Abstract
Conclusion: Bone marrow-derived cells can be engrafted in the olfactory bulb and a few cells can differentiate into mitral/tufted cells in the olfactory bulb. Objectives: To investigate whether bone marrow-derived cells can be engrafted into the olfactory bulb and differentiate into neurons and glial cells after methimazole administration. Methods: Bone marrow of GFP (green fluorescence protein) mice was transplanted into lethally irradiated recipient mice. Immunostaining was performed to confirm the cell types of bone marrow-derived cells expressing GFP. Results: GFP-positive cells were observed in the olfactory bulb at 2 days after methimazole administration. The number of dendritic GFP-positive cells increased up to 30 days after methimazole administration and then decreased. Double immunostaining for GFP and Iba1 or TBX21 showed that a large population of the GFP-positive cells had characteristics of microglia/macrophages and a few cells had characteristics of mitral/tufted cells.
Original language | English |
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Pages (from-to) | 951-956 |
Number of pages | 6 |
Journal | Acta Oto-Laryngologica |
Volume | 133 |
Issue number | 9 |
DOIs | |
Publication status | Published - Sep 2013 |
Externally published | Yes |
Keywords
- Microglia
- Mitral cells
- Olfaction
- Regeneration
- Transplantation
ASJC Scopus subject areas
- Otorhinolaryngology