Abstract
Plant cells develop various endoplasmic reticulum (ER)-derived structures with specific functions. The ER body, a novel ER-derived compartment in Arabidopsis, is a spindle-shaped structure (∼10 μm long and ∼1 μm wide) that is surrounded by ribosomes. Similar structures were found in many Brassicaceae plants in the 1960s and 1970s, but their main components and biological functions have remained unknown. ER bodies can be visualized in transgenic Arabidopsis expressing the green fluorescent protein with an ER-retention signal. A large number of ER bodies are observed in cotyledons, hypocotyls and roots of seedlings, but very few are observed in rosette leaves. Recently nai1, a mutant that does not develop ER bodies in whole seedlings, was isolated. Analysis of the nai1 mutant reveals that a β-glucosidase, called PYK10, is the main component of ER bodies. The putative biological function of PYK10 and the inducibility of ER bodies in rosette leaves by wound stress suggest that the ER body functions in the defense against herbivores.
| Original language | English |
|---|---|
| Pages (from-to) | 661-666 |
| Number of pages | 6 |
| Journal | Plant and Cell Physiology |
| Volume | 44 |
| Issue number | 7 |
| DOIs | |
| Publication status | Published - Jul 1 2003 |
| Externally published | Yes |
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Keywords
- β-Glucosidase
- Arabidopsis thaliana
- Endoplasmic reticulum
- ER body
- GFP
- PYK10
ASJC Scopus subject areas
- Plant Science
- Physiology
- Cell Biology
Cite this
The ER body, a novel endoplasmic reticulum-derived structure in Arabidopsis. / Matsushima, Ryo; Hayashi, Yasuko; Yamada, Kenji; Shimada, Tomoo; Nishimura, Mikio; Hara-Nishimura, Ikuko.
In: Plant and Cell Physiology, Vol. 44, No. 7, 01.07.2003, p. 661-666.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The ER body, a novel endoplasmic reticulum-derived structure in Arabidopsis
AU - Matsushima, Ryo
AU - Hayashi, Yasuko
AU - Yamada, Kenji
AU - Shimada, Tomoo
AU - Nishimura, Mikio
AU - Hara-Nishimura, Ikuko
PY - 2003/7/1
Y1 - 2003/7/1
N2 - Plant cells develop various endoplasmic reticulum (ER)-derived structures with specific functions. The ER body, a novel ER-derived compartment in Arabidopsis, is a spindle-shaped structure (∼10 μm long and ∼1 μm wide) that is surrounded by ribosomes. Similar structures were found in many Brassicaceae plants in the 1960s and 1970s, but their main components and biological functions have remained unknown. ER bodies can be visualized in transgenic Arabidopsis expressing the green fluorescent protein with an ER-retention signal. A large number of ER bodies are observed in cotyledons, hypocotyls and roots of seedlings, but very few are observed in rosette leaves. Recently nai1, a mutant that does not develop ER bodies in whole seedlings, was isolated. Analysis of the nai1 mutant reveals that a β-glucosidase, called PYK10, is the main component of ER bodies. The putative biological function of PYK10 and the inducibility of ER bodies in rosette leaves by wound stress suggest that the ER body functions in the defense against herbivores.
AB - Plant cells develop various endoplasmic reticulum (ER)-derived structures with specific functions. The ER body, a novel ER-derived compartment in Arabidopsis, is a spindle-shaped structure (∼10 μm long and ∼1 μm wide) that is surrounded by ribosomes. Similar structures were found in many Brassicaceae plants in the 1960s and 1970s, but their main components and biological functions have remained unknown. ER bodies can be visualized in transgenic Arabidopsis expressing the green fluorescent protein with an ER-retention signal. A large number of ER bodies are observed in cotyledons, hypocotyls and roots of seedlings, but very few are observed in rosette leaves. Recently nai1, a mutant that does not develop ER bodies in whole seedlings, was isolated. Analysis of the nai1 mutant reveals that a β-glucosidase, called PYK10, is the main component of ER bodies. The putative biological function of PYK10 and the inducibility of ER bodies in rosette leaves by wound stress suggest that the ER body functions in the defense against herbivores.
KW - β-Glucosidase
KW - Arabidopsis thaliana
KW - Endoplasmic reticulum
KW - ER body
KW - GFP
KW - PYK10
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UR - http://www.scopus.com/inward/citedby.url?scp=0041696481&partnerID=8YFLogxK
U2 - 10.1093/pcp/pcg089
DO - 10.1093/pcp/pcg089
M3 - Article
C2 - 12881493
AN - SCOPUS:0041696481
VL - 44
SP - 661
EP - 666
JO - Plant and Cell Physiology
JF - Plant and Cell Physiology
SN - 0032-0781
IS - 7
ER -