The glycerophosphoryl diester phosphodiesterase-like proteins SHV3 and its homologs play important roles in cell wall organization

Shimpei Hayashi, Tadashi Ishii, Toshiro Matsunaga, Rumi Tominaga, Takashi Kuromori, Takuji Wada, Kazuo Shinozaki, Takashi Hirayama

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

Despite the importance of extracellular events in cell wall organization and biogenesis, the mechanisms and related factors are largely unknown. We isolated an allele of the shaven3 (shv3) mutant of Arabidopsis thaliana, which exhibits ruptured root hair cells during tip growth. SHV3 encodes a novel protein with two tandemly repeated glycerophosphoryl diester phosphodiesterase-like domains and a glycosylphosphatidylinositol anchor, and several of its paralogs are found in Arabidopsis. Here, we report the detailed characterization of mutants of SHV3 and one of its paralogs, SVL1. The shv3 and svl1 double mutant exhibited additional defects, including swollen guard cells, aberrant expansion of the hypocotyl epidermis and ectopic lignin deposits, suggesting decreased rigidity of the cell wall. Fourier-transform infrared spectroscopy and measurement of the cell wall components indicated an altered cellulose content and pectin modification with cross-linking in the double mutant. Furthermore, we found that the ruptured root hair phenotype of shv3 was suppressed by increasing the amount of borate, which is supposed to be involved in pectic polysaccharide cross-linking, in the medium. These findings indicate that SHV3 and its paralogs are novel important factors involved in primary cell wall organization.

Original languageEnglish
Pages (from-to)1522-1535
Number of pages14
JournalPlant and Cell Physiology
Volume49
Issue number10
DOIs
Publication statusPublished - Oct 2008
Externally publishedYes

Fingerprint

Phosphoric Diester Hydrolases
Cell Wall
cell walls
mutants
root hairs
crosslinking
Arabidopsis
Proteins
proteins
Hypocotyl
Glycosylphosphatidylinositols
borates
Borates
Lignin
guard cells
Fourier transform infrared spectroscopy
cell wall components
Fourier Transform Infrared Spectroscopy
Cellular Structures
epidermis (plant)

Keywords

  • Arabidopsis thaliana
  • Cell wall
  • Cellulose
  • GPI-anchored protein
  • Pectin
  • Root hair

ASJC Scopus subject areas

  • Plant Science
  • Physiology
  • Cell Biology

Cite this

The glycerophosphoryl diester phosphodiesterase-like proteins SHV3 and its homologs play important roles in cell wall organization. / Hayashi, Shimpei; Ishii, Tadashi; Matsunaga, Toshiro; Tominaga, Rumi; Kuromori, Takashi; Wada, Takuji; Shinozaki, Kazuo; Hirayama, Takashi.

In: Plant and Cell Physiology, Vol. 49, No. 10, 10.2008, p. 1522-1535.

Research output: Contribution to journalArticle

Hayashi, Shimpei ; Ishii, Tadashi ; Matsunaga, Toshiro ; Tominaga, Rumi ; Kuromori, Takashi ; Wada, Takuji ; Shinozaki, Kazuo ; Hirayama, Takashi. / The glycerophosphoryl diester phosphodiesterase-like proteins SHV3 and its homologs play important roles in cell wall organization. In: Plant and Cell Physiology. 2008 ; Vol. 49, No. 10. pp. 1522-1535.
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