The kinetics of induction of Hox1.6 and c-jun mRNA during three different ways of inducing differentiation in teratocarcinoma F9 cells

Souichi A. Iwai, Yukio Nishina, Mitsuko Kosaka, Tetsuro Sumi, Toshihide Doi, Masayoshi Sakuda, Yoshitake Nishimune

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Changes in Hox1.6 and c-jun gene expression were examined upon F9 cell differentiation that was induced by three independent methods: a drug treatment with retinoic acid (RA), that with sodium butyrate (NaB), and a genetic approach using the ts mutant. To obtain further information on the mechanism of teratocarcinoma cell differentiation we have examined the kinetics of the induction of Hox1.6 and c-jun mRNA whose gene products have been demonstrated to have specific roles in gene regulation. Expression of Hox1.6 mRNA was induced more rapidly than c-jun mRNA by all the above three inducing methods. Furthermore, protein synthesis was not required for the induction of Hox1.6 mRNA as well as of c-jun mRNA synthesis in all three methods. The data suggested that the transcriptional increase in the Hox1.6 mRNA was a primary response and could play an important role in F9 cell differentiation.

Original languageEnglish
Pages (from-to)462-466
Number of pages5
JournalIn Vitro Cellular & Developmental Biology - Animal: Journal of the Society for In Vitro Biology
Volume31
Issue number6
DOIs
Publication statusPublished - Jun 1995

Keywords

  • F9 cell differentiation
  • Hox1.6
  • c-jun

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology

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