The fluorescent probe method using cyanine dye to determine the membrane potential in cells

Tadaaki Kakutani, Ryuichi Nonaka, Yoshiyuki Murata, Ichiro Obi, Mitsugi Senda

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Theoretical expressions were derived for the relationship between the fluorescence response of the cyanine dye, 3,3′-dipropylthiadicarbocyanine (diS-C3-(5)), added to a cell suspension and the membrane potential of the cell on the basis of the mechanism suggested by Sims et al. (Biochemistry, 13 (1974) 3315). The fluorescent probe method was applied to determine the membrane potential of protoplasts isolated from Lithospermum erythrorhizon cultured cells as a function of the concentrations of K+, Na+, Ca2+ and Mg2+ ions. The conditions which should be satisfied in evaluating the membrane potential by this method are discussed.

Original languageEnglish
Pages (from-to)221-233
Number of pages13
JournalBioelectrochemistry and Bioenergetics
Volume28
Issue number1-2
DOIs
Publication statusPublished - 1992
Externally publishedYes

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Fluorescent Dyes
Membrane Potentials
Coloring Agents
Dyes
dyes
membranes
Membranes
Lithospermum
protoplasts
cells
biochemistry
Biochemistry
Protoplasts
cultured cells
Cultured Cells
Suspensions
Fluorescence
Cells
Cell Membrane
Ions

ASJC Scopus subject areas

  • Biochemistry

Cite this

The fluorescent probe method using cyanine dye to determine the membrane potential in cells. / Kakutani, Tadaaki; Nonaka, Ryuichi; Murata, Yoshiyuki; Obi, Ichiro; Senda, Mitsugi.

In: Bioelectrochemistry and Bioenergetics, Vol. 28, No. 1-2, 1992, p. 221-233.

Research output: Contribution to journalArticle

Kakutani, Tadaaki ; Nonaka, Ryuichi ; Murata, Yoshiyuki ; Obi, Ichiro ; Senda, Mitsugi. / The fluorescent probe method using cyanine dye to determine the membrane potential in cells. In: Bioelectrochemistry and Bioenergetics. 1992 ; Vol. 28, No. 1-2. pp. 221-233.
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