The fluorescent probe method using cyanine dye to determine the membrane potential in cells

Tadaaki Kakutani; Ryuichi Nonaka; Yoshiyuki Murata; Ichiro Obi; Mitsugi Senda

doi:10.1016/0302-4598(92)80015-9

The fluorescent probe method using cyanine dye to determine the membrane potential in cells

Tadaaki Kakutani, Ryuichi Nonaka, Yoshiyuki Murata, Ichiro Obi, Mitsugi Senda

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

Theoretical expressions were derived for the relationship between the fluorescence response of the cyanine dye, 3,3′-dipropylthiadicarbocyanine (diS-C₃-(5)), added to a cell suspension and the membrane potential of the cell on the basis of the mechanism suggested by Sims et al. (Biochemistry, 13 (1974) 3315). The fluorescent probe method was applied to determine the membrane potential of protoplasts isolated from Lithospermum erythrorhizon cultured cells as a function of the concentrations of K⁺, Na⁺, Ca²⁺ and Mg²⁺ ions. The conditions which should be satisfied in evaluating the membrane potential by this method are discussed.

Original language	English
Pages (from-to)	221-233
Number of pages	13
Journal	Bioelectrochemistry and Bioenergetics
Volume	28
Issue number	1-2
DOIs	https://doi.org/10.1016/0302-4598(92)80015-9
Publication status	Published - Aug 1992
Externally published	Yes

ASJC Scopus subject areas

Biophysics
Physical and Theoretical Chemistry
Electrochemistry

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title = "The fluorescent probe method using cyanine dye to determine the membrane potential in cells",

abstract = "Theoretical expressions were derived for the relationship between the fluorescence response of the cyanine dye, 3,3′-dipropylthiadicarbocyanine (diS-C3-(5)), added to a cell suspension and the membrane potential of the cell on the basis of the mechanism suggested by Sims et al. (Biochemistry, 13 (1974) 3315). The fluorescent probe method was applied to determine the membrane potential of protoplasts isolated from Lithospermum erythrorhizon cultured cells as a function of the concentrations of K+, Na+, Ca2+ and Mg2+ ions. The conditions which should be satisfied in evaluating the membrane potential by this method are discussed.",

author = "Tadaaki Kakutani and Ryuichi Nonaka and Yoshiyuki Murata and Ichiro Obi and Mitsugi Senda",

year = "1992",

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T1 - The fluorescent probe method using cyanine dye to determine the membrane potential in cells

AU - Kakutani, Tadaaki

AU - Nonaka, Ryuichi

AU - Murata, Yoshiyuki

AU - Obi, Ichiro

AU - Senda, Mitsugi

PY - 1992/8

Y1 - 1992/8

N2 - Theoretical expressions were derived for the relationship between the fluorescence response of the cyanine dye, 3,3′-dipropylthiadicarbocyanine (diS-C3-(5)), added to a cell suspension and the membrane potential of the cell on the basis of the mechanism suggested by Sims et al. (Biochemistry, 13 (1974) 3315). The fluorescent probe method was applied to determine the membrane potential of protoplasts isolated from Lithospermum erythrorhizon cultured cells as a function of the concentrations of K+, Na+, Ca2+ and Mg2+ ions. The conditions which should be satisfied in evaluating the membrane potential by this method are discussed.

AB - Theoretical expressions were derived for the relationship between the fluorescence response of the cyanine dye, 3,3′-dipropylthiadicarbocyanine (diS-C3-(5)), added to a cell suspension and the membrane potential of the cell on the basis of the mechanism suggested by Sims et al. (Biochemistry, 13 (1974) 3315). The fluorescent probe method was applied to determine the membrane potential of protoplasts isolated from Lithospermum erythrorhizon cultured cells as a function of the concentrations of K+, Na+, Ca2+ and Mg2+ ions. The conditions which should be satisfied in evaluating the membrane potential by this method are discussed.

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