The intensely positively charged perineuronal net in the adult rat brain, with special reference to its reactions to oxine, chondroitinase ABC, hyaluronidase and collagenase

T. Murakami, M. Kosaka, H. Sato, Aiji Ohtsuka, Takehito Taguchi

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Light microscopic observations of healthy adult rat brain sections stained with anionic iron colloid indicated that 5-10% of neurons in the hippocampal subiculum and all neurons in the medial cerebellar nucleus possessed an intensely positively charged perineuronal net. This net was demonstrated to react to oxine, and therefore suggested to consist of guanidino compounds. It was further shown that the intensely positively charged perineuronal net, in accordance with the intensely negatively charged perineuronal net of proteoglycans, was digested by chondroitinase ABC, hyaluronidase, and collagenase, but not by endo-alpha-N-acetylgalactosaminidase. This finding suggested that the former positively charged net might be linked to the latter negatively charged one.

Original languageEnglish
Pages (from-to)313-318
Number of pages6
JournalArchives of Histology and Cytology
Volume64
Issue number3
Publication statusPublished - 2001

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Chondroitin ABC Lyase
Oxyquinoline
Hyaluronoglucosaminidase
Collagenases
alpha-N-Acetylgalactosaminidase
Neurons
Cerebellar Nuclei
Brain
Colloids
Proteoglycans
Hippocampus
Iron
Light

ASJC Scopus subject areas

  • Anatomy
  • Histology

Cite this

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title = "The intensely positively charged perineuronal net in the adult rat brain, with special reference to its reactions to oxine, chondroitinase ABC, hyaluronidase and collagenase",
abstract = "Light microscopic observations of healthy adult rat brain sections stained with anionic iron colloid indicated that 5-10{\%} of neurons in the hippocampal subiculum and all neurons in the medial cerebellar nucleus possessed an intensely positively charged perineuronal net. This net was demonstrated to react to oxine, and therefore suggested to consist of guanidino compounds. It was further shown that the intensely positively charged perineuronal net, in accordance with the intensely negatively charged perineuronal net of proteoglycans, was digested by chondroitinase ABC, hyaluronidase, and collagenase, but not by endo-alpha-N-acetylgalactosaminidase. This finding suggested that the former positively charged net might be linked to the latter negatively charged one.",
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T1 - The intensely positively charged perineuronal net in the adult rat brain, with special reference to its reactions to oxine, chondroitinase ABC, hyaluronidase and collagenase

AU - Murakami, T.

AU - Kosaka, M.

AU - Sato, H.

AU - Ohtsuka, Aiji

AU - Taguchi, Takehito

PY - 2001

Y1 - 2001

N2 - Light microscopic observations of healthy adult rat brain sections stained with anionic iron colloid indicated that 5-10% of neurons in the hippocampal subiculum and all neurons in the medial cerebellar nucleus possessed an intensely positively charged perineuronal net. This net was demonstrated to react to oxine, and therefore suggested to consist of guanidino compounds. It was further shown that the intensely positively charged perineuronal net, in accordance with the intensely negatively charged perineuronal net of proteoglycans, was digested by chondroitinase ABC, hyaluronidase, and collagenase, but not by endo-alpha-N-acetylgalactosaminidase. This finding suggested that the former positively charged net might be linked to the latter negatively charged one.

AB - Light microscopic observations of healthy adult rat brain sections stained with anionic iron colloid indicated that 5-10% of neurons in the hippocampal subiculum and all neurons in the medial cerebellar nucleus possessed an intensely positively charged perineuronal net. This net was demonstrated to react to oxine, and therefore suggested to consist of guanidino compounds. It was further shown that the intensely positively charged perineuronal net, in accordance with the intensely negatively charged perineuronal net of proteoglycans, was digested by chondroitinase ABC, hyaluronidase, and collagenase, but not by endo-alpha-N-acetylgalactosaminidase. This finding suggested that the former positively charged net might be linked to the latter negatively charged one.

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