The hydA gene encoding the H2-evolving hydrogenase of Clostridium perfringens: Molecular characterization and expression of the gene

Masato Kaji, Yuki Taniguchi, Osamu Matsushita, Seiichi Katayama, Shigeru Miyata, Shushi Morita, Akinobu Okabe

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

A putative hydrogenase (hydA) gene of Clostridium perfringens encodes a protein with strong identity to Clostridium pasteurianum hydrogenase I. Disruption of the hydA gene abolished H2 productivity, confirming its function. A putative butyrate kinase gene (buk) is adjacent to the hydA gene. When cultures were grown in medium with glucose, 1.8-kb hydA and 2.1-kb buk transcripts and a 3.9-kb transcript hybridized with both hydA and buk-probe were detectable in all the exponential growth phases. In medium without glucose, these transcripts were decreased rapidly after the mid-exponential phase. These results suggest that the transcription of these two genes is probably regulated by a similar mechanism in response to glucose availability. Copyright (C) 1999 Federation of European Microbiological Societies.

Original languageEnglish
Pages (from-to)329-336
Number of pages8
JournalFEMS Microbiology Letters
Volume181
Issue number2
DOIs
Publication statusPublished - Dec 15 1999
Externally publishedYes

Fingerprint

Hydrogenase
Clostridium perfringens
Gene Expression
Genes
Glucose
Clostridium
Growth

Keywords

  • Clostridium perfringens
  • Fermentation
  • Gene disruption
  • Hydrogen gas
  • Hydrogenase

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Applied Microbiology and Biotechnology
  • Microbiology

Cite this

The hydA gene encoding the H2-evolving hydrogenase of Clostridium perfringens : Molecular characterization and expression of the gene. / Kaji, Masato; Taniguchi, Yuki; Matsushita, Osamu; Katayama, Seiichi; Miyata, Shigeru; Morita, Shushi; Okabe, Akinobu.

In: FEMS Microbiology Letters, Vol. 181, No. 2, 15.12.1999, p. 329-336.

Research output: Contribution to journalArticle

Kaji, Masato ; Taniguchi, Yuki ; Matsushita, Osamu ; Katayama, Seiichi ; Miyata, Shigeru ; Morita, Shushi ; Okabe, Akinobu. / The hydA gene encoding the H2-evolving hydrogenase of Clostridium perfringens : Molecular characterization and expression of the gene. In: FEMS Microbiology Letters. 1999 ; Vol. 181, No. 2. pp. 329-336.
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