The ER body, a novel endoplasmic reticulum-derived structure in Arabidopsis

Ryo Matsushima, Yasuko Hayashi, Kenji Yamada, Tomoo Shimada, Mikio Nishimura, Ikuko Hara-Nishimura

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

Plant cells develop various endoplasmic reticulum (ER)-derived structures with specific functions. The ER body, a novel ER-derived compartment in Arabidopsis, is a spindle-shaped structure (∼10 μm long and ∼1 μm wide) that is surrounded by ribosomes. Similar structures were found in many Brassicaceae plants in the 1960s and 1970s, but their main components and biological functions have remained unknown. ER bodies can be visualized in transgenic Arabidopsis expressing the green fluorescent protein with an ER-retention signal. A large number of ER bodies are observed in cotyledons, hypocotyls and roots of seedlings, but very few are observed in rosette leaves. Recently nai1, a mutant that does not develop ER bodies in whole seedlings, was isolated. Analysis of the nai1 mutant reveals that a β-glucosidase, called PYK10, is the main component of ER bodies. The putative biological function of PYK10 and the inducibility of ER bodies in rosette leaves by wound stress suggest that the ER body functions in the defense against herbivores.

Original languageEnglish
Pages (from-to)661-666
Number of pages6
JournalPlant and Cell Physiology
Volume44
Issue number7
DOIs
Publication statusPublished - Jul 1 2003
Externally publishedYes

Fingerprint

Arabidopsis
Endoplasmic Reticulum
endoplasmic reticulum
Seedlings
Glucosidases
Brassicaceae
Hypocotyl
Herbivory
Endoplasmic Reticulum Stress
Cotyledon
mutants
glucosidases
Plant Cells
seedlings
Green Fluorescent Proteins
Ribosomes
plant damage
ribosomes
green fluorescent protein
hypocotyls

Keywords

  • β-Glucosidase
  • Arabidopsis thaliana
  • Endoplasmic reticulum
  • ER body
  • GFP
  • PYK10

ASJC Scopus subject areas

  • Plant Science
  • Physiology
  • Cell Biology

Cite this

Matsushima, R., Hayashi, Y., Yamada, K., Shimada, T., Nishimura, M., & Hara-Nishimura, I. (2003). The ER body, a novel endoplasmic reticulum-derived structure in Arabidopsis. Plant and Cell Physiology, 44(7), 661-666. https://doi.org/10.1093/pcp/pcg089

The ER body, a novel endoplasmic reticulum-derived structure in Arabidopsis. / Matsushima, Ryo; Hayashi, Yasuko; Yamada, Kenji; Shimada, Tomoo; Nishimura, Mikio; Hara-Nishimura, Ikuko.

In: Plant and Cell Physiology, Vol. 44, No. 7, 01.07.2003, p. 661-666.

Research output: Contribution to journalArticle

Matsushima, R, Hayashi, Y, Yamada, K, Shimada, T, Nishimura, M & Hara-Nishimura, I 2003, 'The ER body, a novel endoplasmic reticulum-derived structure in Arabidopsis', Plant and Cell Physiology, vol. 44, no. 7, pp. 661-666. https://doi.org/10.1093/pcp/pcg089
Matsushima, Ryo ; Hayashi, Yasuko ; Yamada, Kenji ; Shimada, Tomoo ; Nishimura, Mikio ; Hara-Nishimura, Ikuko. / The ER body, a novel endoplasmic reticulum-derived structure in Arabidopsis. In: Plant and Cell Physiology. 2003 ; Vol. 44, No. 7. pp. 661-666.
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