The ex vivo effects of eyestalk peptides on ovarian vitellogenin gene expression in the kuruma prawn Marsupenaeus japonicus

Naoaki Tsutsui, Ayano Nagakura-Nakamura, Chiaki Nagai, Tsuyoshi Ohira, Marcy N. Wilder, Hiromichi Nagasawa

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Seven major peptides belonging to the crustacean hyperglycemic hormone family were purified from the sinus gland located in the eyestalk of the kuruma prawn Marsupenaeus japonicus, and their effects on vitellogenin gene expression were examined using the ex vivo ovary incubation system. Six molecular species of crustacean hyperglycemic hormone, Pej-SGP-I, -II, -III, -V, VI, and VII, displayed significant inhibitory effects on vg expression with almost the same efficacies, whereas Pej-SGP-IV (known as molt-inhibiting hormone) did not. Two chromatophorotropic peptides, red pigment-concentrating hormone and pigment-dispersing hormone, which were also present in the sinus glands, did not have a clear effect on the gene expression levels in this incubation system. These results suggest that the six crustacean hyperglycemic hormones are potentially capable of acting as vitellogenesis-inhibiting hormones in M. japonicus.

Original languageEnglish
Pages (from-to)33-38
Number of pages6
JournalFisheries Science
Volume79
Issue number1
DOIs
Publication statusPublished - 2013

Fingerprint

Marsupenaeus japonicus
vitellogenin
peptide
gene expression
hormone
hormones
peptides
Crustacea
crustacean
sinuses
red pigment-concentrating hormone
pigment
incubation
vitellogenesis
molt
molting
effect
pigments

Keywords

  • Crustacean hyperglycemic hormone
  • Kuruma prawn
  • Marsupenaeus japonicus
  • Sinus gland
  • Vitellogenesis

ASJC Scopus subject areas

  • Aquatic Science

Cite this

The ex vivo effects of eyestalk peptides on ovarian vitellogenin gene expression in the kuruma prawn Marsupenaeus japonicus. / Tsutsui, Naoaki; Nagakura-Nakamura, Ayano; Nagai, Chiaki; Ohira, Tsuyoshi; Wilder, Marcy N.; Nagasawa, Hiromichi.

In: Fisheries Science, Vol. 79, No. 1, 2013, p. 33-38.

Research output: Contribution to journalArticle

Tsutsui, Naoaki ; Nagakura-Nakamura, Ayano ; Nagai, Chiaki ; Ohira, Tsuyoshi ; Wilder, Marcy N. ; Nagasawa, Hiromichi. / The ex vivo effects of eyestalk peptides on ovarian vitellogenin gene expression in the kuruma prawn Marsupenaeus japonicus. In: Fisheries Science. 2013 ; Vol. 79, No. 1. pp. 33-38.
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