The EP2 receptor is the predominant prostanoid receptor in the human ciliary muscle

Toshihiko Matsuo, Max S. Cynader

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Prostaglandins can reduce intraocular pressure by increasing uveoscleral outflow. We have previously demonstrated that the human ciliary muscle was a zone of concentration for binding sites (receptors) for prostaglandin F 2α and for prostaglandin E2. Here, we try to elucidate the types of prostanoid receptors in the ciliary muscle using competitive ligand binding studies in human eye sections and computer assisted autoradiographic densitometry. Saturation binding curves showed that the human ciliary muscle had a large number of binding sites with a high affinity for prostaglandin E2compared with prostaglandin D2 and F 2,. The binding of tritiated prostaglandin E2 and F 2α in the ciliary muscle was displaced most effectively by prostaglandin E2 and 11- deoxy prostaglandin E1 (a selective EP2 prostanoid receptor agonist), whereas the binding of prostaglandin D2 was displaced most effectively by prostaglandin E2 and D2. These results indicate that the dominant prostanoid receptor in the human ciliary muscle is the EP2 subclass and that there is also a small number of DP receptors.

Original languageEnglish
Pages (from-to)110-114
Number of pages5
JournalBritish Journal of Ophthalmology
Volume77
Issue number2
DOIs
Publication statusPublished - 1993
Externally publishedYes

Fingerprint

Prostaglandins
Dinoprostone
Prostaglandin D2
Prostaglandins F
Muscles
Receptors, Prostaglandin E, EP2 Subtype
Binding Sites
Competitive Binding
Densitometry
Alprostadil
Intraocular Pressure
Ligands

ASJC Scopus subject areas

  • Critical Care and Intensive Care Medicine
  • Emergency Medicine
  • Ophthalmology

Cite this

The EP2 receptor is the predominant prostanoid receptor in the human ciliary muscle. / Matsuo, Toshihiko; Cynader, Max S.

In: British Journal of Ophthalmology, Vol. 77, No. 2, 1993, p. 110-114.

Research output: Contribution to journalArticle

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