The homeodomain protein defective proventriculus is essential for male accessory gland development to enhance fecundity in Drosophila

Ryunosuke Minami, Miyuki Wakabayashi, Seiko Sugimori, Kiichiro Taniguchi, Akihiko Kokuryo, Takao Imano, Takashi Adachi-Yamada, Naoko Watanabe, Hideki Nakagoshi

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

The Drosophila male accessory gland has functions similar to those of the mammalian prostate gland and the seminal vesicle, and secretes accessory gland proteins into the seminal fluid. Each of the two lobes of the accessory gland is composed of two types of binucleate cell: about 1,000 main cells and 40 secondary cells. A well-known accessory gland protein, sex peptide, is secreted from the main cells and induces female postmating response to increase progeny production, whereas little is known about physiological significance of the secondary cells. The homeodomain transcriptional repressor Defective proventriculus (Dve) is strongly expressed in adult secondary cells, and its mutation resulted in loss of secondary cells, mononucleation of main cells, and reduced size of the accessory gland. dve mutant males had low fecundity despite the presence of sex peptide, and failed to induce the female postmating responses of increased egg laying and reduced sexual receptivity. RNAi-mediated dve knockdown males also had low fecundity with normally binucleate main cells. We provide the first evidence that secondary cells are crucial for male fecundity, and also that Dve activity is required for survival of the secondary cells. These findings provide new insights into a mechanism of fertility/fecundity.

Original languageEnglish
Article numbere32302
JournalPLoS One
Volume7
Issue number3
DOIs
Publication statusPublished - Mar 12 2012

Fingerprint

Proventriculus
homeodomain proteins
Homeodomain Proteins
proventriculus
Accessories
Drosophila
Fertility
fecundity
cells
Peptides
Proteins
Cells
peptides
Seminal Vesicles
prostate gland
Fluids
RNA Interference
Cell Size
seminal vesicles
gender

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

The homeodomain protein defective proventriculus is essential for male accessory gland development to enhance fecundity in Drosophila. / Minami, Ryunosuke; Wakabayashi, Miyuki; Sugimori, Seiko; Taniguchi, Kiichiro; Kokuryo, Akihiko; Imano, Takao; Adachi-Yamada, Takashi; Watanabe, Naoko; Nakagoshi, Hideki.

In: PLoS One, Vol. 7, No. 3, e32302, 12.03.2012.

Research output: Contribution to journalArticle

Minami, R, Wakabayashi, M, Sugimori, S, Taniguchi, K, Kokuryo, A, Imano, T, Adachi-Yamada, T, Watanabe, N & Nakagoshi, H 2012, 'The homeodomain protein defective proventriculus is essential for male accessory gland development to enhance fecundity in Drosophila', PLoS One, vol. 7, no. 3, e32302. https://doi.org/10.1371/journal.pone.0032302
Minami, Ryunosuke ; Wakabayashi, Miyuki ; Sugimori, Seiko ; Taniguchi, Kiichiro ; Kokuryo, Akihiko ; Imano, Takao ; Adachi-Yamada, Takashi ; Watanabe, Naoko ; Nakagoshi, Hideki. / The homeodomain protein defective proventriculus is essential for male accessory gland development to enhance fecundity in Drosophila. In: PLoS One. 2012 ; Vol. 7, No. 3.
@article{024314c7b41a4598913cd2818034e8f1,
title = "The homeodomain protein defective proventriculus is essential for male accessory gland development to enhance fecundity in Drosophila",
abstract = "The Drosophila male accessory gland has functions similar to those of the mammalian prostate gland and the seminal vesicle, and secretes accessory gland proteins into the seminal fluid. Each of the two lobes of the accessory gland is composed of two types of binucleate cell: about 1,000 main cells and 40 secondary cells. A well-known accessory gland protein, sex peptide, is secreted from the main cells and induces female postmating response to increase progeny production, whereas little is known about physiological significance of the secondary cells. The homeodomain transcriptional repressor Defective proventriculus (Dve) is strongly expressed in adult secondary cells, and its mutation resulted in loss of secondary cells, mononucleation of main cells, and reduced size of the accessory gland. dve mutant males had low fecundity despite the presence of sex peptide, and failed to induce the female postmating responses of increased egg laying and reduced sexual receptivity. RNAi-mediated dve knockdown males also had low fecundity with normally binucleate main cells. We provide the first evidence that secondary cells are crucial for male fecundity, and also that Dve activity is required for survival of the secondary cells. These findings provide new insights into a mechanism of fertility/fecundity.",
author = "Ryunosuke Minami and Miyuki Wakabayashi and Seiko Sugimori and Kiichiro Taniguchi and Akihiko Kokuryo and Takao Imano and Takashi Adachi-Yamada and Naoko Watanabe and Hideki Nakagoshi",
year = "2012",
month = "3",
day = "12",
doi = "10.1371/journal.pone.0032302",
language = "English",
volume = "7",
journal = "PLoS One",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "3",

}

TY - JOUR

T1 - The homeodomain protein defective proventriculus is essential for male accessory gland development to enhance fecundity in Drosophila

AU - Minami, Ryunosuke

AU - Wakabayashi, Miyuki

AU - Sugimori, Seiko

AU - Taniguchi, Kiichiro

AU - Kokuryo, Akihiko

AU - Imano, Takao

AU - Adachi-Yamada, Takashi

AU - Watanabe, Naoko

AU - Nakagoshi, Hideki

PY - 2012/3/12

Y1 - 2012/3/12

N2 - The Drosophila male accessory gland has functions similar to those of the mammalian prostate gland and the seminal vesicle, and secretes accessory gland proteins into the seminal fluid. Each of the two lobes of the accessory gland is composed of two types of binucleate cell: about 1,000 main cells and 40 secondary cells. A well-known accessory gland protein, sex peptide, is secreted from the main cells and induces female postmating response to increase progeny production, whereas little is known about physiological significance of the secondary cells. The homeodomain transcriptional repressor Defective proventriculus (Dve) is strongly expressed in adult secondary cells, and its mutation resulted in loss of secondary cells, mononucleation of main cells, and reduced size of the accessory gland. dve mutant males had low fecundity despite the presence of sex peptide, and failed to induce the female postmating responses of increased egg laying and reduced sexual receptivity. RNAi-mediated dve knockdown males also had low fecundity with normally binucleate main cells. We provide the first evidence that secondary cells are crucial for male fecundity, and also that Dve activity is required for survival of the secondary cells. These findings provide new insights into a mechanism of fertility/fecundity.

AB - The Drosophila male accessory gland has functions similar to those of the mammalian prostate gland and the seminal vesicle, and secretes accessory gland proteins into the seminal fluid. Each of the two lobes of the accessory gland is composed of two types of binucleate cell: about 1,000 main cells and 40 secondary cells. A well-known accessory gland protein, sex peptide, is secreted from the main cells and induces female postmating response to increase progeny production, whereas little is known about physiological significance of the secondary cells. The homeodomain transcriptional repressor Defective proventriculus (Dve) is strongly expressed in adult secondary cells, and its mutation resulted in loss of secondary cells, mononucleation of main cells, and reduced size of the accessory gland. dve mutant males had low fecundity despite the presence of sex peptide, and failed to induce the female postmating responses of increased egg laying and reduced sexual receptivity. RNAi-mediated dve knockdown males also had low fecundity with normally binucleate main cells. We provide the first evidence that secondary cells are crucial for male fecundity, and also that Dve activity is required for survival of the secondary cells. These findings provide new insights into a mechanism of fertility/fecundity.

UR - http://www.scopus.com/inward/record.url?scp=84858067094&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84858067094&partnerID=8YFLogxK

U2 - 10.1371/journal.pone.0032302

DO - 10.1371/journal.pone.0032302

M3 - Article

C2 - 22427829

AN - SCOPUS:84858067094

VL - 7

JO - PLoS One

JF - PLoS One

SN - 1932-6203

IS - 3

M1 - e32302

ER -