The hydA gene encoding the H2-evolving hydrogenase of Clostridium perfringens: Molecular characterization and expression of the gene

Masato Kaji, Yuki Taniguchi, Osamu Matsushita, Seiichi Katayama, Shigeru Miyata, Shushi Morita, Akinobu Okabe

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

A putative hydrogenase (hydA) gene of Clostridium perfringens encodes a protein with strong identity to Clostridium pasteurianum hydrogenase I. Disruption of the hydA gene abolished H2 productivity, confirming its function. A putative butyrate kinase gene (buk) is adjacent to the hydA gene. When cultures were grown in medium with glucose, 1.8-kb hydA and 2.1-kb buk transcripts and a 3.9-kb transcript hybridized with both hydA and buk-probe were detectable in all the exponential growth phases. In medium without glucose, these transcripts were decreased rapidly after the mid-exponential phase. These results suggest that the transcription of these two genes is probably regulated by a similar mechanism in response to glucose availability. Copyright (C) 1999 Federation of European Microbiological Societies.

Original languageEnglish
Pages (from-to)329-336
Number of pages8
JournalFEMS Microbiology Letters
Volume181
Issue number2
DOIs
Publication statusPublished - Dec 15 1999
Externally publishedYes

Keywords

  • Clostridium perfringens
  • Fermentation
  • Gene disruption
  • Hydrogen gas
  • Hydrogenase

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology
  • Genetics

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