Abstract
Theoretical expressions were derived for the relationship between the fluorescence response of the cyanine dye, 3,3′-dipropylthiadicarbocyanine (diS-C3-(5)), added to a cell suspension and the membrane potential of the cell on the basis of the mechanism suggested by Sims et al. (Biochemistry, 13 (1974) 3315). The fluorescent probe method was applied to determine the membrane potential of protoplasts isolated from Lithospermum erythrorhizon cultured cells as a function of the concentrations of K+, Na+, Ca2+ and Mg2+ ions. The conditions which should be satisfied in evaluating the membrane potential by this method are discussed.
| Original language | English |
|---|---|
| Pages (from-to) | 221-233 |
| Number of pages | 13 |
| Journal | Bioelectrochemistry and Bioenergetics |
| Volume | 28 |
| Issue number | 1-2 |
| DOIs | |
| Publication status | Published - 1992 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Biochemistry
Cite this
The fluorescent probe method using cyanine dye to determine the membrane potential in cells. / Kakutani, Tadaaki; Nonaka, Ryuichi; Murata, Yoshiyuki; Obi, Ichiro; Senda, Mitsugi.
In: Bioelectrochemistry and Bioenergetics, Vol. 28, No. 1-2, 1992, p. 221-233.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The fluorescent probe method using cyanine dye to determine the membrane potential in cells
AU - Kakutani, Tadaaki
AU - Nonaka, Ryuichi
AU - Murata, Yoshiyuki
AU - Obi, Ichiro
AU - Senda, Mitsugi
PY - 1992
Y1 - 1992
N2 - Theoretical expressions were derived for the relationship between the fluorescence response of the cyanine dye, 3,3′-dipropylthiadicarbocyanine (diS-C3-(5)), added to a cell suspension and the membrane potential of the cell on the basis of the mechanism suggested by Sims et al. (Biochemistry, 13 (1974) 3315). The fluorescent probe method was applied to determine the membrane potential of protoplasts isolated from Lithospermum erythrorhizon cultured cells as a function of the concentrations of K+, Na+, Ca2+ and Mg2+ ions. The conditions which should be satisfied in evaluating the membrane potential by this method are discussed.
AB - Theoretical expressions were derived for the relationship between the fluorescence response of the cyanine dye, 3,3′-dipropylthiadicarbocyanine (diS-C3-(5)), added to a cell suspension and the membrane potential of the cell on the basis of the mechanism suggested by Sims et al. (Biochemistry, 13 (1974) 3315). The fluorescent probe method was applied to determine the membrane potential of protoplasts isolated from Lithospermum erythrorhizon cultured cells as a function of the concentrations of K+, Na+, Ca2+ and Mg2+ ions. The conditions which should be satisfied in evaluating the membrane potential by this method are discussed.
UR - http://www.scopus.com/inward/record.url?scp=3643132454&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=3643132454&partnerID=8YFLogxK
U2 - 10.1016/0302-4598(92)80015-9
DO - 10.1016/0302-4598(92)80015-9
M3 - Article
VL - 28
SP - 221
EP - 233
JO - Bioelectrochemistry
JF - Bioelectrochemistry
SN - 1567-5394
IS - 1-2
ER -