The expression of HLA on two cultured choriocarcinoma cell lines

M. Tanimoto, T. Takahashi, Y. Nishizuka

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Since gestational choriocarcinoma is believed to be derived from trophoblast, paternal antigens are expected to be present on carcinoma cells. Two choriocarcinoma lines (GCC-SV and Lu-75) transplantable in nude mice were obtained and cultures were started in order to study the expression of HLA on these tumor cells. To date, two human chorionic gonadotropin-producing cell lines, designated GCC-SV(c) and Lu-75(c), have been maintained for over 2 years with more than 15 passages and have been employed for serological studies. First, GCC-SV(c) (patient A2, AW30, B8, B10; husband A3, AW26, BW16, BW35) was tested by protein A assay with HLA-A typing sera and anti-β2 microglobulin; the phenotype was A2+, A3-, AW26-, β2+, which corresponded to HLA-A type of the patient. In contrast HLA-A was not demonstrated on Lu-75(c) cells (patient A2, BW35, BW39; husband A9, AW26, BW22) by the direct test, but anti-β2 microglobulin serum gave a positive reaction. A mouse monoclonal antibody against HLA was also employed, but it did not react against Lu-75(c) cells. Further serological analysis was carried out by means of absorption tests, and it was confirmed that GCC-SV(c) was A2+, A3-, AW26- and Lu-75(c) was A2-, A9-, AW26-. The results so far obtained do not demonstrate the expression of paternal HLA-A antigens on choriocarcinoma cells.

Original languageEnglish
Pages (from-to)27-34
Number of pages8
JournalGann, The Japanese Journal of Cancer Research
Volume73
Issue number1
Publication statusPublished - 1982
Externally publishedYes

Fingerprint

Choriocarcinoma
varespladib methyl
HLA-A Antigens
Cultured Cells
Cell Line
Spouses
Histocompatibility Testing
Staphylococcal Protein A
Trophoblasts
Chorionic Gonadotropin
Serum
Nude Mice
Monoclonal Antibodies
Carcinoma
Phenotype
Antigens
Neoplasms

ASJC Scopus subject areas

  • Cancer Research

Cite this

The expression of HLA on two cultured choriocarcinoma cell lines. / Tanimoto, M.; Takahashi, T.; Nishizuka, Y.

In: Gann, The Japanese Journal of Cancer Research, Vol. 73, No. 1, 1982, p. 27-34.

Research output: Contribution to journalArticle

Tanimoto, M, Takahashi, T & Nishizuka, Y 1982, 'The expression of HLA on two cultured choriocarcinoma cell lines', Gann, The Japanese Journal of Cancer Research, vol. 73, no. 1, pp. 27-34.
Tanimoto, M. ; Takahashi, T. ; Nishizuka, Y. / The expression of HLA on two cultured choriocarcinoma cell lines. In: Gann, The Japanese Journal of Cancer Research. 1982 ; Vol. 73, No. 1. pp. 27-34.
@article{824a8bff924f4b8bb6ad22593b5f24fb,
title = "The expression of HLA on two cultured choriocarcinoma cell lines",
abstract = "Since gestational choriocarcinoma is believed to be derived from trophoblast, paternal antigens are expected to be present on carcinoma cells. Two choriocarcinoma lines (GCC-SV and Lu-75) transplantable in nude mice were obtained and cultures were started in order to study the expression of HLA on these tumor cells. To date, two human chorionic gonadotropin-producing cell lines, designated GCC-SV(c) and Lu-75(c), have been maintained for over 2 years with more than 15 passages and have been employed for serological studies. First, GCC-SV(c) (patient A2, AW30, B8, B10; husband A3, AW26, BW16, BW35) was tested by protein A assay with HLA-A typing sera and anti-β2 microglobulin; the phenotype was A2+, A3-, AW26-, β2+, which corresponded to HLA-A type of the patient. In contrast HLA-A was not demonstrated on Lu-75(c) cells (patient A2, BW35, BW39; husband A9, AW26, BW22) by the direct test, but anti-β2 microglobulin serum gave a positive reaction. A mouse monoclonal antibody against HLA was also employed, but it did not react against Lu-75(c) cells. Further serological analysis was carried out by means of absorption tests, and it was confirmed that GCC-SV(c) was A2+, A3-, AW26- and Lu-75(c) was A2-, A9-, AW26-. The results so far obtained do not demonstrate the expression of paternal HLA-A antigens on choriocarcinoma cells.",
author = "M. Tanimoto and T. Takahashi and Y. Nishizuka",
year = "1982",
language = "English",
volume = "73",
pages = "27--34",
journal = "Cancer Science",
issn = "1347-9032",
publisher = "Wiley-Blackwell",
number = "1",

}

TY - JOUR

T1 - The expression of HLA on two cultured choriocarcinoma cell lines

AU - Tanimoto, M.

AU - Takahashi, T.

AU - Nishizuka, Y.

PY - 1982

Y1 - 1982

N2 - Since gestational choriocarcinoma is believed to be derived from trophoblast, paternal antigens are expected to be present on carcinoma cells. Two choriocarcinoma lines (GCC-SV and Lu-75) transplantable in nude mice were obtained and cultures were started in order to study the expression of HLA on these tumor cells. To date, two human chorionic gonadotropin-producing cell lines, designated GCC-SV(c) and Lu-75(c), have been maintained for over 2 years with more than 15 passages and have been employed for serological studies. First, GCC-SV(c) (patient A2, AW30, B8, B10; husband A3, AW26, BW16, BW35) was tested by protein A assay with HLA-A typing sera and anti-β2 microglobulin; the phenotype was A2+, A3-, AW26-, β2+, which corresponded to HLA-A type of the patient. In contrast HLA-A was not demonstrated on Lu-75(c) cells (patient A2, BW35, BW39; husband A9, AW26, BW22) by the direct test, but anti-β2 microglobulin serum gave a positive reaction. A mouse monoclonal antibody against HLA was also employed, but it did not react against Lu-75(c) cells. Further serological analysis was carried out by means of absorption tests, and it was confirmed that GCC-SV(c) was A2+, A3-, AW26- and Lu-75(c) was A2-, A9-, AW26-. The results so far obtained do not demonstrate the expression of paternal HLA-A antigens on choriocarcinoma cells.

AB - Since gestational choriocarcinoma is believed to be derived from trophoblast, paternal antigens are expected to be present on carcinoma cells. Two choriocarcinoma lines (GCC-SV and Lu-75) transplantable in nude mice were obtained and cultures were started in order to study the expression of HLA on these tumor cells. To date, two human chorionic gonadotropin-producing cell lines, designated GCC-SV(c) and Lu-75(c), have been maintained for over 2 years with more than 15 passages and have been employed for serological studies. First, GCC-SV(c) (patient A2, AW30, B8, B10; husband A3, AW26, BW16, BW35) was tested by protein A assay with HLA-A typing sera and anti-β2 microglobulin; the phenotype was A2+, A3-, AW26-, β2+, which corresponded to HLA-A type of the patient. In contrast HLA-A was not demonstrated on Lu-75(c) cells (patient A2, BW35, BW39; husband A9, AW26, BW22) by the direct test, but anti-β2 microglobulin serum gave a positive reaction. A mouse monoclonal antibody against HLA was also employed, but it did not react against Lu-75(c) cells. Further serological analysis was carried out by means of absorption tests, and it was confirmed that GCC-SV(c) was A2+, A3-, AW26- and Lu-75(c) was A2-, A9-, AW26-. The results so far obtained do not demonstrate the expression of paternal HLA-A antigens on choriocarcinoma cells.

UR - http://www.scopus.com/inward/record.url?scp=0020085072&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0020085072&partnerID=8YFLogxK

M3 - Article

C2 - 6956527

AN - SCOPUS:0020085072

VL - 73

SP - 27

EP - 34

JO - Cancer Science

JF - Cancer Science

SN - 1347-9032

IS - 1

ER -