Abstract
Conclusion: Bone marrow-derived cells can be engrafted in the olfactory bulb and a few cells can differentiate into mitral/tufted cells in the olfactory bulb. Objectives: To investigate whether bone marrow-derived cells can be engrafted into the olfactory bulb and differentiate into neurons and glial cells after methimazole administration. Methods: Bone marrow of GFP (green fluorescence protein) mice was transplanted into lethally irradiated recipient mice. Immunostaining was performed to confirm the cell types of bone marrow-derived cells expressing GFP. Results: GFP-positive cells were observed in the olfactory bulb at 2 days after methimazole administration. The number of dendritic GFP-positive cells increased up to 30 days after methimazole administration and then decreased. Double immunostaining for GFP and Iba1 or TBX21 showed that a large population of the GFP-positive cells had characteristics of microglia/macrophages and a few cells had characteristics of mitral/tufted cells.
| Original language | English |
|---|---|
| Pages (from-to) | 951-956 |
| Number of pages | 6 |
| Journal | Acta Oto-Laryngologica |
| Volume | 133 |
| Issue number | 9 |
| DOIs | |
| Publication status | Published - Sep 2013 |
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Keywords
- Microglia
- Mitral cells
- Olfaction
- Regeneration
- Transplantation
ASJC Scopus subject areas
- Otorhinolaryngology
Cite this
The engraftment and differentiation of transplanted bone marrow-derived cells in the olfactory bulb after methimazole administration. / Noda, Yohei; Nishizaki, Kazunori; Yoshinobu, Junko; Orita, Yorihisa; Tsujigiwa, Hidetsugu; Yamada, Masao.
In: Acta Oto-Laryngologica, Vol. 133, No. 9, 09.2013, p. 951-956.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The engraftment and differentiation of transplanted bone marrow-derived cells in the olfactory bulb after methimazole administration
AU - Noda, Yohei
AU - Nishizaki, Kazunori
AU - Yoshinobu, Junko
AU - Orita, Yorihisa
AU - Tsujigiwa, Hidetsugu
AU - Yamada, Masao
PY - 2013/9
Y1 - 2013/9
N2 - Conclusion: Bone marrow-derived cells can be engrafted in the olfactory bulb and a few cells can differentiate into mitral/tufted cells in the olfactory bulb. Objectives: To investigate whether bone marrow-derived cells can be engrafted into the olfactory bulb and differentiate into neurons and glial cells after methimazole administration. Methods: Bone marrow of GFP (green fluorescence protein) mice was transplanted into lethally irradiated recipient mice. Immunostaining was performed to confirm the cell types of bone marrow-derived cells expressing GFP. Results: GFP-positive cells were observed in the olfactory bulb at 2 days after methimazole administration. The number of dendritic GFP-positive cells increased up to 30 days after methimazole administration and then decreased. Double immunostaining for GFP and Iba1 or TBX21 showed that a large population of the GFP-positive cells had characteristics of microglia/macrophages and a few cells had characteristics of mitral/tufted cells.
AB - Conclusion: Bone marrow-derived cells can be engrafted in the olfactory bulb and a few cells can differentiate into mitral/tufted cells in the olfactory bulb. Objectives: To investigate whether bone marrow-derived cells can be engrafted into the olfactory bulb and differentiate into neurons and glial cells after methimazole administration. Methods: Bone marrow of GFP (green fluorescence protein) mice was transplanted into lethally irradiated recipient mice. Immunostaining was performed to confirm the cell types of bone marrow-derived cells expressing GFP. Results: GFP-positive cells were observed in the olfactory bulb at 2 days after methimazole administration. The number of dendritic GFP-positive cells increased up to 30 days after methimazole administration and then decreased. Double immunostaining for GFP and Iba1 or TBX21 showed that a large population of the GFP-positive cells had characteristics of microglia/macrophages and a few cells had characteristics of mitral/tufted cells.
KW - Microglia
KW - Mitral cells
KW - Olfaction
KW - Regeneration
KW - Transplantation
UR - http://www.scopus.com/inward/record.url?scp=84882389820&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84882389820&partnerID=8YFLogxK
U2 - 10.3109/00016489.2013.803153
DO - 10.3109/00016489.2013.803153
M3 - Article
C2 - 23822107
AN - SCOPUS:84882389820
VL - 133
SP - 951
EP - 956
JO - Acta Oto-Laryngologica
JF - Acta Oto-Laryngologica
SN - 0001-6489
IS - 9
ER -