Abstract
The homobasidiomycete Coprinopsis cinerea is a member of the fungi known as inky cap mushrooms, and its fruiting-body pileus autolyzes soon after completion of the development. During the last 3 h of the development, the pileus exhibits umbrella-like expansion: the pileal tissue is cracked at the base of each gill and then each gill tissue is split to form a V-shape, as seen in a cross section. We identified two C. cinerea mutants defective in both pileus expansion and autolysis. The defects in both mutants are due to recessive mutations in a single gene, designated exp1. The exp1 gene is predicted to encode an HMG1/2-like protein with two HMG domains. The transcription of exp1 is strongly induced in the pileus 3 h before pileus expansion. This result, together with the fact that the exp1 mutations cause a specific developmental phenotype, suggest that Exp1 is a novel, transcriptional regulator controlling the final phase of fruiting-body morphogenesis.
| Original language | English |
|---|---|
| Pages (from-to) | 890-896 |
| Number of pages | 7 |
| Journal | Fungal Genetics and Biology |
| Volume | 45 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - Jun 2008 |
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Keywords
- Autolysis
- Cell-to-cell adhesion
- Coprinopsis cinerea
- Coprinus cinereus
- Fruiting-body morphogenesis
- HMG1/2
- Homobasidiomycete
- Pileus expansion
- Transcriptional regulator
ASJC Scopus subject areas
- Genetics
- Microbiology
Cite this
The exp1 gene essential for pileus expansion and autolysis of the inky cap mushroom Coprinopsis cinerea (Coprinus cinereus) encodes an HMG protein. / Muraguchi, Hajime; Fujita, Takashi; Kishibe, Yuya; Konno, Kanako; Ueda, Nanae; Nakahori, Kiyoshi; Yanagi, Sonoe O.; Kamada, Takashi.
In: Fungal Genetics and Biology, Vol. 45, No. 6, 06.2008, p. 890-896.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The exp1 gene essential for pileus expansion and autolysis of the inky cap mushroom Coprinopsis cinerea (Coprinus cinereus) encodes an HMG protein
AU - Muraguchi, Hajime
AU - Fujita, Takashi
AU - Kishibe, Yuya
AU - Konno, Kanako
AU - Ueda, Nanae
AU - Nakahori, Kiyoshi
AU - Yanagi, Sonoe O.
AU - Kamada, Takashi
PY - 2008/6
Y1 - 2008/6
N2 - The homobasidiomycete Coprinopsis cinerea is a member of the fungi known as inky cap mushrooms, and its fruiting-body pileus autolyzes soon after completion of the development. During the last 3 h of the development, the pileus exhibits umbrella-like expansion: the pileal tissue is cracked at the base of each gill and then each gill tissue is split to form a V-shape, as seen in a cross section. We identified two C. cinerea mutants defective in both pileus expansion and autolysis. The defects in both mutants are due to recessive mutations in a single gene, designated exp1. The exp1 gene is predicted to encode an HMG1/2-like protein with two HMG domains. The transcription of exp1 is strongly induced in the pileus 3 h before pileus expansion. This result, together with the fact that the exp1 mutations cause a specific developmental phenotype, suggest that Exp1 is a novel, transcriptional regulator controlling the final phase of fruiting-body morphogenesis.
AB - The homobasidiomycete Coprinopsis cinerea is a member of the fungi known as inky cap mushrooms, and its fruiting-body pileus autolyzes soon after completion of the development. During the last 3 h of the development, the pileus exhibits umbrella-like expansion: the pileal tissue is cracked at the base of each gill and then each gill tissue is split to form a V-shape, as seen in a cross section. We identified two C. cinerea mutants defective in both pileus expansion and autolysis. The defects in both mutants are due to recessive mutations in a single gene, designated exp1. The exp1 gene is predicted to encode an HMG1/2-like protein with two HMG domains. The transcription of exp1 is strongly induced in the pileus 3 h before pileus expansion. This result, together with the fact that the exp1 mutations cause a specific developmental phenotype, suggest that Exp1 is a novel, transcriptional regulator controlling the final phase of fruiting-body morphogenesis.
KW - Autolysis
KW - Cell-to-cell adhesion
KW - Coprinopsis cinerea
KW - Coprinus cinereus
KW - Fruiting-body morphogenesis
KW - HMG1/2
KW - Homobasidiomycete
KW - Pileus expansion
KW - Transcriptional regulator
UR - http://www.scopus.com/inward/record.url?scp=43049165457&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=43049165457&partnerID=8YFLogxK
U2 - 10.1016/j.fgb.2007.11.004
DO - 10.1016/j.fgb.2007.11.004
M3 - Article
C2 - 18164224
AN - SCOPUS:43049165457
VL - 45
SP - 890
EP - 896
JO - Fungal Genetics and Biology
JF - Fungal Genetics and Biology
SN - 1087-1845
IS - 6
ER -