Abstract
Genome searches in this study indicate that the nematode Caenorhabditis elegans genome has 2582 bidirectionally oriented genes that account for more than 25% of the total genes. We analyze the transcriptional repression system for one of these predicted bidirectional promoters, which controls the expression of the spermathecal gene sth-1 and collagen gene col-43. These two genes are separated by 1.3 kb and are transcribed bidirectionally. sth-1 is expressed in spermatheca after the L4 stage and col-43 is expressed in the hypodermal cells of the L2d dauer stage. The upstream regions required for the expression of sth-1 and col-43 shared an overlapped control sequence. Two homeoproteins, MAB-18 and CEH-14, were isolated by yeast one-hybrid screening as binding proteins of the overlapped region. MAB-18 bound to two homeodomain-binding sites and interacted with CEH-14 to repress col-43 expression in spermatheca. These results indicate that the two homeoproteins interact with each other to repress col-43 expression in sth-1-expressing tissues. This is the first report of bidirectional gene regulation analysis in the C. elegans genome.
| Original language | English |
|---|---|
| Pages (from-to) | 101-112 |
| Number of pages | 12 |
| Journal | Journal of Molecular Biology |
| Volume | 348 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Apr 22 2005 |
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Keywords
- Bidirectional promoter
- Caenorhabditis briggsae
- Caenorhabditis elegans
- CEH-14
- MAB-18
ASJC Scopus subject areas
- Virology
Cite this
The homeoproteins MAB-18 and CEH-14 insulate the dauer collagen gene col-43 from activation by the adjacent promoter of the spermatheca gene sth-1 in Caenorhabditis elegans. / Bando, Tetsuya; Ikeda, Tatsuji; Kagawa, Hiroaki.
In: Journal of Molecular Biology, Vol. 348, No. 1, 22.04.2005, p. 101-112.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The homeoproteins MAB-18 and CEH-14 insulate the dauer collagen gene col-43 from activation by the adjacent promoter of the spermatheca gene sth-1 in Caenorhabditis elegans
AU - Bando, Tetsuya
AU - Ikeda, Tatsuji
AU - Kagawa, Hiroaki
PY - 2005/4/22
Y1 - 2005/4/22
N2 - Genome searches in this study indicate that the nematode Caenorhabditis elegans genome has 2582 bidirectionally oriented genes that account for more than 25% of the total genes. We analyze the transcriptional repression system for one of these predicted bidirectional promoters, which controls the expression of the spermathecal gene sth-1 and collagen gene col-43. These two genes are separated by 1.3 kb and are transcribed bidirectionally. sth-1 is expressed in spermatheca after the L4 stage and col-43 is expressed in the hypodermal cells of the L2d dauer stage. The upstream regions required for the expression of sth-1 and col-43 shared an overlapped control sequence. Two homeoproteins, MAB-18 and CEH-14, were isolated by yeast one-hybrid screening as binding proteins of the overlapped region. MAB-18 bound to two homeodomain-binding sites and interacted with CEH-14 to repress col-43 expression in spermatheca. These results indicate that the two homeoproteins interact with each other to repress col-43 expression in sth-1-expressing tissues. This is the first report of bidirectional gene regulation analysis in the C. elegans genome.
AB - Genome searches in this study indicate that the nematode Caenorhabditis elegans genome has 2582 bidirectionally oriented genes that account for more than 25% of the total genes. We analyze the transcriptional repression system for one of these predicted bidirectional promoters, which controls the expression of the spermathecal gene sth-1 and collagen gene col-43. These two genes are separated by 1.3 kb and are transcribed bidirectionally. sth-1 is expressed in spermatheca after the L4 stage and col-43 is expressed in the hypodermal cells of the L2d dauer stage. The upstream regions required for the expression of sth-1 and col-43 shared an overlapped control sequence. Two homeoproteins, MAB-18 and CEH-14, were isolated by yeast one-hybrid screening as binding proteins of the overlapped region. MAB-18 bound to two homeodomain-binding sites and interacted with CEH-14 to repress col-43 expression in spermatheca. These results indicate that the two homeoproteins interact with each other to repress col-43 expression in sth-1-expressing tissues. This is the first report of bidirectional gene regulation analysis in the C. elegans genome.
KW - Bidirectional promoter
KW - Caenorhabditis briggsae
KW - Caenorhabditis elegans
KW - CEH-14
KW - MAB-18
UR - http://www.scopus.com/inward/record.url?scp=16244368117&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=16244368117&partnerID=8YFLogxK
U2 - 10.1016/j.jmb.2005.01.045
DO - 10.1016/j.jmb.2005.01.045
M3 - Article
C2 - 15808856
AN - SCOPUS:16244368117
VL - 348
SP - 101
EP - 112
JO - Journal of Molecular Biology
JF - Journal of Molecular Biology
SN - 0022-2836
IS - 1
ER -