The expanding superfamily of gelsolin homology domain proteins

Umesh Ghoshdastider, David Popp, Leslie D. Burtnick, Robert C. Robinson

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

The gelsolin homology (GH) domain has been found to date exclusively in actin-binding proteins. In humans, three copies of the domain are present in CapG, five copies in supervillin, and six copies each in adseverin, gelsolin, flightless I and the villins: villin, advillin and villin-like protein. Caenorhabditis elegans contains a four-GH-domain protein, GSNL-1. These architectures are predicted to have arisen from gene triplication followed by gene duplication to result in the six-domain protein. The subsequent loss of one, two or three domains produced the five-, four-, and three-domain proteins, respectively. Here we conducted BLAST and hidden Markov based searches of UniProt and NCBI databases to identify novel gelsolin domain containing proteins. The variety in architectures suggests that the GH domain has been tested in many molecular constructions during evolution. Of particular note is flightless-like I protein (FLIIL1) from Entamoeba histolytica, which combines a leucine rich repeats (LRR) domain, seven GH domains, and a headpiece domain, thus combining many of the features of flightless I with those of villin or supervillin. As such, the GH domain superfamily appears to have developed along complex routes. The distribution of these proteins was analyzed in the 343 completely sequenced genomes, mapped onto the tree of life, and phylogenetic trees of the proteins were constructed to gain insight into their evolution.

Original languageEnglish
Pages (from-to)775-795
Number of pages21
JournalCytoskeleton
Volume70
Issue number11
DOIs
Publication statusPublished - Nov 1 2013
Externally publishedYes

Fingerprint

Gelsolin
Proteins
Microfilament Proteins
Entamoeba histolytica
Gene Duplication
Caenorhabditis elegans
Protein Domains
Leucine
Genome
Databases
villin

Keywords

  • Actin
  • Flightless
  • Gelsolin
  • Supervillin
  • Villin

ASJC Scopus subject areas

  • Structural Biology
  • Cell Biology

Cite this

The expanding superfamily of gelsolin homology domain proteins. / Ghoshdastider, Umesh; Popp, David; Burtnick, Leslie D.; Robinson, Robert C.

In: Cytoskeleton, Vol. 70, No. 11, 01.11.2013, p. 775-795.

Research output: Contribution to journalArticle

Ghoshdastider, U, Popp, D, Burtnick, LD & Robinson, RC 2013, 'The expanding superfamily of gelsolin homology domain proteins', Cytoskeleton, vol. 70, no. 11, pp. 775-795. https://doi.org/10.1002/cm.21149
Ghoshdastider, Umesh ; Popp, David ; Burtnick, Leslie D. ; Robinson, Robert C. / The expanding superfamily of gelsolin homology domain proteins. In: Cytoskeleton. 2013 ; Vol. 70, No. 11. pp. 775-795.
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