The genotoxicity of UVA irradiation in Drosophila melanogaster and the synergistic action of 8-methoxypsoralen and UVA

Tomoe Negishi, Fujimi Tanabe, Hikoya Hayatsu

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

To study the genotoxicity of near-ultraviolet light (UVA) on a whole body, Drosophila melanogaster larvae were irradiated with UVA and the emerging flies were examined for the mutant wing spot formation. The genotoxicity of UVA was also assayed with the in vivo DNA-repair test using males with repair-deficiency at the mei-9 and mei-41 locus and the matching repair-proficient females. Third-instar larvae were placed in a plastic Petri dish, which was covered with soft glass, and irradiated with black light at 4-5 W/m2. This irradiation resulted in an increase in mutant wing-hair spots. After a 15 h irradiation (∼240 kJ/m2), the mutant clone frequencies found in the adult flies (spots/wing) were: 1.68 for the small single spots, 0.38 for the large single spots and 0.11 for the twin spots, while at zero time they were 0.68, 0.06 and 0.02 respectively. On the other hand, the UVA irradiation was negative in the in vivo DNA-repair test, indicating that the UVA-induced DNA lesion may not be subject to repair by the mei-9 and mei-41 functions. The presence of 8-methoxypsoralen (8-MOP) during the irradiation remarkably enhanced somatic mutations, and showed a strong DNA-damaging effect in the repair test. For example, a 15 h UVA irradiation with 26.7 μM 8-MOP resulted in a 14-fold increase in the number of twin spots per wing as compared with the frequency obtained on treatment with UVA alone. Treatment of the larvae with 8-MOP alone gave no mutant clones or DNA damage. A high frequency in twin spot formation was also observed in this UVA + 8-MOP treatment, indicating that extensive chromosomal recombinations took place in the somatic cells.

Original languageEnglish
Pages (from-to)1433-1436
Number of pages4
JournalCarcinogenesis
Volume13
Issue number8
Publication statusPublished - Aug 1992

Fingerprint

Methoxsalen
Drosophilidae
Drosophila melanogaster
Irradiation
Repair
DNA
Larva
Mutant
Diptera
DNA Repair
Clone Cells
Clone
Ultraviolet Rays
Genetic Recombination
Plastics
DNA Damage
Glass
Light
Recombination
Ultraviolet

ASJC Scopus subject areas

  • Cancer Research
  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Physiology (medical)
  • Physiology
  • Behavioral Neuroscience

Cite this

The genotoxicity of UVA irradiation in Drosophila melanogaster and the synergistic action of 8-methoxypsoralen and UVA. / Negishi, Tomoe; Tanabe, Fujimi; Hayatsu, Hikoya.

In: Carcinogenesis, Vol. 13, No. 8, 08.1992, p. 1433-1436.

Research output: Contribution to journalArticle

Negishi, Tomoe ; Tanabe, Fujimi ; Hayatsu, Hikoya. / The genotoxicity of UVA irradiation in Drosophila melanogaster and the synergistic action of 8-methoxypsoralen and UVA. In: Carcinogenesis. 1992 ; Vol. 13, No. 8. pp. 1433-1436.
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