TY - JOUR
T1 - The guanosine binding mechanism of the Tetrahymena group I intron.
AU - Kitamura, A.
AU - Muto, Y.
AU - Watanabe, S.
AU - Kim, I.
AU - Ito, T.
AU - Nishiya, Y.
AU - Ohtsuki, T.
AU - Kawai, G.
AU - Watanabe, K.
AU - Hosono, K.
AU - Takaku, H.
AU - Katoh, E.
AU - Yamazaki, T.
AU - Inoue, T.
AU - Yokoyama, S.
PY - 1999
Y1 - 1999
N2 - The Tetrahymena group I intron catalyzes self-splicing through two consecutive transesterification reactions, using a single guanosine-binding site (GBS). In this study, we constructed a model RNA that contains the GBS and a conserved guanosine nucleotide at the 3'-terminus of the intron (omegaG). We determined by NMR the solution structure of this model RNA, and revealed the guanosine binding mechanism of the group I intron. The G22 residue, corresponding to omegaG, participates in a base triple, G22 xx G3 x C12, hydrogen-bonding to the major groove edge of the Watson-Crick G3 x C12 pair. The G22 residue also interacts with A2, which is semi-conserved in all sequenced group I introns.
AB - The Tetrahymena group I intron catalyzes self-splicing through two consecutive transesterification reactions, using a single guanosine-binding site (GBS). In this study, we constructed a model RNA that contains the GBS and a conserved guanosine nucleotide at the 3'-terminus of the intron (omegaG). We determined by NMR the solution structure of this model RNA, and revealed the guanosine binding mechanism of the group I intron. The G22 residue, corresponding to omegaG, participates in a base triple, G22 xx G3 x C12, hydrogen-bonding to the major groove edge of the Watson-Crick G3 x C12 pair. The G22 residue also interacts with A2, which is semi-conserved in all sequenced group I introns.
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U2 - 10.1093/nass/42.1.191
DO - 10.1093/nass/42.1.191
M3 - Article
C2 - 10780444
AN - SCOPUS:0033288758
SN - 0261-3166
SP - 191
EP - 192
JO - Nucleic acids symposium series
JF - Nucleic acids symposium series
IS - 42
ER -