The inclusion of blastomeres into the inner cell mass in early-stage human embryos depends on the sequence of cell cleavages during the fourth division

Junko Otsuki; Toshiroh Iwasaki; Noritoshi Enatsu; Yuya Katada; Kohyu Furuhashi; Masahide Shiotani

doi:10.1371/journal.pone.0240936

The inclusion of blastomeres into the inner cell mass in early-stage human embryos depends on the sequence of cell cleavages during the fourth division

Junko Otsuki, Toshiroh Iwasaki, Noritoshi Enatsu, Yuya Katada, Kohyu Furuhashi, Masahide Shiotani

Faculty of Agriculture

Research output: Contribution to journal › Article › peer-review

Abstract

The fate of the ICM in humans is still unknown, due to the ethical difficulties surrounding experimentation in this field. In this study we have explored the existing time-lapse recording data of embryos in the early stages of development, taking advantage of the large refractile bodies (RBs) within blastomeres as cellular markers. Our study found that the cellular composition of the ICM in humans is largely determined at the time of the fourth division and blastomeres which cleave first to fourth, during the fourth division from 8 cells to 16 cells, have the potential to be incorporated in the ICM.

Original language	English
Article number	e0240936
Journal	PloS one
Volume	15
Issue number	10 October
DOIs	https://doi.org/10.1371/journal.pone.0240936
Publication status	Published - Oct 2020

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology(all)
Agricultural and Biological Sciences(all)
General

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10.1371/journal.pone.0240936

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abstract = "The fate of the ICM in humans is still unknown, due to the ethical difficulties surrounding experimentation in this field. In this study we have explored the existing time-lapse recording data of embryos in the early stages of development, taking advantage of the large refractile bodies (RBs) within blastomeres as cellular markers. Our study found that the cellular composition of the ICM in humans is largely determined at the time of the fourth division and blastomeres which cleave first to fourth, during the fourth division from 8 cells to 16 cells, have the potential to be incorporated in the ICM.",

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AU - Enatsu, Noritoshi

AU - Katada, Yuya

AU - Furuhashi, Kohyu

AU - Shiotani, Masahide

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The inclusion of blastomeres into the inner cell mass in early-stage human embryos depends on the sequence of cell cleavages during the fourth division

Abstract

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