Abstract
The genotoxicities of a series of N-nitrosamines were assayed in the wing spot test and a new short-term test of Drosophila melanogaster. In the spot test, larval flies trans-heterozygous for the somatic cell markers mwh and flr3 were fed the test reagents and the wing hairs in adults were inspected for clones expressing the phenotypes of the markers. In the other test, larval stock consisting of meiotic recombination-deficient (Rec-) double mutant mei-9a and mei-41D5 males and repair-proficient Rec+ females were grown on feed containing the reagents and the DNA damages were detected with the preferential killing of the Rec- larvae as an endpoint. The carcinogenic nitrosamines tested, N-nitrosodimethylamine (NDMA), N-nitrosodiethylamine (NDEA), N-nitrosodi-n-butylamine (NDBA), N-nitrosomorpholine (NMOR), N-nitrosopiperidine (NPIP) and N-nitrosopyrrolidine (NPYR), all showed clearly positive activities in both tests. The activities in the wing spot test were ranked in a sequence of NDMA ≫ NMOR > NPIP > NDEA NPYR > NDBA. A similar ranking was obtained in the repair assay. The genotoxicity of N-nitrosodiphyenylamine (NDPhA), carcinogenicity studies of which are inconclusive, was marginal in the spot test. The non-carcinogenic N-nitrosoproline (NPRO) and the non-mutagenic N-nitrosothioproline (NTPRO) were negative in the spot test. NDPhA and NPRO were negative in the repair test as well. The DNA-repair test is thus a convenient technique for estimating the mutagenicity of compounds because of its simplicity compared with the wing spot test. These Drosophila tests may be useful in predicting carcinogenic potentials of compounds.
| Original language | English |
|---|---|
| Pages (from-to) | 119-128 |
| Number of pages | 10 |
| Journal | Mutation Research/Environmental Mutagenesis and Related Subjects |
| Volume | 252 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 1991 |
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Keywords
- DNA-repair test
- Drosophila melanogaster
- N-Nitrosamines
- Wing spot test
ASJC Scopus subject areas
- Genetics
- Toxicology
- Medicine(all)
Cite this
The genotoxicities of N-nitrosamines in Drosophila melanogaster in vivo : the correlation of mutagenicity in the wing spot test with the DNA damages detected by the DNA-repair test. / Negishi, Tomoe; Shiotani, Teruko; Fujikawa, Kazuo; Hayatsu, Hikoya.
In: Mutation Research/Environmental Mutagenesis and Related Subjects, Vol. 252, No. 2, 1991, p. 119-128.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The genotoxicities of N-nitrosamines in Drosophila melanogaster in vivo
T2 - the correlation of mutagenicity in the wing spot test with the DNA damages detected by the DNA-repair test
AU - Negishi, Tomoe
AU - Shiotani, Teruko
AU - Fujikawa, Kazuo
AU - Hayatsu, Hikoya
PY - 1991
Y1 - 1991
N2 - The genotoxicities of a series of N-nitrosamines were assayed in the wing spot test and a new short-term test of Drosophila melanogaster. In the spot test, larval flies trans-heterozygous for the somatic cell markers mwh and flr3 were fed the test reagents and the wing hairs in adults were inspected for clones expressing the phenotypes of the markers. In the other test, larval stock consisting of meiotic recombination-deficient (Rec-) double mutant mei-9a and mei-41D5 males and repair-proficient Rec+ females were grown on feed containing the reagents and the DNA damages were detected with the preferential killing of the Rec- larvae as an endpoint. The carcinogenic nitrosamines tested, N-nitrosodimethylamine (NDMA), N-nitrosodiethylamine (NDEA), N-nitrosodi-n-butylamine (NDBA), N-nitrosomorpholine (NMOR), N-nitrosopiperidine (NPIP) and N-nitrosopyrrolidine (NPYR), all showed clearly positive activities in both tests. The activities in the wing spot test were ranked in a sequence of NDMA ≫ NMOR > NPIP > NDEA NPYR > NDBA. A similar ranking was obtained in the repair assay. The genotoxicity of N-nitrosodiphyenylamine (NDPhA), carcinogenicity studies of which are inconclusive, was marginal in the spot test. The non-carcinogenic N-nitrosoproline (NPRO) and the non-mutagenic N-nitrosothioproline (NTPRO) were negative in the spot test. NDPhA and NPRO were negative in the repair test as well. The DNA-repair test is thus a convenient technique for estimating the mutagenicity of compounds because of its simplicity compared with the wing spot test. These Drosophila tests may be useful in predicting carcinogenic potentials of compounds.
AB - The genotoxicities of a series of N-nitrosamines were assayed in the wing spot test and a new short-term test of Drosophila melanogaster. In the spot test, larval flies trans-heterozygous for the somatic cell markers mwh and flr3 were fed the test reagents and the wing hairs in adults were inspected for clones expressing the phenotypes of the markers. In the other test, larval stock consisting of meiotic recombination-deficient (Rec-) double mutant mei-9a and mei-41D5 males and repair-proficient Rec+ females were grown on feed containing the reagents and the DNA damages were detected with the preferential killing of the Rec- larvae as an endpoint. The carcinogenic nitrosamines tested, N-nitrosodimethylamine (NDMA), N-nitrosodiethylamine (NDEA), N-nitrosodi-n-butylamine (NDBA), N-nitrosomorpholine (NMOR), N-nitrosopiperidine (NPIP) and N-nitrosopyrrolidine (NPYR), all showed clearly positive activities in both tests. The activities in the wing spot test were ranked in a sequence of NDMA ≫ NMOR > NPIP > NDEA NPYR > NDBA. A similar ranking was obtained in the repair assay. The genotoxicity of N-nitrosodiphyenylamine (NDPhA), carcinogenicity studies of which are inconclusive, was marginal in the spot test. The non-carcinogenic N-nitrosoproline (NPRO) and the non-mutagenic N-nitrosothioproline (NTPRO) were negative in the spot test. NDPhA and NPRO were negative in the repair test as well. The DNA-repair test is thus a convenient technique for estimating the mutagenicity of compounds because of its simplicity compared with the wing spot test. These Drosophila tests may be useful in predicting carcinogenic potentials of compounds.
KW - DNA-repair test
KW - Drosophila melanogaster
KW - N-Nitrosamines
KW - Wing spot test
UR - http://www.scopus.com/inward/record.url?scp=0026082052&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0026082052&partnerID=8YFLogxK
U2 - 10.1016/0165-1161(91)90012-W
DO - 10.1016/0165-1161(91)90012-W
M3 - Article
C2 - 1901957
AN - SCOPUS:0026082052
VL - 252
SP - 119
EP - 128
JO - Mutation Research - Environmental Mutagenesis and Related Subjects Including Methodology
JF - Mutation Research - Environmental Mutagenesis and Related Subjects Including Methodology
SN - 0165-1161
IS - 2
ER -