The exp1 gene essential for pileus expansion and autolysis of the inky cap mushroom Coprinopsis cinerea (Coprinus cinereus) encodes an HMG protein

Hajime Muraguchi, Takashi Fujita, Yuya Kishibe, Kanako Konno, Nanae Ueda, Kiyoshi Nakahori, Sonoe O. Yanagi, Takashi Kamada

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

The homobasidiomycete Coprinopsis cinerea is a member of the fungi known as inky cap mushrooms, and its fruiting-body pileus autolyzes soon after completion of the development. During the last 3 h of the development, the pileus exhibits umbrella-like expansion: the pileal tissue is cracked at the base of each gill and then each gill tissue is split to form a V-shape, as seen in a cross section. We identified two C. cinerea mutants defective in both pileus expansion and autolysis. The defects in both mutants are due to recessive mutations in a single gene, designated exp1. The exp1 gene is predicted to encode an HMG1/2-like protein with two HMG domains. The transcription of exp1 is strongly induced in the pileus 3 h before pileus expansion. This result, together with the fact that the exp1 mutations cause a specific developmental phenotype, suggest that Exp1 is a novel, transcriptional regulator controlling the final phase of fruiting-body morphogenesis.

Original languageEnglish
Pages (from-to)890-896
Number of pages7
JournalFungal Genetics and Biology
Volume45
Issue number6
DOIs
Publication statusPublished - Jun 2008

Fingerprint

Coprinus
High Mobility Group Proteins
Autolysis
Agaricales
Essential Genes
Tissue Expansion
HMGB1 Protein
Mutation
Morphogenesis
Genes
Fungi
Phenotype
Proteins

Keywords

  • Autolysis
  • Cell-to-cell adhesion
  • Coprinopsis cinerea
  • Coprinus cinereus
  • Fruiting-body morphogenesis
  • HMG1/2
  • Homobasidiomycete
  • Pileus expansion
  • Transcriptional regulator

ASJC Scopus subject areas

  • Genetics
  • Microbiology

Cite this

The exp1 gene essential for pileus expansion and autolysis of the inky cap mushroom Coprinopsis cinerea (Coprinus cinereus) encodes an HMG protein. / Muraguchi, Hajime; Fujita, Takashi; Kishibe, Yuya; Konno, Kanako; Ueda, Nanae; Nakahori, Kiyoshi; Yanagi, Sonoe O.; Kamada, Takashi.

In: Fungal Genetics and Biology, Vol. 45, No. 6, 06.2008, p. 890-896.

Research output: Contribution to journalArticle

Muraguchi, Hajime ; Fujita, Takashi ; Kishibe, Yuya ; Konno, Kanako ; Ueda, Nanae ; Nakahori, Kiyoshi ; Yanagi, Sonoe O. ; Kamada, Takashi. / The exp1 gene essential for pileus expansion and autolysis of the inky cap mushroom Coprinopsis cinerea (Coprinus cinereus) encodes an HMG protein. In: Fungal Genetics and Biology. 2008 ; Vol. 45, No. 6. pp. 890-896.
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