The effect of Wolbachia on diapause, fecundity, and clock gene expression in Trichogramma brassicae (Hymenoptera: Trichogrammatidae)

Somayeh Rahimi-Kaldeh, Ahmad Ashouri, Alireza Bandani, Kenji Tomioka

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The short day lengths of late summer in moderate regions are used to induce diapause in various insects. Many studies have shown the maternal effect of photoperiod on diapause induction of Trichogramma wasps, but there is no study to show the relationship between photoperiodic regimes and clock genes in these useful biological control agents. Here, we investigated the role of photoperiods on diapause, fecundity, and clock gene expression (clk, cyc, cry2, per, and timeout) in asexual and sexual Trichogramma brassicae as a model insect to find any differences between two strains. Asexual strain was infected by Wolbachia, an endosymbiont bacterium. The diapause percentage was significantly higher under short days (8 h in sexual and 12 h in the asexual T. brassicae), although the diapause percentage of the sexual strain was significantly higher than the asexual one in all the photoperiods. The ANOVA revealed no significant changes between different photoperiods in the clock gene expression in the sexual strain but significant photoperiodic changes in clk, cyc, and timeout in the asexual strain. Our results showed that the mRNA levels of clock genes of asexual T. brassicae were significantly lower than those of sexual strain. The fecundity was significantly higher in the asexual strain. These results suggest that Wolbachia infection makes disturbance on the clock gene expression which consequently reduces the percentage of diapause but increases the fecundity in asexual T. brassicae.

Original languageEnglish
Pages (from-to)401-410
Number of pages10
JournalDevelopment Genes and Evolution
Volume227
Issue number6
DOIs
Publication statusPublished - Nov 1 2017

Fingerprint

Wolbachia
Hymenoptera
Photoperiod
Fertility
Gene Expression
Insects
Biological Control Agents
Wasps
Genes
Analysis of Variance
Bacteria
Messenger RNA
Infection

Keywords

  • Clock genes
  • Diapause
  • Fecundity
  • Photoperiod
  • Trichogramma brassicae
  • Wolbachia

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology

Cite this

The effect of Wolbachia on diapause, fecundity, and clock gene expression in Trichogramma brassicae (Hymenoptera : Trichogrammatidae). / Rahimi-Kaldeh, Somayeh; Ashouri, Ahmad; Bandani, Alireza; Tomioka, Kenji.

In: Development Genes and Evolution, Vol. 227, No. 6, 01.11.2017, p. 401-410.

Research output: Contribution to journalArticle

@article{ee85b10c8f674a04b67a09f6f5aedefe,
title = "The effect of Wolbachia on diapause, fecundity, and clock gene expression in Trichogramma brassicae (Hymenoptera: Trichogrammatidae)",
abstract = "The short day lengths of late summer in moderate regions are used to induce diapause in various insects. Many studies have shown the maternal effect of photoperiod on diapause induction of Trichogramma wasps, but there is no study to show the relationship between photoperiodic regimes and clock genes in these useful biological control agents. Here, we investigated the role of photoperiods on diapause, fecundity, and clock gene expression (clk, cyc, cry2, per, and timeout) in asexual and sexual Trichogramma brassicae as a model insect to find any differences between two strains. Asexual strain was infected by Wolbachia, an endosymbiont bacterium. The diapause percentage was significantly higher under short days (8 h in sexual and 12 h in the asexual T. brassicae), although the diapause percentage of the sexual strain was significantly higher than the asexual one in all the photoperiods. The ANOVA revealed no significant changes between different photoperiods in the clock gene expression in the sexual strain but significant photoperiodic changes in clk, cyc, and timeout in the asexual strain. Our results showed that the mRNA levels of clock genes of asexual T. brassicae were significantly lower than those of sexual strain. The fecundity was significantly higher in the asexual strain. These results suggest that Wolbachia infection makes disturbance on the clock gene expression which consequently reduces the percentage of diapause but increases the fecundity in asexual T. brassicae.",
keywords = "Clock genes, Diapause, Fecundity, Photoperiod, Trichogramma brassicae, Wolbachia",
author = "Somayeh Rahimi-Kaldeh and Ahmad Ashouri and Alireza Bandani and Kenji Tomioka",
year = "2017",
month = "11",
day = "1",
doi = "10.1007/s00427-017-0597-0",
language = "English",
volume = "227",
pages = "401--410",
journal = "Development Genes and Evolution",
issn = "0949-944X",
publisher = "Springer Verlag",
number = "6",

}

TY - JOUR

T1 - The effect of Wolbachia on diapause, fecundity, and clock gene expression in Trichogramma brassicae (Hymenoptera

T2 - Trichogrammatidae)

AU - Rahimi-Kaldeh, Somayeh

AU - Ashouri, Ahmad

AU - Bandani, Alireza

AU - Tomioka, Kenji

PY - 2017/11/1

Y1 - 2017/11/1

N2 - The short day lengths of late summer in moderate regions are used to induce diapause in various insects. Many studies have shown the maternal effect of photoperiod on diapause induction of Trichogramma wasps, but there is no study to show the relationship between photoperiodic regimes and clock genes in these useful biological control agents. Here, we investigated the role of photoperiods on diapause, fecundity, and clock gene expression (clk, cyc, cry2, per, and timeout) in asexual and sexual Trichogramma brassicae as a model insect to find any differences between two strains. Asexual strain was infected by Wolbachia, an endosymbiont bacterium. The diapause percentage was significantly higher under short days (8 h in sexual and 12 h in the asexual T. brassicae), although the diapause percentage of the sexual strain was significantly higher than the asexual one in all the photoperiods. The ANOVA revealed no significant changes between different photoperiods in the clock gene expression in the sexual strain but significant photoperiodic changes in clk, cyc, and timeout in the asexual strain. Our results showed that the mRNA levels of clock genes of asexual T. brassicae were significantly lower than those of sexual strain. The fecundity was significantly higher in the asexual strain. These results suggest that Wolbachia infection makes disturbance on the clock gene expression which consequently reduces the percentage of diapause but increases the fecundity in asexual T. brassicae.

AB - The short day lengths of late summer in moderate regions are used to induce diapause in various insects. Many studies have shown the maternal effect of photoperiod on diapause induction of Trichogramma wasps, but there is no study to show the relationship between photoperiodic regimes and clock genes in these useful biological control agents. Here, we investigated the role of photoperiods on diapause, fecundity, and clock gene expression (clk, cyc, cry2, per, and timeout) in asexual and sexual Trichogramma brassicae as a model insect to find any differences between two strains. Asexual strain was infected by Wolbachia, an endosymbiont bacterium. The diapause percentage was significantly higher under short days (8 h in sexual and 12 h in the asexual T. brassicae), although the diapause percentage of the sexual strain was significantly higher than the asexual one in all the photoperiods. The ANOVA revealed no significant changes between different photoperiods in the clock gene expression in the sexual strain but significant photoperiodic changes in clk, cyc, and timeout in the asexual strain. Our results showed that the mRNA levels of clock genes of asexual T. brassicae were significantly lower than those of sexual strain. The fecundity was significantly higher in the asexual strain. These results suggest that Wolbachia infection makes disturbance on the clock gene expression which consequently reduces the percentage of diapause but increases the fecundity in asexual T. brassicae.

KW - Clock genes

KW - Diapause

KW - Fecundity

KW - Photoperiod

KW - Trichogramma brassicae

KW - Wolbachia

UR - http://www.scopus.com/inward/record.url?scp=85035362768&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85035362768&partnerID=8YFLogxK

U2 - 10.1007/s00427-017-0597-0

DO - 10.1007/s00427-017-0597-0

M3 - Article

C2 - 29188381

AN - SCOPUS:85035362768

VL - 227

SP - 401

EP - 410

JO - Development Genes and Evolution

JF - Development Genes and Evolution

SN - 0949-944X

IS - 6

ER -