The Essential Role of Fibroblasts in Esophageal Squamous Cell Carcinoma-Induced Angiogenesis

Kazuhiro Noma, Keiran S.M. Smalley, Mercedes Lioni, Yoshio Naomoto, Noriaki Tanaka, Wafik El-Deiry, Alastair J. King, Hiroshi Nakagawa, Meenhard Herlyn

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78 Citations (Scopus)


Background & Aims: Esophageal squamous cell carcinoma (ESCC) is known to be a highly angiogenic tumor. Here, we investigated the role of the stromal fibroblasts in the ESCC-induced angiogenic response using a novel 3-dimensional model. Methods: A novel assay was developed where cocultures of ESCC and esophageal fibroblasts induced human microvascular endothelial cell (HMVEC) vascular network formation in a 3-dimensional collagen gel. Biochemical studies showed that the ESCC-induced activation of the fibroblasts was required to induce vascular network formation via a transforming growth factor (TGF)-β and vascular endothelial growth factor (VEGF)-dependent pathway. Results: Conditioned media from a panel of 4 ESCC lines transdifferentiated normal esophageal fibroblasts into myofibroblasts via TGF-β signaling. The presence of fibroblasts was essential for efficient HMVEC network formation, and the addition of ESCC cells to these cultures greatly enhanced the angiogenic process. The role of TGF-β in this process was shown by the complete inhibition of network formation following TGF-β inhibitor treatment. Finally, we showed that ESCC-derived TGF-β regulates angiogenesis through the release of VEGF from the fibroblasts and that the VEGF release was blocked following TGF-β inhibition. Conclusions: This study shows the essential role of fibroblasts in the ESCC angiogenic-induced response and suggests that the pharmacologic targeting of the TGF-β signaling axis could be of therapeutic benefit in this deadly disease.

Original languageEnglish
Pages (from-to)1981-1993
Number of pages13
Issue number7
Publication statusPublished - Jun 2008

ASJC Scopus subject areas

  • Hepatology
  • Gastroenterology

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    Noma, K., Smalley, K. S. M., Lioni, M., Naomoto, Y., Tanaka, N., El-Deiry, W., King, A. J., Nakagawa, H., & Herlyn, M. (2008). The Essential Role of Fibroblasts in Esophageal Squamous Cell Carcinoma-Induced Angiogenesis. Gastroenterology, 134(7), 1981-1993.