The engraftment and differentiation of transplanted bone marrow-derived cells in the olfactory bulb after methimazole administration

Yohei Noda, Kazunori Nishizaki, Junko Yoshinobu, Yorihisa Orita, Hidetsugu Tsujigiwa, Masao Yamada

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3 Citations (Scopus)


Conclusion: Bone marrow-derived cells can be engrafted in the olfactory bulb and a few cells can differentiate into mitral/tufted cells in the olfactory bulb. Objectives: To investigate whether bone marrow-derived cells can be engrafted into the olfactory bulb and differentiate into neurons and glial cells after methimazole administration. Methods: Bone marrow of GFP (green fluorescence protein) mice was transplanted into lethally irradiated recipient mice. Immunostaining was performed to confirm the cell types of bone marrow-derived cells expressing GFP. Results: GFP-positive cells were observed in the olfactory bulb at 2 days after methimazole administration. The number of dendritic GFP-positive cells increased up to 30 days after methimazole administration and then decreased. Double immunostaining for GFP and Iba1 or TBX21 showed that a large population of the GFP-positive cells had characteristics of microglia/macrophages and a few cells had characteristics of mitral/tufted cells.

Original languageEnglish
Pages (from-to)951-956
Number of pages6
JournalActa Oto-Laryngologica
Issue number9
Publication statusPublished - Sep 1 2013



  • Microglia
  • Mitral cells
  • Olfaction
  • Regeneration
  • Transplantation

ASJC Scopus subject areas

  • Otorhinolaryngology

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