The formation of g = 2.49-species of cytochrome P450 in the rat liver by PCB126 oral administration: Identification of heme axial ligands by EPR spectroscopy

Hidetoshi Morita, Hiroshi Yoshikawa, Tatsuya Takizawa, Mitsuyuki Shirai, Fumiaki Akahori, Tetsuhiko Yoshimura

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Rat livers and microsomes were subjected to electron paramagnetic resonance (EPR) measurements at 77 K. The EPR spectra of the livers from the control group, carbon tetrachloride-, 3-methylcholanthrene-, and 3,3′,4,4′, 5-pentachlorobiphenyl (PCB126)-treated rats exhibited an EPR spectrum at g = 2.40, 2.24, and 1.93, which is characteristic of P450 in a resting state. The liver of the PCB126-treated rats showed an additional distinct EPR spectrum at g = 2.49, 2.26, and 1.87 (g = 2.49-species). The heme environmental structure of g = 2.49-species was identified by crystal field analysis using three EPR g-values of the microsome treated with various chemicals. These results indicated that g = 2.49-species is a hemeprotein with cysteine thiolate at the 5th coordination site, and a nitrogenous ligand at the 6th site.

Original languageEnglish
Pages (from-to)2974-2981
Number of pages8
JournalBioscience, Biotechnology and Biochemistry
Volume70
Issue number12
DOIs
Publication statusPublished - 2006
Externally publishedYes

Fingerprint

electron paramagnetic resonance spectroscopy
Electron Spin Resonance Spectroscopy
heme
Heme
cytochrome P-450
Liver
Cytochrome P-450 Enzyme System
oral administration
Paramagnetic resonance
Oral Administration
Rats
Spectrum Analysis
Ligands
Spectroscopy
liver
rats
microsomes
Hemeproteins
carbon tetrachloride
Carbon tetrachloride

Keywords

  • 3,3′,4,4′, 5-pentachlorobiphenyl (PCB126)
  • Crystal field analysis
  • Low-spin Fe(III) hemeprotein

ASJC Scopus subject areas

  • Bioengineering
  • Biotechnology
  • Biochemistry
  • Biochemistry, Genetics and Molecular Biology(all)
  • Chemistry (miscellaneous)
  • Applied Microbiology and Biotechnology
  • Food Science

Cite this

The formation of g = 2.49-species of cytochrome P450 in the rat liver by PCB126 oral administration : Identification of heme axial ligands by EPR spectroscopy. / Morita, Hidetoshi; Yoshikawa, Hiroshi; Takizawa, Tatsuya; Shirai, Mitsuyuki; Akahori, Fumiaki; Yoshimura, Tetsuhiko.

In: Bioscience, Biotechnology and Biochemistry, Vol. 70, No. 12, 2006, p. 2974-2981.

Research output: Contribution to journalArticle

Morita, Hidetoshi ; Yoshikawa, Hiroshi ; Takizawa, Tatsuya ; Shirai, Mitsuyuki ; Akahori, Fumiaki ; Yoshimura, Tetsuhiko. / The formation of g = 2.49-species of cytochrome P450 in the rat liver by PCB126 oral administration : Identification of heme axial ligands by EPR spectroscopy. In: Bioscience, Biotechnology and Biochemistry. 2006 ; Vol. 70, No. 12. pp. 2974-2981.
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