The expression of podoplanin and classic cadherins in the mouse brain

Chiaki Kaji, Miwa Tomooka, Yukinari Kato, Hiroshi Kojima, Yoshihiko Sawa

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Podoplanin is a transmembrane glycoprotein indirectly linked to classic cadherins through ezrin-actin networks. Recently, the overexpression of podoplanin in high-grade malignancy brain tumors has been reported. The aim of this study was to investigate the expression of podoplanin and classic cadherins in the mouse brain. Immunohistochemistry showed that podoplanin was expressed on ependymal cells and choroid plexus epithelial cells at the ventricle side of the cell surface and at the cell-cell junctions, and on retinal pigment epithelial cells and in the pia mater; P-cadherin between choroid plexus epithelial cells and endothelial cells at the basement membrane side of cell surface, and between retinal pigment epithelial cells; VE-cadherin on the PECAM-1 positive-choroid plexus endothelial cells of the fibrovascular core; and N-cadherin on the cell surface and at the cell-cell junctions of ependymal cells, and in the pia mater. The regions expressing podoplanin, P-cadherin, and VE-cadherin did not coincide. In real-time PCR analysis, the amounts of podoplanin and P- and N-cadherin mRNA were larger in the ventricular wall with choroid plexus than in the abdominal aorta and cerebrum. In the RT-PCR analysis, the intensities of amplicon for VE-cadherin mRNA were the same for the abdominal aorta, cerebrum, and ventricular wall with the choroid plexus, suggesting that mouse ependymal cells, choroid plexus epithelial cells, and glial cells under the pia mater have the ability to express podoplanin and P- and N-cadherins. Glial cells and retinal pigment epithelial cells may create barriers by podoplanin and classic cadherins as a rate-determining step for transmission of blood components.

Original languageEnglish
Pages (from-to)435-446
Number of pages12
JournalJournal of Anatomy
Volume220
Issue number5
DOIs
Publication statusPublished - May 2012
Externally publishedYes

Fingerprint

cadherins
Cadherins
Choroid Plexus
pigment
brain
choroid plexus
Pia Mater
Epithelial Cells
mice
Brain
Retinal Pigments
epithelial cells
tumor
Intercellular Junctions
Abdominal Aorta
Cerebrum
cells
blood
intercellular junctions
Neuroglia

Keywords

  • Choroid plexus
  • Functional human anatomy
  • P-cadherin
  • Podoplanin

ASJC Scopus subject areas

  • Anatomy
  • Histology
  • Developmental Biology
  • Ecology, Evolution, Behavior and Systematics
  • Molecular Biology
  • Cell Biology

Cite this

The expression of podoplanin and classic cadherins in the mouse brain. / Kaji, Chiaki; Tomooka, Miwa; Kato, Yukinari; Kojima, Hiroshi; Sawa, Yoshihiko.

In: Journal of Anatomy, Vol. 220, No. 5, 05.2012, p. 435-446.

Research output: Contribution to journalArticle

Kaji, Chiaki ; Tomooka, Miwa ; Kato, Yukinari ; Kojima, Hiroshi ; Sawa, Yoshihiko. / The expression of podoplanin and classic cadherins in the mouse brain. In: Journal of Anatomy. 2012 ; Vol. 220, No. 5. pp. 435-446.
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