The in vitro life-span of human periodontal ligament fibroblasts

Yoshihiko Sawa, A. Phillips, J. Hollard, S. Yoshida, M. W. Braithwaite

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The in vitro life-span of human periodontal ligament fibroblasts (PDLF) was studied on clones from periodontium of teeth extracted due to periodontitis and dental caries (69 clones/192 individuals, aged 20-80 years) and from periodontium of teeth extracted for orthodontic reasons (23 clones/26 individuals, aged 15-19 years). In the primary cultures the ratio of the number of cells expressing senescence-associated β-galactosidase (SA-β-Gal) to the total number of cells is significantly larger in PDLF (92 clones; 11.1±4.9%) than in human gingival fibroblasts (GF) (10 clones; 0.5±0.1%). The finite population doubling numbers (PD) of PDLF are not age-matched and the mean PD of PDLF (7.1±2.9) is significantly smaller than GF (28.5±3.2), IMR-90 (human lung fibroblasts, 5 clones; 44.3±2.2), and human osteoblasts (5 clones; 19.7±1.4). Comparing the ratio of the number of SA-β-Gal positive cells to the total number of cells in primary culture, and the finite PD in PDLF cultures: 1) the ratio of 15-19 years old donor group is significantly smaller than in the other donor groups (20-29, 30-39, 40-49, 50-59 and 60-80 years old) and 2) there were no statistically significant differences among the 20-29, 30-39, 40-49 and 50-59 year old donor groups, and the 30-39, 40-49, 50-59 and 60-80 year old donor groups. These findings suggest that the in vitro life-span of PDLF is shorter than other fibroblasts in the connective tissues and that PDLF may undergo senescence in adult clones without relation to donor's age. There may be more aged fibroblasts in periodontium than in other tissues, such as gingiva and lung. (C) 2000 Harcourt Publishers Ltd.

Original languageEnglish
Pages (from-to)163-170
Number of pages8
JournalTissue and Cell
Volume32
Issue number2
DOIs
Publication statusPublished - 2000
Externally publishedYes

Fingerprint

Periodontal Ligament
Fibroblasts
Clone Cells
Periodontium
Galactosidases
In Vitro Techniques
Tooth
Population
Lung
Primary Cell Culture
Cell Aging
Periodontitis
Dental Caries
Gingiva
Orthodontics
Osteoblasts
Connective Tissue
Cell Count

Keywords

  • Life-span
  • Periodontal ligament fibroblasts
  • Senescent-associated β-galactosidase

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology

Cite this

Sawa, Y., Phillips, A., Hollard, J., Yoshida, S., & Braithwaite, M. W. (2000). The in vitro life-span of human periodontal ligament fibroblasts. Tissue and Cell, 32(2), 163-170. https://doi.org/10.1054/tice.2000.0100

The in vitro life-span of human periodontal ligament fibroblasts. / Sawa, Yoshihiko; Phillips, A.; Hollard, J.; Yoshida, S.; Braithwaite, M. W.

In: Tissue and Cell, Vol. 32, No. 2, 2000, p. 163-170.

Research output: Contribution to journalArticle

Sawa, Y, Phillips, A, Hollard, J, Yoshida, S & Braithwaite, MW 2000, 'The in vitro life-span of human periodontal ligament fibroblasts', Tissue and Cell, vol. 32, no. 2, pp. 163-170. https://doi.org/10.1054/tice.2000.0100
Sawa, Yoshihiko ; Phillips, A. ; Hollard, J. ; Yoshida, S. ; Braithwaite, M. W. / The in vitro life-span of human periodontal ligament fibroblasts. In: Tissue and Cell. 2000 ; Vol. 32, No. 2. pp. 163-170.
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