The fluorescent probe method using cyanine dye to determine the membrane potential in cells

Tadaaki Kakutani, Ryuichi Nonaka, Yoshiyuki Murata, Ichiro Obi, Mitsugi Senda

Research output: Contribution to journalArticle

Abstract

Theoretical expressions were derived for the relationship between the fluorescence response of the cyanine dye, 3,3′-dipropylthiadicarbocyanine (diS-C3-(5)), added to a cell suspension and the membrane potential of the cell on the basis of the mechanism suggested by Sims et al. (Biochemistry, 13 (1974) 3315). The fluorescent probe method was applied to determine the membrane potential of protoplasts isolated from Lithospermum erythrorhizon cultured cells as a function of the concentrations of K+, Na+, Ca2+ and Mg2+ ions. The conditions which should be satisfied in evaluating the membrane potential by this method are discussed.

Original languageEnglish
Pages (from-to)221-233
Number of pages13
JournalJournal of Electroanalytical Chemistry
Volume343
Issue number1-2
DOIs
Publication statusPublished - 1992
Externally publishedYes

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Fluorescent Dyes
Coloring Agents
Dyes
Membranes
Biochemistry
Suspensions
Fluorescence
Cells
Ions

ASJC Scopus subject areas

  • Chemical Engineering(all)
  • Analytical Chemistry
  • Electrochemistry

Cite this

The fluorescent probe method using cyanine dye to determine the membrane potential in cells. / Kakutani, Tadaaki; Nonaka, Ryuichi; Murata, Yoshiyuki; Obi, Ichiro; Senda, Mitsugi.

In: Journal of Electroanalytical Chemistry, Vol. 343, No. 1-2, 1992, p. 221-233.

Research output: Contribution to journalArticle

Kakutani, Tadaaki ; Nonaka, Ryuichi ; Murata, Yoshiyuki ; Obi, Ichiro ; Senda, Mitsugi. / The fluorescent probe method using cyanine dye to determine the membrane potential in cells. In: Journal of Electroanalytical Chemistry. 1992 ; Vol. 343, No. 1-2. pp. 221-233.
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