The involvement of ATP produced via (ADP-ribose)n in the maintenance of DNA replication apparatus during DNA repair

Hideharu Maruta, Naoyuki Okita, Ryoko Takasawa, Fumiaki Uchiumi, Tsutomu Hatano, Sei Ichi Tanuma

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

The formation of ATP produced from poly(ADP-ribose) [(ADP-R)n] has been suggested to be required to repair damaged DNA. Here we investigate whether this ATP is involved in DNA replication processes during DNA repair. Poly(ADP-ribosyl)ated mid-S phase cell nuclei, which were isolated from synchronized HeLa S3 cells followed by the treatment with a DNA damaging agent, N-methyl-N′-nitro-N-nitrosoguanidine (MNNG), were revealed to retain DNA replication synthesizing activity during preincubation for de-poly(ADP-ribosyl) ation only in the presence of pyrophosphate (PPi) before DNA synthesis was started by adding 3 mM ATP. This DNA replication activity was not maintained in the presence of a potent and specific inhibitor of poly(ADP-ribose) glycohydrolase (PARG), Oenothein B (Oen B) during the preincubation with PPi. In the preincubation with PPi, μM orders of ATP was produced from (ADP-R) n. These results point to an important function of ATP generated from (ADP-R)n in nuclei for the maintenance of replication apparatus during DNA repair.

Original languageEnglish
Pages (from-to)447-450
Number of pages4
JournalBiological and Pharmaceutical Bulletin
Volume30
Issue number3
DOIs
Publication statusPublished - Mar 2007

    Fingerprint

Keywords

  • (ADP-ribose)
  • ATP
  • DNA repair
  • DNA replication synthesis
  • Poly(ADP-ribose) glycohydrolase
  • Poly(ADP-ribose) polymerase

ASJC Scopus subject areas

  • Molecular Medicine
  • Pharmacology, Toxicology and Pharmaceutics(all)

Cite this