Abstract
Interleukin (IL)-18, which is elevated in the plasma during acute rejection after organ transplantation, is known to induce the expression of intercellular adhesion molecule (ICAM)-1, B7.1, B7.2, CD40 and CD40 ligand (CD40L) on monocytes, the production of interferon (IFN)-γ and IL-12 and the proliferation of lymphocytes during the human mixed lymphocyte reaction (MLR). Ciprofloxacin (CIP), which is useful for the clinical treatment of infections due to its antibacterial properties after transplantation, was shown to suppress the IFN-γ and IL-12 production, the lymphocyte proliferation and the ICAM-1, B7.1, B7.2 and CD40 expression on monocytes during MLR in the presence of IL-18. CIP also induced the production of prostaglandin (PG) E2. In order to determine whether the effects of CIP on the expression of the activation markers were due to CIP-dependent production of PGE2, we examined the effect of cyclooxygenase (COX)-2 and protein kinase A (PKA) inhibitors on the actions of CIP. Thereby, the inhibitors were found to abolish the actions of CIP. These results therefore suggest that CIP might exert its immune modulatory effects via the production of PGE2.
| Original language | English |
|---|---|
| Pages (from-to) | 110-119 |
| Number of pages | 10 |
| Journal | Clinical Immunology |
| Volume | 119 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Apr 2006 |
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Keywords
- Ciprofloxacin
- Costimulatory molecule
- IL-18
- MLR
- Prostaglandin
ASJC Scopus subject areas
- Immunology
- Immunology and Allergy
Cite this
The immunosuppressive effects of ciprofloxacin during human mixed lymphocyte reaction. / Katsuno, Goutarou; Takahashi, Hideo Kohka; Iwagaki, Hiromi; Mizuno, Kenji; Yagi, Takahito; Mori, Shuji; Saito, Shinya; Yoshino, Tadashi; Nishibori, Masahiro; Tanaka, Noriaki.
In: Clinical Immunology, Vol. 119, No. 1, 04.2006, p. 110-119.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The immunosuppressive effects of ciprofloxacin during human mixed lymphocyte reaction
AU - Katsuno, Goutarou
AU - Takahashi, Hideo Kohka
AU - Iwagaki, Hiromi
AU - Mizuno, Kenji
AU - Yagi, Takahito
AU - Mori, Shuji
AU - Saito, Shinya
AU - Yoshino, Tadashi
AU - Nishibori, Masahiro
AU - Tanaka, Noriaki
PY - 2006/4
Y1 - 2006/4
N2 - Interleukin (IL)-18, which is elevated in the plasma during acute rejection after organ transplantation, is known to induce the expression of intercellular adhesion molecule (ICAM)-1, B7.1, B7.2, CD40 and CD40 ligand (CD40L) on monocytes, the production of interferon (IFN)-γ and IL-12 and the proliferation of lymphocytes during the human mixed lymphocyte reaction (MLR). Ciprofloxacin (CIP), which is useful for the clinical treatment of infections due to its antibacterial properties after transplantation, was shown to suppress the IFN-γ and IL-12 production, the lymphocyte proliferation and the ICAM-1, B7.1, B7.2 and CD40 expression on monocytes during MLR in the presence of IL-18. CIP also induced the production of prostaglandin (PG) E2. In order to determine whether the effects of CIP on the expression of the activation markers were due to CIP-dependent production of PGE2, we examined the effect of cyclooxygenase (COX)-2 and protein kinase A (PKA) inhibitors on the actions of CIP. Thereby, the inhibitors were found to abolish the actions of CIP. These results therefore suggest that CIP might exert its immune modulatory effects via the production of PGE2.
AB - Interleukin (IL)-18, which is elevated in the plasma during acute rejection after organ transplantation, is known to induce the expression of intercellular adhesion molecule (ICAM)-1, B7.1, B7.2, CD40 and CD40 ligand (CD40L) on monocytes, the production of interferon (IFN)-γ and IL-12 and the proliferation of lymphocytes during the human mixed lymphocyte reaction (MLR). Ciprofloxacin (CIP), which is useful for the clinical treatment of infections due to its antibacterial properties after transplantation, was shown to suppress the IFN-γ and IL-12 production, the lymphocyte proliferation and the ICAM-1, B7.1, B7.2 and CD40 expression on monocytes during MLR in the presence of IL-18. CIP also induced the production of prostaglandin (PG) E2. In order to determine whether the effects of CIP on the expression of the activation markers were due to CIP-dependent production of PGE2, we examined the effect of cyclooxygenase (COX)-2 and protein kinase A (PKA) inhibitors on the actions of CIP. Thereby, the inhibitors were found to abolish the actions of CIP. These results therefore suggest that CIP might exert its immune modulatory effects via the production of PGE2.
KW - Ciprofloxacin
KW - Costimulatory molecule
KW - IL-18
KW - MLR
KW - Prostaglandin
UR - http://www.scopus.com/inward/record.url?scp=33644770042&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33644770042&partnerID=8YFLogxK
U2 - 10.1016/j.clim.2005.12.004
DO - 10.1016/j.clim.2005.12.004
M3 - Article
C2 - 16458073
AN - SCOPUS:33644770042
VL - 119
SP - 110
EP - 119
JO - Clinical Immunology
JF - Clinical Immunology
SN - 1521-6616
IS - 1
ER -