yamada, M, Okamoto, H & Imamura, S 1985, 'The Langerhans cell granule is an adsorptive endocytic organelle', Journal of Investigative Dermatology, vol. 85, no. 1, pp. 12-15.
Takigawa M, Iwatsuki K, Yamada M, Okamoto H, Imamura S. The Langerhans cell granule is an adsorptive endocytic organelle. Journal of Investigative Dermatology. 1985;85(1):12-15.
Takigawa, M. ; Iwatsuki, K. ; Yamada, M. ; Okamoto, H. ; Imamura, S. / The Langerhans cell granule is an adsorptive endocytic organelle. In: Journal of Investigative Dermatology. 1985 ; Vol. 85, No. 1. pp. 12-15.
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