The homeodomain protein defective proventriculus is essential for male accessory gland development to enhance fecundity in Drosophila

Ryunosuke Minami, Miyuki Wakabayashi, Seiko Sugimori, Kiichiro Taniguchi, Akihiko Kokuryo, Takao Imano, Takashi Adachi-Yamada, Naoko Watanabe, Hideki Nakagoshi

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

The Drosophila male accessory gland has functions similar to those of the mammalian prostate gland and the seminal vesicle, and secretes accessory gland proteins into the seminal fluid. Each of the two lobes of the accessory gland is composed of two types of binucleate cell: about 1,000 main cells and 40 secondary cells. A well-known accessory gland protein, sex peptide, is secreted from the main cells and induces female postmating response to increase progeny production, whereas little is known about physiological significance of the secondary cells. The homeodomain transcriptional repressor Defective proventriculus (Dve) is strongly expressed in adult secondary cells, and its mutation resulted in loss of secondary cells, mononucleation of main cells, and reduced size of the accessory gland. dve mutant males had low fecundity despite the presence of sex peptide, and failed to induce the female postmating responses of increased egg laying and reduced sexual receptivity. RNAi-mediated dve knockdown males also had low fecundity with normally binucleate main cells. We provide the first evidence that secondary cells are crucial for male fecundity, and also that Dve activity is required for survival of the secondary cells. These findings provide new insights into a mechanism of fertility/fecundity.

Original languageEnglish
Article numbere32302
JournalPloS one
Volume7
Issue number3
DOIs
Publication statusPublished - Mar 12 2012

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

Fingerprint Dive into the research topics of 'The homeodomain protein defective proventriculus is essential for male accessory gland development to enhance fecundity in Drosophila'. Together they form a unique fingerprint.

  • Cite this

    Minami, R., Wakabayashi, M., Sugimori, S., Taniguchi, K., Kokuryo, A., Imano, T., Adachi-Yamada, T., Watanabe, N., & Nakagoshi, H. (2012). The homeodomain protein defective proventriculus is essential for male accessory gland development to enhance fecundity in Drosophila. PloS one, 7(3), [e32302]. https://doi.org/10.1371/journal.pone.0032302