The global regulator LaeA controls biosynthesis of host-specific toxins, pathogenicity and development of Alternaria alternata pathotypes

Kazumi Takao, Yasunori Akagi, Takashi Tsuge, Yoshiaki Harimoto, Mikihiro Yamamoto, Motoichiro Kodama

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The global regulator LaeA is required for the expression of biosynthetic genes for secondary metabolites in filamentous fungi such as Aspergillus nidulans. To learn more about the regulation of these genes in Alternaria alternata, which has at least seven pathogenic variants (pathotypes) that produce host-specific toxins (HSTs) and cause severe diseases in their host plants, we identified LaeA homologs encoding methyltransferase in tomato, strawberry and apple pathotypes of A. alternata, designating them AtLAE1, AsLAE1 and AaLAE1, respectively. In the AtLAE1-deleted mutant of the tomato pathotype (∆AtLAE1), expression of the AAL-toxin biosynthetic gene ALT1 was lower than that in the wild type. Correspondingly, AAL-toxin production and virulence of the mutant were significantly lower as were spore production and hyphal growth. Similarly, the AsLAE1-deleted mutant of the strawberry pathotype (∆AsLAE1) and the AaLAE1-deleted mutant of the apple pathotype (∆AaLAE1) produced less of their host-specific toxin (AF- and AM-toxin, respectively), had reduced virulence on the host plant, and hyphal growth and sporulation were defective. Thus, the global regulator gene LaeA positively regulates HST biosynthesis, pathogenicity, growth and differentiation in A. alternata pathotypes.

Original languageEnglish
Pages (from-to)1-11
Number of pages11
JournalJournal of General Plant Pathology
DOIs
Publication statusAccepted/In press - Mar 16 2016

Fingerprint

Alternaria alternata
pathotypes
toxins
pathogenicity
biosynthesis
mutants
strawberries
virulence
host plants
apples
tomatoes
Aspergillus nidulans
genes
methyltransferases
regulator genes
sporulation
secondary metabolites
spores
fungi

Keywords

  • Alternaria alternata pathotypes
  • Global regulator
  • Host-specific toxin
  • LaeA
  • Secondary metabolite

ASJC Scopus subject areas

  • Plant Science
  • Agronomy and Crop Science

Cite this

The global regulator LaeA controls biosynthesis of host-specific toxins, pathogenicity and development of Alternaria alternata pathotypes. / Takao, Kazumi; Akagi, Yasunori; Tsuge, Takashi; Harimoto, Yoshiaki; Yamamoto, Mikihiro; Kodama, Motoichiro.

In: Journal of General Plant Pathology, 16.03.2016, p. 1-11.

Research output: Contribution to journalArticle

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