Abstract
We have previously reported an important excitatory role of the perirhinal cortex (PRC) in rat kindling development using an immunohistochemistry technique. In this study, we investigated the roles of the PRC and the insular cortex (INS) located rostral to the PRC, in fully-kindled amygdaloid seizures, using a microinjection technique in the rat kindling model of epilepsy. Following the establishment of daily kindling, we investigated the effects of microinjections of procaine hydrochloride, 2-amino-5-phosphonovalerate (APV; an N-methyl-D-aspartate (NMDA) receptor antagonist) and 2,3-dihydroxy-6-nitro-7-sulfamoyl-benzo(f)-quinoxaline (NBQX; an α-amino-3-hydroxy-5-methyl-isoxazole-4-propionic acid (AMPA) receptor antagonist). Microinjections of these drugs into the ipsilateral PRC did not suppress kindled seizures. The possibility is that the process of kindling development forms novel seizure-generalization pathways that do not require further activation of the PRC. On the other hand, procaine and APV injected into the ipsilateral INS significantly suppressed kindled seizures. The manner of suppression appeared to be 'all or none'. It is therefore possible that at least the activation of NMDA receptors in the INS is necessary to express generalized kindled amygdaloid seizures.
| Original language | English |
|---|---|
| Pages (from-to) | 169-178 |
| Number of pages | 10 |
| Journal | Epilepsy Research |
| Volume | 46 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 2001 |
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Keywords
- Insular cortex
- Kindling
- Microinjection
- Perirhinal cortex
- Seizure generalization
ASJC Scopus subject areas
- Clinical Neurology
- Pediatrics, Perinatology, and Child Health
- Neurology
Cite this
The insular but not the perirhinal cortex is involved in the expression of fully-kindled amygdaloid seizures in rats. / Kodama, Masazumi; Yamada, Norihito; Sato, Keiko; Sato, Toshiki; Morimoto, Kiyoshi; Kuroda, Shigetoshi.
In: Epilepsy Research, Vol. 46, No. 2, 2001, p. 169-178.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The insular but not the perirhinal cortex is involved in the expression of fully-kindled amygdaloid seizures in rats
AU - Kodama, Masazumi
AU - Yamada, Norihito
AU - Sato, Keiko
AU - Sato, Toshiki
AU - Morimoto, Kiyoshi
AU - Kuroda, Shigetoshi
PY - 2001
Y1 - 2001
N2 - We have previously reported an important excitatory role of the perirhinal cortex (PRC) in rat kindling development using an immunohistochemistry technique. In this study, we investigated the roles of the PRC and the insular cortex (INS) located rostral to the PRC, in fully-kindled amygdaloid seizures, using a microinjection technique in the rat kindling model of epilepsy. Following the establishment of daily kindling, we investigated the effects of microinjections of procaine hydrochloride, 2-amino-5-phosphonovalerate (APV; an N-methyl-D-aspartate (NMDA) receptor antagonist) and 2,3-dihydroxy-6-nitro-7-sulfamoyl-benzo(f)-quinoxaline (NBQX; an α-amino-3-hydroxy-5-methyl-isoxazole-4-propionic acid (AMPA) receptor antagonist). Microinjections of these drugs into the ipsilateral PRC did not suppress kindled seizures. The possibility is that the process of kindling development forms novel seizure-generalization pathways that do not require further activation of the PRC. On the other hand, procaine and APV injected into the ipsilateral INS significantly suppressed kindled seizures. The manner of suppression appeared to be 'all or none'. It is therefore possible that at least the activation of NMDA receptors in the INS is necessary to express generalized kindled amygdaloid seizures.
AB - We have previously reported an important excitatory role of the perirhinal cortex (PRC) in rat kindling development using an immunohistochemistry technique. In this study, we investigated the roles of the PRC and the insular cortex (INS) located rostral to the PRC, in fully-kindled amygdaloid seizures, using a microinjection technique in the rat kindling model of epilepsy. Following the establishment of daily kindling, we investigated the effects of microinjections of procaine hydrochloride, 2-amino-5-phosphonovalerate (APV; an N-methyl-D-aspartate (NMDA) receptor antagonist) and 2,3-dihydroxy-6-nitro-7-sulfamoyl-benzo(f)-quinoxaline (NBQX; an α-amino-3-hydroxy-5-methyl-isoxazole-4-propionic acid (AMPA) receptor antagonist). Microinjections of these drugs into the ipsilateral PRC did not suppress kindled seizures. The possibility is that the process of kindling development forms novel seizure-generalization pathways that do not require further activation of the PRC. On the other hand, procaine and APV injected into the ipsilateral INS significantly suppressed kindled seizures. The manner of suppression appeared to be 'all or none'. It is therefore possible that at least the activation of NMDA receptors in the INS is necessary to express generalized kindled amygdaloid seizures.
KW - Insular cortex
KW - Kindling
KW - Microinjection
KW - Perirhinal cortex
KW - Seizure generalization
UR - http://www.scopus.com/inward/record.url?scp=0034906742&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0034906742&partnerID=8YFLogxK
U2 - 10.1016/S0920-1211(01)00279-0
DO - 10.1016/S0920-1211(01)00279-0
M3 - Article
C2 - 11463518
AN - SCOPUS:0034906742
VL - 46
SP - 169
EP - 178
JO - Epilepsy Research
JF - Epilepsy Research
SN - 0920-1211
IS - 2
ER -