The HvNramp5 transporter mediates uptake of cadmium and manganese, but not iron

Dezhi Wu, Naoki Yamaji, Miki Yamane, Miho Kashino-Fujii, Kazuhiro Sato, Jian Feng Ma

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

The Natural Resistance Associated Macrophage Protein (Nramp) represents a transporter family for metal ions in all organisms. Here, we functionally characterized a member of Nramp family in barley (Hordeum vulgare), HvNramp5. This member showed different expression patterns, transport substrate specificity, and cellular localization from its close homolog in rice (Oryza sativa), OsNramp5, although HvNramp5 was also localized to the plasma membrane. HvNramp5 was mainly expressed in the roots and its expression was not affected by Cd and deficiency of Zn, Cu, and Mn, but slightly up-regulated by Fe deficiency. Spatial expression analysis showed that the expression of HvNramp5 was higher in the root tips than that in the basal root regions. Furthermore, analysis with laser microdissection revealed higher expression of HvNramp5 in the outer root cell layers. HvNramp5 showed transport activity for both Mn2+ and Cd2+, but not for Fe2+when expressed in yeast. Immunostaining with a HvNramp5 antibody showed that this protein was localized in the root epidermal cells without polarity. Knockdown of HvNramp5 in barley resulted in a significant reduction in the seedling growth at low Mn supply, but this reduction was rescued at high Mn supply. The concentration of Mn and Cd, but not other metals including Cu, Zn, and Fe, was decreased in both the roots and shoots of knockdown lines compared with the wild-type barley. These results indicate that HvNramp5 is a transporter required for uptake of Mn and Cd, but not for Fe, and that barley has a distinct uptake system from rice.

Original languageEnglish
Pages (from-to)1899-1910
Number of pages12
JournalPlant Physiology
Volume172
Issue number3
DOIs
Publication statusPublished - Nov 1 2016

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Hordeum
Manganese
Cadmium
manganese
transporters
cadmium
Iron
iron
uptake mechanisms
barley
macrophages
Metals
rice
Microdissection
Cell Polarity
Spatial Analysis
Meristem
proteins
substrate specificity
Substrate Specificity

ASJC Scopus subject areas

  • Physiology
  • Genetics
  • Plant Science

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The HvNramp5 transporter mediates uptake of cadmium and manganese, but not iron. / Wu, Dezhi; Yamaji, Naoki; Yamane, Miki; Kashino-Fujii, Miho; Sato, Kazuhiro; Ma, Jian Feng.

In: Plant Physiology, Vol. 172, No. 3, 01.11.2016, p. 1899-1910.

Research output: Contribution to journalArticle

Wu, Dezhi ; Yamaji, Naoki ; Yamane, Miki ; Kashino-Fujii, Miho ; Sato, Kazuhiro ; Ma, Jian Feng. / The HvNramp5 transporter mediates uptake of cadmium and manganese, but not iron. In: Plant Physiology. 2016 ; Vol. 172, No. 3. pp. 1899-1910.
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