The genetic diversity of Helicobacter pylori virulence genes is not associated with gastric atrophy progression

Masahide Kita, Kenji Yokota, Hiroyuki Okada, Susumu Take, Ryuta Takenaka, Yoshiro Kawahara, Keiji Oguma, Osamu Matsushita, Kazuhide Yamamoto

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Atrophy of the gastric mucosa is a precursor of intestinal-type gastric cancer, and Helicobacter pylori infection causes atrophic gastritis. The aim of this study was to determine whether the genetic diversity of H. pylori virulence genes is associated with the development and progression of gastric atrophy in humans. We isolated and cultured H. pylori strains from patients with gastric ulcer and duodenal ulcer accompanied by atrophic gastritis in background mucosa. H. pylori strains were stored at - 80°C prior to the experiments being carried out. We analyzed iceA, babA, vacA, cagA, and cagE genes by PCR. The cagA gene was analyzed through sequencing of the C-terminal region containing the EPIYA motif, which is related to tyrosine phosphorylation. Severe atrophy was observed in patients with gastric ulcer. The major phenotype of the vacA gene was slc/ml (93%). The cagA gene was detected in all strains. The cagE gene was not detected in 2 and 5 strains from the mild cases and severe cases, respectively. The major cagA EPIYA motif, which is amino acids repeat in the C terminus, was the A-B-D type (44 of 58 strains). The virulence genes were not statistically associated with the severity of atrophy in the background gastric mucosa in humans. Not only identification of bacterial virulence factors but also studies of the host response will be necessary to investigate the progression of gastric atrophy and subsequent cancer development in humans.

Original languageEnglish
Pages (from-to)93-98
Number of pages6
JournalActa Medica Okayama
Volume67
Issue number2
Publication statusPublished - 2013

Fingerprint

Helicobacter pylori
Atrophy
Virulence
Stomach
Genes
Atrophic Gastritis
Stomach Ulcer
Gastric Mucosa
Intestinal Neoplasms
Phosphorylation
Human Development
Helicobacter Infections
Virulence Factors
Duodenal Ulcer
Stomach Neoplasms
Tyrosine
Mucous Membrane
Phenotype
Amino Acids
Polymerase Chain Reaction

Keywords

  • Chronic atrophic gastritis
  • Helicobacter pylori
  • Virulence genes

ASJC Scopus subject areas

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

The genetic diversity of Helicobacter pylori virulence genes is not associated with gastric atrophy progression. / Kita, Masahide; Yokota, Kenji; Okada, Hiroyuki; Take, Susumu; Takenaka, Ryuta; Kawahara, Yoshiro; Oguma, Keiji; Matsushita, Osamu; Yamamoto, Kazuhide.

In: Acta Medica Okayama, Vol. 67, No. 2, 2013, p. 93-98.

Research output: Contribution to journalArticle

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