The expression of E-selectin and chemokines in the cultured human lymphatic endothelium with lipopolysaccharides

Yoshihiko Sawa, Eichi Tsuruga

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

This study investigated the expression of selectins and chemokines in cultured human lymphatic endothelial cells stimulated with lipopolysaccharides. In microarray, vascular cell adhesion molecule-1 and intercellular adhesion molecule-1 gene expressions in the lymphatic endothelium with lipopolysaccharides did not change at 0.5 h but increased two- to three-fold at 12 h, whereas E-selectin increased 10-fold at 0.5 h and 68-fold at 12 h compared with untreated cells. The E-selectin mRNA and protein increased in the lymphatic endothelial cells with lipopolysaccharides at more than two-fold levels compared with human umbilical vein endothelial cells. Induction of Cys-Cys chemokine ligand 2, 3, 5, 7, 8 and 20 mRNAs in the lymphatic endothelial cells with lipopolysaccharides was detected in microarray and real-time PCR. The Cys-Cys chemokine ligand 2, 5 and 20 mRNA amounts in cells with high concentration lipopolysaccharides were larger in the lymphatic endothelial cells than in human umbilical vein endothelial cells. The Cys-Cys chemokine ligand 3 and 8 mRNAs were not detected in human umbilical vein endothelial cells. Induction of Cys-X-Cys chemokine ligand 1, 3, 5, 6 and 8 mRNAs was detected in the lymphatic endothelial cells with lipopolysaccharides. The Cys-X-Cys chemokine ligand 3, 5 and 8 mRNA amounts in cells with high concentration lipopolysaccharides were larger in the lymphatic endothelial cells than in human umbilical vein endothelial cells. In conclusion, it was demonstrated that the cultured human lymphatic endothelial cells express E-selectin and phagocyte-attractive chemokine genes.

Original languageEnglish
Pages (from-to)654-663
Number of pages10
JournalJournal of Anatomy
Volume212
Issue number5
DOIs
Publication statusPublished - May 2008
Externally publishedYes

Fingerprint

Lymphatic Endothelium
E-Selectin
chemokines
endothelium
Chemokines
lipopolysaccharides
endothelial cells
ligand
Lipopolysaccharides
Endothelial Cells
cysteinylcysteine
Human Umbilical Vein Endothelial Cells
fold
Messenger RNA
Ligands
adhesion
Selectins
gene expression
Vascular Cell Adhesion Molecule-1
phagocytes

Keywords

  • CCL
  • CXCL
  • E-selectin
  • Lymphatic endothelium

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Anatomy

Cite this

The expression of E-selectin and chemokines in the cultured human lymphatic endothelium with lipopolysaccharides. / Sawa, Yoshihiko; Tsuruga, Eichi.

In: Journal of Anatomy, Vol. 212, No. 5, 05.2008, p. 654-663.

Research output: Contribution to journalArticle

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