Abstract
The global regulator LaeA is required for the expression of biosynthetic genes for secondary metabolites in filamentous fungi such as Aspergillus nidulans. To learn more about the regulation of these genes in Alternaria alternata, which has at least seven pathogenic variants (pathotypes) that produce host-specific toxins (HSTs) and cause severe diseases in their host plants, we identified LaeA homologs encoding methyltransferase in tomato, strawberry and apple pathotypes of A. alternata, designating them AtLAE1, AsLAE1 and AaLAE1, respectively. In the AtLAE1-deleted mutant of the tomato pathotype (∆AtLAE1), expression of the AAL-toxin biosynthetic gene ALT1 was lower than that in the wild type. Correspondingly, AAL-toxin production and virulence of the mutant were significantly lower as were spore production and hyphal growth. Similarly, the AsLAE1-deleted mutant of the strawberry pathotype (∆AsLAE1) and the AaLAE1-deleted mutant of the apple pathotype (∆AaLAE1) produced less of their host-specific toxin (AF- and AM-toxin, respectively), had reduced virulence on the host plant, and hyphal growth and sporulation were defective. Thus, the global regulator gene LaeA positively regulates HST biosynthesis, pathogenicity, growth and differentiation in A. alternata pathotypes.
| Original language | English |
|---|---|
| Pages (from-to) | 1-11 |
| Number of pages | 11 |
| Journal | Journal of General Plant Pathology |
| DOIs | |
| Publication status | Accepted/In press - Mar 16 2016 |
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Keywords
- Alternaria alternata pathotypes
- Global regulator
- Host-specific toxin
- LaeA
- Secondary metabolite
ASJC Scopus subject areas
- Plant Science
- Agronomy and Crop Science
Cite this
The global regulator LaeA controls biosynthesis of host-specific toxins, pathogenicity and development of Alternaria alternata pathotypes. / Takao, Kazumi; Akagi, Yasunori; Tsuge, Takashi; Harimoto, Yoshiaki; Yamamoto, Mikihiro; Kodama, Motoichiro.
In: Journal of General Plant Pathology, 16.03.2016, p. 1-11.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The global regulator LaeA controls biosynthesis of host-specific toxins, pathogenicity and development of Alternaria alternata pathotypes
AU - Takao, Kazumi
AU - Akagi, Yasunori
AU - Tsuge, Takashi
AU - Harimoto, Yoshiaki
AU - Yamamoto, Mikihiro
AU - Kodama, Motoichiro
PY - 2016/3/16
Y1 - 2016/3/16
N2 - The global regulator LaeA is required for the expression of biosynthetic genes for secondary metabolites in filamentous fungi such as Aspergillus nidulans. To learn more about the regulation of these genes in Alternaria alternata, which has at least seven pathogenic variants (pathotypes) that produce host-specific toxins (HSTs) and cause severe diseases in their host plants, we identified LaeA homologs encoding methyltransferase in tomato, strawberry and apple pathotypes of A. alternata, designating them AtLAE1, AsLAE1 and AaLAE1, respectively. In the AtLAE1-deleted mutant of the tomato pathotype (∆AtLAE1), expression of the AAL-toxin biosynthetic gene ALT1 was lower than that in the wild type. Correspondingly, AAL-toxin production and virulence of the mutant were significantly lower as were spore production and hyphal growth. Similarly, the AsLAE1-deleted mutant of the strawberry pathotype (∆AsLAE1) and the AaLAE1-deleted mutant of the apple pathotype (∆AaLAE1) produced less of their host-specific toxin (AF- and AM-toxin, respectively), had reduced virulence on the host plant, and hyphal growth and sporulation were defective. Thus, the global regulator gene LaeA positively regulates HST biosynthesis, pathogenicity, growth and differentiation in A. alternata pathotypes.
AB - The global regulator LaeA is required for the expression of biosynthetic genes for secondary metabolites in filamentous fungi such as Aspergillus nidulans. To learn more about the regulation of these genes in Alternaria alternata, which has at least seven pathogenic variants (pathotypes) that produce host-specific toxins (HSTs) and cause severe diseases in their host plants, we identified LaeA homologs encoding methyltransferase in tomato, strawberry and apple pathotypes of A. alternata, designating them AtLAE1, AsLAE1 and AaLAE1, respectively. In the AtLAE1-deleted mutant of the tomato pathotype (∆AtLAE1), expression of the AAL-toxin biosynthetic gene ALT1 was lower than that in the wild type. Correspondingly, AAL-toxin production and virulence of the mutant were significantly lower as were spore production and hyphal growth. Similarly, the AsLAE1-deleted mutant of the strawberry pathotype (∆AsLAE1) and the AaLAE1-deleted mutant of the apple pathotype (∆AaLAE1) produced less of their host-specific toxin (AF- and AM-toxin, respectively), had reduced virulence on the host plant, and hyphal growth and sporulation were defective. Thus, the global regulator gene LaeA positively regulates HST biosynthesis, pathogenicity, growth and differentiation in A. alternata pathotypes.
KW - Alternaria alternata pathotypes
KW - Global regulator
KW - Host-specific toxin
KW - LaeA
KW - Secondary metabolite
UR - http://www.scopus.com/inward/record.url?scp=84961204446&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84961204446&partnerID=8YFLogxK
U2 - 10.1007/s10327-016-0656-9
DO - 10.1007/s10327-016-0656-9
M3 - Article
AN - SCOPUS:84961204446
SP - 1
EP - 11
JO - Journal of General Plant Pathology
JF - Journal of General Plant Pathology
SN - 1345-2630
ER -