Abstract
The formation of ATP produced from poly(ADP-ribose) [(ADP-R)n] has been suggested to be required to repair damaged DNA. Here we investigate whether this ATP is involved in DNA replication processes during DNA repair. Poly(ADP-ribosyl)ated mid-S phase cell nuclei, which were isolated from synchronized HeLa S3 cells followed by the treatment with a DNA damaging agent, N-methyl-N′-nitro-N-nitrosoguanidine (MNNG), were revealed to retain DNA replication synthesizing activity during preincubation for de-poly(ADP-ribosyl) ation only in the presence of pyrophosphate (PPi) before DNA synthesis was started by adding 3 mM ATP. This DNA replication activity was not maintained in the presence of a potent and specific inhibitor of poly(ADP-ribose) glycohydrolase (PARG), Oenothein B (Oen B) during the preincubation with PPi. In the preincubation with PPi, μM orders of ATP was produced from (ADP-R) n. These results point to an important function of ATP generated from (ADP-R)n in nuclei for the maintenance of replication apparatus during DNA repair.
Original language | English |
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Pages (from-to) | 447-450 |
Number of pages | 4 |
Journal | Biological and Pharmaceutical Bulletin |
Volume | 30 |
Issue number | 3 |
DOIs | |
Publication status | Published - Mar 2007 |
Keywords
- (ADP-ribose)
- ATP
- DNA repair
- DNA replication synthesis
- Poly(ADP-ribose) glycohydrolase
- Poly(ADP-ribose) polymerase
ASJC Scopus subject areas
- Pharmacology
- Pharmaceutical Science