The kinetics of induction of Hox1.6 and c-jun mRNA during three different ways of inducing differentiation in teratocarcinoma F9 cells

Souichi A. Iwai; Yukio Nishina; Mitsuko Kosaka; Tetsuro Sumi; Toshihide Doi; Masayoshi Sakuda; Yoshitake Nishimune

doi:10.1007/BF02634259

The kinetics of induction of Hox1.6 and c-jun mRNA during three different ways of inducing differentiation in teratocarcinoma F9 cells

Souichi A. Iwai, Yukio Nishina, Mitsuko Kosaka, Tetsuro Sumi, Toshihide Doi, Masayoshi Sakuda, Yoshitake Nishimune

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

Changes in Hox1.6 and c-jun gene expression were examined upon F9 cell differentiation that was induced by three independent methods: a drug treatment with retinoic acid (RA), that with sodium butyrate (NaB), and a genetic approach using the ts mutant. To obtain further information on the mechanism of teratocarcinoma cell differentiation we have examined the kinetics of the induction of Hox1.6 and c-jun mRNA whose gene products have been demonstrated to have specific roles in gene regulation. Expression of Hox1.6 mRNA was induced more rapidly than c-jun mRNA by all the above three inducing methods. Furthermore, protein synthesis was not required for the induction of Hox1.6 mRNA as well as of c-jun mRNA synthesis in all three methods. The data suggested that the transcriptional increase in the Hox1.6 mRNA was a primary response and could play an important role in F9 cell differentiation.

Original language	English
Pages (from-to)	462-466
Number of pages	5
Journal	In Vitro Cellular & Developmental Biology - Animal: Journal of the Society for In Vitro Biology
Volume	31
Issue number	6
DOIs	https://doi.org/10.1007/BF02634259
Publication status	Published - Jun 1995
Externally published	Yes

Keywords

F9 cell differentiation
Hox1.6
c-jun

ASJC Scopus subject areas

Developmental Biology
Cell Biology

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10.1007/BF02634259

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Iwai, S. A., Nishina, Y., Kosaka, M., Sumi, T., Doi, T., Sakuda, M., & Nishimune, Y. (1995). The kinetics of induction of Hox1.6 and c-jun mRNA during three different ways of inducing differentiation in teratocarcinoma F9 cells. In Vitro Cellular & Developmental Biology - Animal: Journal of the Society for In Vitro Biology, 31(6), 462-466. https://doi.org/10.1007/BF02634259

The kinetics of induction of Hox1.6 and c-jun mRNA during three different ways of inducing differentiation in teratocarcinoma F9 cells. / Iwai, Souichi A.; Nishina, Yukio; Kosaka, Mitsuko et al.

In: In Vitro Cellular & Developmental Biology - Animal: Journal of the Society for In Vitro Biology, Vol. 31, No. 6, 06.1995, p. 462-466.

Research output: Contribution to journal › Article › peer-review

Iwai, SA, Nishina, Y, Kosaka, M, Sumi, T, Doi, T, Sakuda, M & Nishimune, Y 1995, 'The kinetics of induction of Hox1.6 and c-jun mRNA during three different ways of inducing differentiation in teratocarcinoma F9 cells', In Vitro Cellular & Developmental Biology - Animal: Journal of the Society for In Vitro Biology, vol. 31, no. 6, pp. 462-466. https://doi.org/10.1007/BF02634259

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title = "The kinetics of induction of Hox1.6 and c-jun mRNA during three different ways of inducing differentiation in teratocarcinoma F9 cells",

abstract = "Changes in Hox1.6 and c-jun gene expression were examined upon F9 cell differentiation that was induced by three independent methods: a drug treatment with retinoic acid (RA), that with sodium butyrate (NaB), and a genetic approach using the ts mutant. To obtain further information on the mechanism of teratocarcinoma cell differentiation we have examined the kinetics of the induction of Hox1.6 and c-jun mRNA whose gene products have been demonstrated to have specific roles in gene regulation. Expression of Hox1.6 mRNA was induced more rapidly than c-jun mRNA by all the above three inducing methods. Furthermore, protein synthesis was not required for the induction of Hox1.6 mRNA as well as of c-jun mRNA synthesis in all three methods. The data suggested that the transcriptional increase in the Hox1.6 mRNA was a primary response and could play an important role in F9 cell differentiation.",

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AU - Sakuda, Masayoshi

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The kinetics of induction of Hox1.6 and c-jun mRNA during three different ways of inducing differentiation in teratocarcinoma F9 cells

Abstract

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