TY - JOUR
T1 - The ex vivo effects of eyestalk peptides on ovarian vitellogenin gene expression in the kuruma prawn Marsupenaeus japonicus
AU - Tsutsui, Naoaki
AU - Nagakura-Nakamura, Ayano
AU - Nagai, Chiaki
AU - Ohira, Tsuyoshi
AU - Wilder, Marcy N.
AU - Nagasawa, Hiromichi
N1 - Funding Information:
Acknowledgments We are grateful to Dr. Takeshi Kawai of the Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, for preparing Pej-PDH-II. This work was partly supported by a Fisheries Research Agency project and a Grant-in-Aid for Scientific Research (no. 21780186) from the Ministry of Education, Culture, Sport, Science and Technology of Japan. N.T. and C.N. were supported by a Research Fellowship of the Japan Society for the Promotion of Science for Young Scientists.
PY - 2013/1
Y1 - 2013/1
N2 - Seven major peptides belonging to the crustacean hyperglycemic hormone family were purified from the sinus gland located in the eyestalk of the kuruma prawn Marsupenaeus japonicus, and their effects on vitellogenin gene expression were examined using the ex vivo ovary incubation system. Six molecular species of crustacean hyperglycemic hormone, Pej-SGP-I, -II, -III, -V, VI, and VII, displayed significant inhibitory effects on vg expression with almost the same efficacies, whereas Pej-SGP-IV (known as molt-inhibiting hormone) did not. Two chromatophorotropic peptides, red pigment-concentrating hormone and pigment-dispersing hormone, which were also present in the sinus glands, did not have a clear effect on the gene expression levels in this incubation system. These results suggest that the six crustacean hyperglycemic hormones are potentially capable of acting as vitellogenesis-inhibiting hormones in M. japonicus.
AB - Seven major peptides belonging to the crustacean hyperglycemic hormone family were purified from the sinus gland located in the eyestalk of the kuruma prawn Marsupenaeus japonicus, and their effects on vitellogenin gene expression were examined using the ex vivo ovary incubation system. Six molecular species of crustacean hyperglycemic hormone, Pej-SGP-I, -II, -III, -V, VI, and VII, displayed significant inhibitory effects on vg expression with almost the same efficacies, whereas Pej-SGP-IV (known as molt-inhibiting hormone) did not. Two chromatophorotropic peptides, red pigment-concentrating hormone and pigment-dispersing hormone, which were also present in the sinus glands, did not have a clear effect on the gene expression levels in this incubation system. These results suggest that the six crustacean hyperglycemic hormones are potentially capable of acting as vitellogenesis-inhibiting hormones in M. japonicus.
KW - Crustacean hyperglycemic hormone
KW - Kuruma prawn
KW - Marsupenaeus japonicus
KW - Sinus gland
KW - Vitellogenesis
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U2 - 10.1007/s12562-012-0566-5
DO - 10.1007/s12562-012-0566-5
M3 - Article
AN - SCOPUS:84872268940
SN - 0919-9268
VL - 79
SP - 33
EP - 38
JO - Fisheries Science
JF - Fisheries Science
IS - 1
ER -