The human chondrosarcoma HCS-2/8 cell line is responsive to BMP-7, but not to IL-1beta

Joachim Saas, Matthias Gebauer, Carsten Jacobi, Jochen Haag, Masaharu Takigawa, Thomas Aigner

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Cultures of primary chondrocytes as in vitro model systems for studying the cellular behavior of chondrocytes are notoriously difficult to cultivate and propagate. One way to circumvent these problems appears to be the use of immortalized/immortal chondrocytic cell lines. In the present study, we were interested whether the chondrosarcoma derived HCS-2/8 cells are suitable for studying major cellular reaction pattern in response to key anabolic (BMP-7) and catabolic (IL-1beta) factors. Therefore, we used cDNA array and real-time PCR technology in order to evaluate gene expression triggerd by stimulation with IL-1beta (0,1-100 ng/ml) and BMP-7 in confluent monolayer cultures. HCS-2/8 cells hardly responded to IL-1beta, but showed good responsiveness to BMP-7. We found 12 genes up- and 17 significantly down-regulated by BMP-7 (out of 340 investigated genes). Besides the expected activation of anabolic genes chondrocytic cells after BMP-stimulation try to neutralize activation of the BMP-signalling cascade by expressing intra- and extracellular BMP-antagonists. Chondrosarcoma derived cell lines are a potential substitute for primary articular chondrocytes promising consistent expression of a differentiated chondrocyte phenotype with sufficient proliferative capacity. However, as shown by this study one needs to carefully select the cell line depending on the effects which one intends to study. In this respect, HCS-2/8 cells are a validated tool for studying BMP-effects on chondrocytes, but not e.g. effects of interleukin-1.

Original languageEnglish
Pages (from-to)2027-2035
Number of pages9
JournalFrontiers in Bioscience
Volume10
Issue numberSUPPL. 1
Publication statusPublished - 2005

Fingerprint

Bone Morphogenetic Protein 7
Chondrosarcoma
Chondrocytes
Cells
Cell Line
Genes
Chemical activation
Interleukin-1
Gene expression
Monolayers
Oligonucleotide Array Sequence Analysis
Complementary DNA
Transcriptional Activation
Real-Time Polymerase Chain Reaction
Joints
Technology
Phenotype
Gene Expression

Keywords

  • BMP
  • Cartilage
  • Cell Line
  • Chondrocyte
  • Chondrosarcoma
  • DNA array
  • Growth Factors
  • IL-1
  • Microarray

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Saas, J., Gebauer, M., Jacobi, C., Haag, J., Takigawa, M., & Aigner, T. (2005). The human chondrosarcoma HCS-2/8 cell line is responsive to BMP-7, but not to IL-1beta. Frontiers in Bioscience, 10(SUPPL. 1), 2027-2035.

The human chondrosarcoma HCS-2/8 cell line is responsive to BMP-7, but not to IL-1beta. / Saas, Joachim; Gebauer, Matthias; Jacobi, Carsten; Haag, Jochen; Takigawa, Masaharu; Aigner, Thomas.

In: Frontiers in Bioscience, Vol. 10, No. SUPPL. 1, 2005, p. 2027-2035.

Research output: Contribution to journalArticle

Saas, J, Gebauer, M, Jacobi, C, Haag, J, Takigawa, M & Aigner, T 2005, 'The human chondrosarcoma HCS-2/8 cell line is responsive to BMP-7, but not to IL-1beta', Frontiers in Bioscience, vol. 10, no. SUPPL. 1, pp. 2027-2035.
Saas, Joachim ; Gebauer, Matthias ; Jacobi, Carsten ; Haag, Jochen ; Takigawa, Masaharu ; Aigner, Thomas. / The human chondrosarcoma HCS-2/8 cell line is responsive to BMP-7, but not to IL-1beta. In: Frontiers in Bioscience. 2005 ; Vol. 10, No. SUPPL. 1. pp. 2027-2035.
@article{1e6891a4c4f74d3093555f6faa768025,
title = "The human chondrosarcoma HCS-2/8 cell line is responsive to BMP-7, but not to IL-1beta",
abstract = "Cultures of primary chondrocytes as in vitro model systems for studying the cellular behavior of chondrocytes are notoriously difficult to cultivate and propagate. One way to circumvent these problems appears to be the use of immortalized/immortal chondrocytic cell lines. In the present study, we were interested whether the chondrosarcoma derived HCS-2/8 cells are suitable for studying major cellular reaction pattern in response to key anabolic (BMP-7) and catabolic (IL-1beta) factors. Therefore, we used cDNA array and real-time PCR technology in order to evaluate gene expression triggerd by stimulation with IL-1beta (0,1-100 ng/ml) and BMP-7 in confluent monolayer cultures. HCS-2/8 cells hardly responded to IL-1beta, but showed good responsiveness to BMP-7. We found 12 genes up- and 17 significantly down-regulated by BMP-7 (out of 340 investigated genes). Besides the expected activation of anabolic genes chondrocytic cells after BMP-stimulation try to neutralize activation of the BMP-signalling cascade by expressing intra- and extracellular BMP-antagonists. Chondrosarcoma derived cell lines are a potential substitute for primary articular chondrocytes promising consistent expression of a differentiated chondrocyte phenotype with sufficient proliferative capacity. However, as shown by this study one needs to carefully select the cell line depending on the effects which one intends to study. In this respect, HCS-2/8 cells are a validated tool for studying BMP-effects on chondrocytes, but not e.g. effects of interleukin-1.",
keywords = "BMP, Cartilage, Cell Line, Chondrocyte, Chondrosarcoma, DNA array, Growth Factors, IL-1, Microarray",
author = "Joachim Saas and Matthias Gebauer and Carsten Jacobi and Jochen Haag and Masaharu Takigawa and Thomas Aigner",
year = "2005",
language = "English",
volume = "10",
pages = "2027--2035",
journal = "Frontiers in Bioscience - Landmark",
issn = "1093-9946",
publisher = "Frontiers in Bioscience",
number = "SUPPL. 1",

}

TY - JOUR

T1 - The human chondrosarcoma HCS-2/8 cell line is responsive to BMP-7, but not to IL-1beta

AU - Saas, Joachim

AU - Gebauer, Matthias

AU - Jacobi, Carsten

AU - Haag, Jochen

AU - Takigawa, Masaharu

AU - Aigner, Thomas

PY - 2005

Y1 - 2005

N2 - Cultures of primary chondrocytes as in vitro model systems for studying the cellular behavior of chondrocytes are notoriously difficult to cultivate and propagate. One way to circumvent these problems appears to be the use of immortalized/immortal chondrocytic cell lines. In the present study, we were interested whether the chondrosarcoma derived HCS-2/8 cells are suitable for studying major cellular reaction pattern in response to key anabolic (BMP-7) and catabolic (IL-1beta) factors. Therefore, we used cDNA array and real-time PCR technology in order to evaluate gene expression triggerd by stimulation with IL-1beta (0,1-100 ng/ml) and BMP-7 in confluent monolayer cultures. HCS-2/8 cells hardly responded to IL-1beta, but showed good responsiveness to BMP-7. We found 12 genes up- and 17 significantly down-regulated by BMP-7 (out of 340 investigated genes). Besides the expected activation of anabolic genes chondrocytic cells after BMP-stimulation try to neutralize activation of the BMP-signalling cascade by expressing intra- and extracellular BMP-antagonists. Chondrosarcoma derived cell lines are a potential substitute for primary articular chondrocytes promising consistent expression of a differentiated chondrocyte phenotype with sufficient proliferative capacity. However, as shown by this study one needs to carefully select the cell line depending on the effects which one intends to study. In this respect, HCS-2/8 cells are a validated tool for studying BMP-effects on chondrocytes, but not e.g. effects of interleukin-1.

AB - Cultures of primary chondrocytes as in vitro model systems for studying the cellular behavior of chondrocytes are notoriously difficult to cultivate and propagate. One way to circumvent these problems appears to be the use of immortalized/immortal chondrocytic cell lines. In the present study, we were interested whether the chondrosarcoma derived HCS-2/8 cells are suitable for studying major cellular reaction pattern in response to key anabolic (BMP-7) and catabolic (IL-1beta) factors. Therefore, we used cDNA array and real-time PCR technology in order to evaluate gene expression triggerd by stimulation with IL-1beta (0,1-100 ng/ml) and BMP-7 in confluent monolayer cultures. HCS-2/8 cells hardly responded to IL-1beta, but showed good responsiveness to BMP-7. We found 12 genes up- and 17 significantly down-regulated by BMP-7 (out of 340 investigated genes). Besides the expected activation of anabolic genes chondrocytic cells after BMP-stimulation try to neutralize activation of the BMP-signalling cascade by expressing intra- and extracellular BMP-antagonists. Chondrosarcoma derived cell lines are a potential substitute for primary articular chondrocytes promising consistent expression of a differentiated chondrocyte phenotype with sufficient proliferative capacity. However, as shown by this study one needs to carefully select the cell line depending on the effects which one intends to study. In this respect, HCS-2/8 cells are a validated tool for studying BMP-effects on chondrocytes, but not e.g. effects of interleukin-1.

KW - BMP

KW - Cartilage

KW - Cell Line

KW - Chondrocyte

KW - Chondrosarcoma

KW - DNA array

KW - Growth Factors

KW - IL-1

KW - Microarray

UR - http://www.scopus.com/inward/record.url?scp=21344474379&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=21344474379&partnerID=8YFLogxK

M3 - Article

C2 - 15970475

AN - SCOPUS:21344474379

VL - 10

SP - 2027

EP - 2035

JO - Frontiers in Bioscience - Landmark

JF - Frontiers in Bioscience - Landmark

SN - 1093-9946

IS - SUPPL. 1

ER -