The helper component-proteinase of Sweet potato feathery mottle virus facilitates systemic spread of Potato virus X in Ipomoea nil

S. Sonoda, H. Koiwa, K. Kanda, H. Kato, M. Shimono, M. Nishiguchi

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

When Ipomoea nil was coinfected with Sweet potato feathery mottle virus (SPFMV), a member of the genus Potyvirus, and Potato virus X (PVX) typical symptoms caused by PVX were observed on those by SPFMV on the first upper tree leaves at 14 days postinoculation (dpi). On the other hand, no PVX-induced symptoms were observed on the first upper true leaves at 14 dpi when plants were infected with PVX alone. In the case of coinfection with PVX and SPFMV, PVX RNA was detected not only in the inoculated cotyledonary leaves but also in the first upper true leaves at 14 dpi. In the case of single infection with PVX, PVX RNA was detected in the inoculated cotyledonary leaves but not in the first upper true leaves at 14 dpi. The accumulation of SPFMV remained unchanged, regardless of whether the inoculum consisted of SPFMV alone or a mixture of SPFMV and PVX. Although recombinant PVX engineered to express the helper component-proteinase (HC-Pro) of SPFMV (PVX.HC) enhanced symptoms severity in Nicotiana benthamiana, PVX.HC induced the synergism characterized by an enhanced viral movement in Ipomoea nil. Immunofluorescence microscopic examination revealed that the HC-Pro was present in phloem of SPFMV-infected I. nil. These results suggest that SPFMV HC-Pro acts as an enhancer of long distance movement for PVX in I. nil.

Original languageEnglish
Pages (from-to)944-950
Number of pages7
JournalPhytopathology
Volume90
Issue number9
Publication statusPublished - 2000
Externally publishedYes

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Sweet potato feathery mottle virus
Ipomoea nil
Potato virus X
proteinases
signs and symptoms (plants)
leaves
RNA
Potyvirus
Nicotiana benthamiana
synergism
mixed infection

ASJC Scopus subject areas

  • Plant Science

Cite this

Sonoda, S., Koiwa, H., Kanda, K., Kato, H., Shimono, M., & Nishiguchi, M. (2000). The helper component-proteinase of Sweet potato feathery mottle virus facilitates systemic spread of Potato virus X in Ipomoea nil. Phytopathology, 90(9), 944-950.

The helper component-proteinase of Sweet potato feathery mottle virus facilitates systemic spread of Potato virus X in Ipomoea nil. / Sonoda, S.; Koiwa, H.; Kanda, K.; Kato, H.; Shimono, M.; Nishiguchi, M.

In: Phytopathology, Vol. 90, No. 9, 2000, p. 944-950.

Research output: Contribution to journalArticle

Sonoda, S, Koiwa, H, Kanda, K, Kato, H, Shimono, M & Nishiguchi, M 2000, 'The helper component-proteinase of Sweet potato feathery mottle virus facilitates systemic spread of Potato virus X in Ipomoea nil', Phytopathology, vol. 90, no. 9, pp. 944-950.
Sonoda, S. ; Koiwa, H. ; Kanda, K. ; Kato, H. ; Shimono, M. ; Nishiguchi, M. / The helper component-proteinase of Sweet potato feathery mottle virus facilitates systemic spread of Potato virus X in Ipomoea nil. In: Phytopathology. 2000 ; Vol. 90, No. 9. pp. 944-950.
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