The GYF domain protein PSIG1 dampens the induction of cell death during plant-pathogen interactions

Hidenori Matsui, Yuko Nomura, Mayumi Egusa, Takahiro Hamada, Gang Su Hyon, Hironori Kaminaka, Yuichiro Watanabe, Takashi Ueda, Marco Trujillo, Ken Shirasu, Hirofumi Nakagami

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The induction of rapid cell death is an effective strategy for plants to restrict biotrophic and hemi-biotrophic pathogens at the infection site. However, activation of cell death comes at a high cost, as dead cells will no longer be available for defense responses nor general metabolic processes. In addition, necrotrophic pathogens that thrive on dead tissue, take advantage of cell death-triggering mechanisms. Mechanisms by which plants solve this conundrum remain described. Here, we identify PLANT SMY2-TYPE ILE-GYF DOMAIN-CONTAINING PROTEIN 1 (PSIG1) and show that PSIG1 helps to restrict cell death induction during pathogen infection. Inactivation of PSIG1 does not result in spontaneous lesions, and enhanced cell death in psig1 mutants is independent of salicylic acid (SA) biosynthesis or reactive oxygen species (ROS) production. Moreover, PSIG1 interacts with SMG7, which plays a role in nonsense-mediated RNA decay (NMD), and the smg7-4 mutant allele mimics the cell death phenotype of the psig1 mutants. Intriguingly, the psig1 mutants display enhanced susceptibility to the hemi-biotrophic bacterial pathogen. These findings point to the existence and importance of the SA- and ROS-independent cell death constraining mechanism as a part of the plant immune system.

Original languageEnglish
Article numbere1007037
JournalPLoS Genetics
Volume13
Issue number10
DOIs
Publication statusPublished - Oct 1 2017

Fingerprint

plant pathogens
cell death
Cell Death
pathogen
salicylic acid
protein
proteins
mutants
Salicylic Acid
pathogens
immune system
reactive oxygen species
Reactive Oxygen Species
lesion
phenotype
RNA
Plant Immunity
allele
lesions (plant)
RNA Stability

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Molecular Biology
  • Genetics
  • Genetics(clinical)
  • Cancer Research

Cite this

Matsui, H., Nomura, Y., Egusa, M., Hamada, T., Hyon, G. S., Kaminaka, H., ... Nakagami, H. (2017). The GYF domain protein PSIG1 dampens the induction of cell death during plant-pathogen interactions. PLoS Genetics, 13(10), [e1007037]. https://doi.org/10.1371/journal.pgen.1007037

The GYF domain protein PSIG1 dampens the induction of cell death during plant-pathogen interactions. / Matsui, Hidenori; Nomura, Yuko; Egusa, Mayumi; Hamada, Takahiro; Hyon, Gang Su; Kaminaka, Hironori; Watanabe, Yuichiro; Ueda, Takashi; Trujillo, Marco; Shirasu, Ken; Nakagami, Hirofumi.

In: PLoS Genetics, Vol. 13, No. 10, e1007037, 01.10.2017.

Research output: Contribution to journalArticle

Matsui, H, Nomura, Y, Egusa, M, Hamada, T, Hyon, GS, Kaminaka, H, Watanabe, Y, Ueda, T, Trujillo, M, Shirasu, K & Nakagami, H 2017, 'The GYF domain protein PSIG1 dampens the induction of cell death during plant-pathogen interactions', PLoS Genetics, vol. 13, no. 10, e1007037. https://doi.org/10.1371/journal.pgen.1007037
Matsui, Hidenori ; Nomura, Yuko ; Egusa, Mayumi ; Hamada, Takahiro ; Hyon, Gang Su ; Kaminaka, Hironori ; Watanabe, Yuichiro ; Ueda, Takashi ; Trujillo, Marco ; Shirasu, Ken ; Nakagami, Hirofumi. / The GYF domain protein PSIG1 dampens the induction of cell death during plant-pathogen interactions. In: PLoS Genetics. 2017 ; Vol. 13, No. 10.
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