The group E Sox genes Sox8 and Sox9 are regulated by Notch signaling and are required for Müller glial cell development in mouse retina

Akihiko Muto, Atsumi Iida, Shinya Satoh, Sumiko Watanabe

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

Although Müller glial cells play pivotal roles in the vertebrate retina, the regulation of their development is poorly understood. While Notch-Hes5 signaling has been shown to be involved in this developmental process, the presence of Müller glial cells in Hes5-deficient mice suggests the involvement of other molecules. We found that two group E Sox genes, Sox8 and Sox9, are expressed in proliferating progenitors and then exclusively in Müller glial cells in mouse retina. Knocking-down Sox8 and Sox9 by shRNA significantly reduced the population of Müller glial cells and relatively increased the proportion of rod photoreceptors, suggesting that the Sox genes play roles in the specification of Müller glial cells. Using an activated form of Notch and the γ-secretase inhibitor DAPT, we also found that Notch signaling regulates the transcription of Sox8 and Sox9. This is the first evidence that group E Sox genes play important roles in the developing vertebrate retina.

Original languageEnglish
Pages (from-to)549-558
Number of pages10
JournalExperimental Eye Research
Volume89
Issue number4
DOIs
Publication statusPublished - Oct 2009
Externally publishedYes

Fingerprint

Neuroglia
Retina
Genes
Vertebrates
Retinal Rod Photoreceptor Cells
Amyloid Precursor Protein Secretases
Small Interfering RNA
Population

Keywords

  • development
  • mouse
  • Müller glial cell
  • notch
  • retina
  • Sox8
  • Sox9
  • specification

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

Cite this

The group E Sox genes Sox8 and Sox9 are regulated by Notch signaling and are required for Müller glial cell development in mouse retina. / Muto, Akihiko; Iida, Atsumi; Satoh, Shinya; Watanabe, Sumiko.

In: Experimental Eye Research, Vol. 89, No. 4, 10.2009, p. 549-558.

Research output: Contribution to journalArticle

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