The enigmatic genome of Chara australis virus

Adrian J. Gibbs, Marjo Torronen, Anne M. Mackenzie, Jeffery T. Wood, John S. Armstrong, Hideki Kondo, Tetsuo Tamada, Paul L. Keese

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Most of the genomic sequence of Chara australis virus (CAV), previously called Chara corallina virus, has been determined. It is a ssRNA molecule of 9065 nt with at least four ORFs. At its 59 end is an ORF encoding a protein of 227 kDa, distantly homologous to the multifunctional replicases of benyviruses and rubiviruses. Next is an ORF encoding a protein of 44 kDa, homologous to the helicases of pestiviruses. The third ORF encodes an unmatched protein of 38 kDa that is probably a movement protein. The fourth and 39-terminal ORF encodes a protein of 17.7 kDa homologous to the coat proteins of tobamoviruses. The short methyltransferase region of the CAV replicase matches only the C-terminal motif of benyvirus methyltransferases. This and other clues indicate that approximately 11% and 2% of the 59 and 39 termini of the complete CAV genome, respectively, are missing from the sequence. The aligned amino acid sequences of the CAV proteins and their nearest homologues contain many gaps but relationships inferred from them were little affected by removal of these gaps. Sequence comparisons show that three of the CAV genes may have diverged from the most closely related genes of other viruses 250-450 million years ago, and the sister relationship between the genes of CAV and those of benyviruses and tobamoviruses, mirroring the ancient sister relationship between charophytes (i.e. the algal host of CAV) and embryophytes (i.e. the plant hosts of tobamoviruses and benyviruses), is congruent with this possibility.

Original languageEnglish
Pages (from-to)2679-2690
Number of pages12
JournalJournal of General Virology
Volume92
Issue number11
DOIs
Publication statusPublished - Nov 2011

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Chara
Genome
Viruses
Tobamovirus
Open Reading Frames
Proteins
Methyltransferases
Rubivirus
Charophyceae
Pestivirus
Embryophyta
Genes
Capsid Proteins
Amino Acid Sequence

ASJC Scopus subject areas

  • Virology

Cite this

Gibbs, A. J., Torronen, M., Mackenzie, A. M., Wood, J. T., Armstrong, J. S., Kondo, H., ... Keese, P. L. (2011). The enigmatic genome of Chara australis virus. Journal of General Virology, 92(11), 2679-2690. https://doi.org/10.1099/vir.0.033852-0

The enigmatic genome of Chara australis virus. / Gibbs, Adrian J.; Torronen, Marjo; Mackenzie, Anne M.; Wood, Jeffery T.; Armstrong, John S.; Kondo, Hideki; Tamada, Tetsuo; Keese, Paul L.

In: Journal of General Virology, Vol. 92, No. 11, 11.2011, p. 2679-2690.

Research output: Contribution to journalArticle

Gibbs, AJ, Torronen, M, Mackenzie, AM, Wood, JT, Armstrong, JS, Kondo, H, Tamada, T & Keese, PL 2011, 'The enigmatic genome of Chara australis virus', Journal of General Virology, vol. 92, no. 11, pp. 2679-2690. https://doi.org/10.1099/vir.0.033852-0
Gibbs AJ, Torronen M, Mackenzie AM, Wood JT, Armstrong JS, Kondo H et al. The enigmatic genome of Chara australis virus. Journal of General Virology. 2011 Nov;92(11):2679-2690. https://doi.org/10.1099/vir.0.033852-0
Gibbs, Adrian J. ; Torronen, Marjo ; Mackenzie, Anne M. ; Wood, Jeffery T. ; Armstrong, John S. ; Kondo, Hideki ; Tamada, Tetsuo ; Keese, Paul L. / The enigmatic genome of Chara australis virus. In: Journal of General Virology. 2011 ; Vol. 92, No. 11. pp. 2679-2690.
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