The FtsH protease heterocomplex in Arabidopsis: Dispensability of Type-B protease activity for proper chloroplast development

Di Zhang, Yusuke Kato, Lingang Zhang, Masaru Fujimoto, Nobuhiro Tsutsumi, S. Sodmergen, Wataru Sakamoto

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

FtsH is an ATP-dependent metalloprotease present as a hexameric heterocomplex in thylakoid membranes. Encoded in the Arabidopsis thaliana YELLOW VARIEGATED2 (VAR2) locus, FtsH2 is one isoform among major Type A (FtsH1/5) and Type B (FtsH2/8) isomers. Mutants lacking FtsH2 (var2) and FtsH5 (var1) are characterized by a typical leaf-variegated phenotype. The functional importance of the catalytic center (comprised by the zinc binding domain) in FtsH2 was assessed in this study by generating transgenic plants that ectopically expressed FtsH2(488), a proteolytically inactive version of FtsH2. The resulting amino acid substitution inhibited FtsH protease activity in vivo when introduced into Escherichia coli FtsH. By contrast, expression of FtsH2(488) rescued not only leaf variegation in var2 but also seedling lethality in var2 ftsh8, suggesting that the protease activity of Type B isomers is completely dispensable, which implies that the chloroplastic FtsH complex has protease sites in excess and that they act redundantly rather than coordinately. However, expression of FtsH2 (488) did not fully rescue leaf variegation in var1 var2 because the overall FtsH levels were reduced under this background. Applying an inducible promoter to our complementation analysis revealed that rescue of leaf variegation indeed depends on the overall amount of FtsH. Our results elucidate protein activity and its amount as important factors for the function of FtsH heterocomplexes that are composed of multiple isoforms in the thylakoid membrane.

Original languageEnglish
Pages (from-to)3710-3725
Number of pages16
JournalPlant Cell
Volume22
Issue number11
DOIs
Publication statusPublished - Nov 2010

Fingerprint

Chloroplasts
Arabidopsis
Thylakoids
Peptide Hydrolases
proteinases
chloroplasts
Protein Isoforms
thylakoids
isomers
leaves
Genetically Modified Plants
Metalloproteases
Amino Acid Substitution
Seedlings
Zinc
metalloproteinases
amino acid substitution
Adenosine Triphosphate
Escherichia coli
Phenotype

ASJC Scopus subject areas

  • Plant Science
  • Cell Biology

Cite this

The FtsH protease heterocomplex in Arabidopsis : Dispensability of Type-B protease activity for proper chloroplast development. / Zhang, Di; Kato, Yusuke; Zhang, Lingang; Fujimoto, Masaru; Tsutsumi, Nobuhiro; Sodmergen, S.; Sakamoto, Wataru.

In: Plant Cell, Vol. 22, No. 11, 11.2010, p. 3710-3725.

Research output: Contribution to journalArticle

Zhang, Di ; Kato, Yusuke ; Zhang, Lingang ; Fujimoto, Masaru ; Tsutsumi, Nobuhiro ; Sodmergen, S. ; Sakamoto, Wataru. / The FtsH protease heterocomplex in Arabidopsis : Dispensability of Type-B protease activity for proper chloroplast development. In: Plant Cell. 2010 ; Vol. 22, No. 11. pp. 3710-3725.
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