The first step in polyethylene glycol degradation by sphingomonads proceeds via a flavoprotein alcohol dehydrogenase containing flavin adenine dinucleotide

Manabu Sugimoto, M. Tanabe, M. Hataya, S. Enokibara, J. A. Duine, F. Kawai

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Several Sphingomonas spp. utilize polyethylene glycols (PEGs) as a sole carbon and energy source, oxidative PEG degradation being initiated by a dye-linked dehydrogenase (PEG-DH) that oxidizes the terminal alcohol groups of the polymer chain. Purification and characterization of PEG-DH from Sphingomonas terrae revealed that the enzyme is membrane bound. The gene encoding this enzyme (pegA) was cloned, sequenced, and expressed in Escherichia coli. The purified recombinant enzyme was vulnerable to aggregation and inactivation, but this could be prevented by addition of detergent. It is as a homodimeric protein with a subunit molecular mass of 58.8 kDa, each subunit containing 1 noncovalently bound flavin adenine dinucleotide but not Fe or Zn. PEG-DH recognizes a broad variety of primary aliphatic and aromatic alcohols as substrates. Comparison with known sequences revealed that PEG-DH belongs to the group of glucose-methanol-choline (GMC) flavoprotein oxidoreductases and that it is a novel type of flavoprotein alcohol dehydrogenase related (percent identical amino acids) to other, so far uncharacterized bacterial, membrane-bound, dye-linked dehydrogenases: alcohol dehydrogenase from Pseudomonas oleovorans (46%); choline dehydrogenase from E. coli (40%); L-sorbose dehydrogenase from Gluconobacter oxydans (38%); and 4-nitrobenzyl alcohol dehydrogenase from a Pseudomonas species (35%).

Original languageEnglish
Pages (from-to)6694-6698
Number of pages5
JournalJournal of Bacteriology
Volume183
Issue number22
DOIs
Publication statusPublished - 2001

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Flavoproteins
Flavin-Adenine Dinucleotide
Alcohol Dehydrogenase
Sphingomonas
Choline Dehydrogenase
Pseudomonas oleovorans
Oxidoreductases
Gluconobacter oxydans
Coloring Agents
Enzymes
Alcohols
Escherichia coli
Membranes
Choline
Pseudomonas
Detergents
Methanol
Polymers
Carbon
Amino Acids

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Immunology

Cite this

The first step in polyethylene glycol degradation by sphingomonads proceeds via a flavoprotein alcohol dehydrogenase containing flavin adenine dinucleotide. / Sugimoto, Manabu; Tanabe, M.; Hataya, M.; Enokibara, S.; Duine, J. A.; Kawai, F.

In: Journal of Bacteriology, Vol. 183, No. 22, 2001, p. 6694-6698.

Research output: Contribution to journalArticle

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