The in vivo Inhibition of Oral Biofilm Accumulation and Streptococcus mutans by Ceramic Water

Ryota Nomura, Risa Yoneyama, Shuhei Naka, Masatoshi Otsugu, Yuko Ogaya, Rina Hatakeyama, Yumiko Morita, Jumpei Maruo, Michiyo Nakano, Osamu Yamada, Kazuhiko Nakano

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Combustion-synthesized titanium carbide ceramics uniformly disperse silver, producing silver ions and hydroxyl radicals in water. This generates antimicrobial activity against various bacteria. One such bacterium is Streptococcus mutans, a gram-positive anaerobic bacterium known as a major pathogen of dental caries. In this study, we analyzed the inhibition of oral biofilms and S. mutans by ceramic water in in vitro and human studies. S. mutans strains showed significantly lower antimicrobial and sucrose-dependent adhesion activity in the presence of ceramic powder compared with untreated culture medium. Confocal microscopy revealed that S. mutans biofilm structures with ceramic powder were thin and coarse. Twenty-seven volunteers (13 males, 14 females; 18-37 years old, mean 25.2 years) were enrolled for subsequent studies. After each meal, one group was asked to rinse with ceramic water while the other rinsed with untreated water for 1 week. After 1 week, the rinsing contents were switched between the groups and the same protocol was followed for an additional week. After rinsing with ceramic water, the average plaque score was 43.0 ± 3.7, which was significantly lower than the baseline value (74.1 ± 5.7, p < 0.001). However, no significant difference was observed when rinsing with untreated water. In addition, the total number of S. mutans in saliva was significantly reduced after rinsing with ceramic water compared with untreated water (p < 0.05). These results suggest that ceramic water possesses antimicrobial activity against S. mutans and inhibits biofilm formation. Rinsing with ceramic water can also inhibit dental plaque formation and S. mutans colonization in humans.

Original languageEnglish
Pages (from-to)58-67
Number of pages10
JournalCaries Research
Volume51
Issue number1
DOIs
Publication statusPublished - Mar 1 2017

Fingerprint

Streptococcus mutans
Ceramics
Biofilms
Water
Silver
Powders
Bacteria
Dental Plaque
Anaerobic Bacteria
Dental Caries
Gram-Positive Bacteria
Saliva
Confocal Microscopy
Hydroxyl Radical
Culture Media
Meals
Sucrose
Volunteers
Ions

Keywords

  • Biofilms
  • Ceramic water
  • Dental caries
  • Dental plaque
  • Streptococcus mutans

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Nomura, R., Yoneyama, R., Naka, S., Otsugu, M., Ogaya, Y., Hatakeyama, R., ... Nakano, K. (2017). The in vivo Inhibition of Oral Biofilm Accumulation and Streptococcus mutans by Ceramic Water. Caries Research, 51(1), 58-67. https://doi.org/10.1159/000452343

The in vivo Inhibition of Oral Biofilm Accumulation and Streptococcus mutans by Ceramic Water. / Nomura, Ryota; Yoneyama, Risa; Naka, Shuhei; Otsugu, Masatoshi; Ogaya, Yuko; Hatakeyama, Rina; Morita, Yumiko; Maruo, Jumpei; Nakano, Michiyo; Yamada, Osamu; Nakano, Kazuhiko.

In: Caries Research, Vol. 51, No. 1, 01.03.2017, p. 58-67.

Research output: Contribution to journalArticle

Nomura, R, Yoneyama, R, Naka, S, Otsugu, M, Ogaya, Y, Hatakeyama, R, Morita, Y, Maruo, J, Nakano, M, Yamada, O & Nakano, K 2017, 'The in vivo Inhibition of Oral Biofilm Accumulation and Streptococcus mutans by Ceramic Water', Caries Research, vol. 51, no. 1, pp. 58-67. https://doi.org/10.1159/000452343
Nomura, Ryota ; Yoneyama, Risa ; Naka, Shuhei ; Otsugu, Masatoshi ; Ogaya, Yuko ; Hatakeyama, Rina ; Morita, Yumiko ; Maruo, Jumpei ; Nakano, Michiyo ; Yamada, Osamu ; Nakano, Kazuhiko. / The in vivo Inhibition of Oral Biofilm Accumulation and Streptococcus mutans by Ceramic Water. In: Caries Research. 2017 ; Vol. 51, No. 1. pp. 58-67.
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