TY - JOUR
T1 - The immunoreceptor SLAMF8 promotes the differentiation of follicular dendritic cell-dependent monocytic cells with B cell-activating ability
AU - Magari, Masaki
AU - Nishioka, Miku
AU - Hari, Tomomi
AU - Ogawa, Sayaka
AU - Takahashi, Kaho
AU - Hatano, Naoya
AU - Kanayama, Naoki
AU - Futami, Junichiro
AU - Tokumitsu, Hiroshi
N1 - Funding Information:
The present study was supported by a Grant‐in‐Aid for Scientific Research (18K04852 and 21K04791) from the Japan Society for the Promotion of Science, Takeda Science Foundation, and the Ryobi Teien Memory Foundation to MM.
Publisher Copyright:
© 2022 Federation of European Biochemical Societies.
PY - 2022
Y1 - 2022
N2 - Follicular dendritic cells (FDCs) play a crucial role in generating high-affinity antibody-producing B cells during the germinal center (GC) reaction. Herein, we analysed the altered gene expression profile of a mouse FDC line, FL-Y, following lymphotoxin β receptor stimulation, and observed increased Slam-family member 8 (Slamf8) mRNA expression. Forced Slamf8 expression and SLAMF8-Fc addition enhanced the ability of FL-Y cells to induce FDC-induced monocytic cell (FDMC) differentiation. FDMCs accelerated GC-phenotype proliferation in cultured B cells, suggesting that they are capable of promoting GC responses. Furthermore, a pulldown assay showed that SLAMF8-Fc could bind to SLAMF8-His. Overall, the homophilic interaction of SLAMF8 promotes FDMC differentiation and SLAMF8 might act as a novel regulator of GC responses by regulating FDMC differentiation.
AB - Follicular dendritic cells (FDCs) play a crucial role in generating high-affinity antibody-producing B cells during the germinal center (GC) reaction. Herein, we analysed the altered gene expression profile of a mouse FDC line, FL-Y, following lymphotoxin β receptor stimulation, and observed increased Slam-family member 8 (Slamf8) mRNA expression. Forced Slamf8 expression and SLAMF8-Fc addition enhanced the ability of FL-Y cells to induce FDC-induced monocytic cell (FDMC) differentiation. FDMCs accelerated GC-phenotype proliferation in cultured B cells, suggesting that they are capable of promoting GC responses. Furthermore, a pulldown assay showed that SLAMF8-Fc could bind to SLAMF8-His. Overall, the homophilic interaction of SLAMF8 promotes FDMC differentiation and SLAMF8 might act as a novel regulator of GC responses by regulating FDMC differentiation.
KW - follicular dendritic cell
KW - follicular dendritic cell-induced monocytic cell
KW - germinal center
KW - lymphotoxin
KW - lymphotoxin β receptor
KW - SLAMF8
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U2 - 10.1002/1873-3468.14468
DO - 10.1002/1873-3468.14468
M3 - Article
C2 - 35953458
AN - SCOPUS:85136510230
SN - 0014-5793
JO - FEBS Letters
JF - FEBS Letters
ER -