TY - JOUR
T1 - The formation of capsule and septum in human hepatocellular carcinoma
AU - Ishizaki, Masahiko
AU - Ashida, Kozo
AU - Higashi, Toshihiro
AU - Nakatsukasa, Harushige
AU - Kaneyoshi, Toshihiko
AU - Fujiwara, Keishi
AU - Nouso, Kazuhiro
AU - Kobayashi, Yoshiyuki
AU - Uemura, Masayuki
AU - Nakamura, Shinichiro
AU - Tsuji, Takao
N1 - Funding Information:
Acknowledgements The authors thank Drs. Tatsuya Itoshima and Kazuo Hamaya, Internal Medicine and Pathology section, Okayama Saiseikai General Hospital, respectively, and the First Department of Surgery, Okayama University Medical School, for providing tissues. This work was supported by grants (no. 10670474) from the Intractable Liver Diseases Research Committee Education and (no. 12770259) from The Japanese Ministry of Health and Welfare.
Copyright:
Copyright 2007 Elsevier B.V., All rights reserved.
PY - 2001
Y1 - 2001
N2 - The formation of fibrous capsule around the cancer nodule and of the septum in the tumor is frequently observed with the development of hepatocellular carcinoma (HCC). We aimed to clarify how the capsule and septum were formed during the growth of HCC. Liver samples surgically resected from 25 patients with HCC were studied with in situ hybridization for type-I, -III, and -IV procollagen. Type-I and -III procollagen-expressing cells, mostly α-smooth muscle actin (SMA)-positive, were increased in the fibrous capsule and in the septum between HCC nodules. These cells were also found at the invasion front of HCC and around the necrotic cancer tissues. Type-IV procollagen gene expression was mainly observed in mesenchymal cells localized in both HCCs and non-cancerous liver. Cancer cells or hepatocytes did not express any of these procollagen genes. The present study reveals that the capsule and septum are mainly formed by α-SMA-positive mesen-chymal cells at the interface between two different tissues (e.g., cancer nodule vs non-cancerous liver or another cancer nodule). The wound healing occurs even in HCC. The capsule formation may result from interaction between tumor and host liver and interfere the growth and invasion of HCC.
AB - The formation of fibrous capsule around the cancer nodule and of the septum in the tumor is frequently observed with the development of hepatocellular carcinoma (HCC). We aimed to clarify how the capsule and septum were formed during the growth of HCC. Liver samples surgically resected from 25 patients with HCC were studied with in situ hybridization for type-I, -III, and -IV procollagen. Type-I and -III procollagen-expressing cells, mostly α-smooth muscle actin (SMA)-positive, were increased in the fibrous capsule and in the septum between HCC nodules. These cells were also found at the invasion front of HCC and around the necrotic cancer tissues. Type-IV procollagen gene expression was mainly observed in mesenchymal cells localized in both HCCs and non-cancerous liver. Cancer cells or hepatocytes did not express any of these procollagen genes. The present study reveals that the capsule and septum are mainly formed by α-SMA-positive mesen-chymal cells at the interface between two different tissues (e.g., cancer nodule vs non-cancerous liver or another cancer nodule). The wound healing occurs even in HCC. The capsule formation may result from interaction between tumor and host liver and interfere the growth and invasion of HCC.
KW - Collagens
KW - Hepatocellular carcinoma
KW - In situ hybridization
KW - Stellate cell
KW - α-Smooth muscle actin
UR - http://www.scopus.com/inward/record.url?scp=17844377852&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=17844377852&partnerID=8YFLogxK
U2 - 10.1007/s004280000391
DO - 10.1007/s004280000391
M3 - Article
C2 - 11469689
AN - SCOPUS:17844377852
VL - 438
SP - 574
EP - 580
JO - Virchows Archiv
JF - Virchows Archiv
SN - 0945-6317
IS - 6
ER -