The expression of the nuclear receptors NR5A1 and NR5A2 and transcription factor GATA6 correlates with steroidogenic gene expression in the bovine corpus luteum

Hiroaki Taniguchi, Junichi Komiyama, Robert S. Viger, Kiyoshi Okuda

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19 Citations (Scopus)

Abstract

The corpus luteum (CL) is the major site of progesterone (P4) production during the luteal phase of the estrous cycle in cattle. To better understand the molecular mechanisms underlying P4 production, we compared the mRNA and protein expression profiles of key components of the steroidogenic pathway (StAR, CYP11A, and 3β-HSD) during the bovine CL luteal phase with that of several transcription factors (NR5A1, NR5A2, GATA4, GATA6) known for their roles in the control of steroidogenic gene expression. In the bovine CL, StAR, CYP11A, and 3β-HSD mRNA and protein levels remained constant at the mid and late luteal phases but markedly declined at the regressed luteal stage. NR5A1 and NR5A2 exhibited a similar pattern with a significant decrease in expression at the regressed luteal stage. Both GATA4 and GATA6 mRNA and proteins could be detected in bovine CL; GATA6 levels, however, were generally higher. Although GATA4 expression did not change during the luteal phase, GATA6 showed a marked decrease at the regressed luteal stage, like NR5A1, NR5A2, and the other steroidogenic markers. Thus, we suggest that NR5A1, NR5A2, and GATA6, but not GATA4, contribute to the transcriptional regulation of steroidogenic gene expression, and hence P4 production, in the bovine CL. Furthermore, we have demonstrated the association of NR5A1 and NR5A2 with the bovine StAR promoter in the mid-luteal CL using chromatin immunoprecipitation, suggesting that these factors have definitive roles in the regulation of StAR gene transcription in vivo.

Original languageEnglish
Pages (from-to)873-880
Number of pages8
JournalMolecular Reproduction and Development
Volume76
Issue number9
DOIs
Publication statusPublished - Sep 2009

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GATA6 Transcription Factor
Corpus Luteum
Cytoplasmic and Nuclear Receptors
Gene Expression
Luteal Phase
Messenger RNA
Proteins
Estrous Cycle
Chromatin Immunoprecipitation
Gene Expression Regulation

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology
  • Cell Biology

Cite this

The expression of the nuclear receptors NR5A1 and NR5A2 and transcription factor GATA6 correlates with steroidogenic gene expression in the bovine corpus luteum. / Taniguchi, Hiroaki; Komiyama, Junichi; Viger, Robert S.; Okuda, Kiyoshi.

In: Molecular Reproduction and Development, Vol. 76, No. 9, 09.2009, p. 873-880.

Research output: Contribution to journalArticle

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