The effect of single nucleotide polymorphisms in the tumor necrosis factor-α gene on reproductive performance and immune function in dairy cattle

Yurie Kawasaki, Yuka Aoki, Fumie Magata, Akio Miyamoto, Chiho Kawashima, Takuo Hojo, Kiyoshi Okuda, Koumei Shirasuna, Takashi Shimizu

Research output: Contribution to journalArticle

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Abstract

The present study aimed to assess the effect of polymorphisms in the tumor necrosis factor α (TNF-α) promoter (A/A, A/G and G/G) and exons (T/T, T/C and C/C) on immune function and reproductive performance in dairy cows. The occurrence of the first postpartum ovulation within 3 weeks in the cows with the TNF-α promoter A/G and G/G genotypes was higher than in the A/A group. Among the different TNF-α exon genotypes, the occurrence of early first postpartum ovulation was higher in the T/C and C/C genotype groups than in the T/T group. Single nucleotide polymorphisms (SNPs) in the TNF-α gene did not affect the rate of artificial insemination (AI) or duration from parturition to next conception (days open). The apoptosis rate of polymorphonuclear leukocytes (PMNs) did not differ among the TNF-α promoter genotypes, but the PMN transmigration rate was significantly higher for the A/A and A/G genotypes than for the G/G genotype. Interleukin 8 (IL-8) mRNA expression in PMNs and peripheral blood mononuclear cells (PBMCs) before culture was significantly higher for the A/A genotype compared with the G/G genotype. There were no significant differences between the genotypes in the mRNA expression of TNF-α, IL-6, IL-1β, and toll-like receptor 4 (TLR4) in PMNs and PBMCs before and 4 h after culture. IL-8 and IL-1β production by PBMCs cultured for 4 h was significantly higher for the animals with the A/A genotype than for those with the G/G genotype. On the other hand, no significant difference was observed in IL-8 and IL-1β production by PMNs among different TNF-α genotypes. Taken together, these results suggest that SNP in the TNF-α gene affects immune function and reproductive performance in dairy cows.

Original languageEnglish
Pages (from-to)173-178
Number of pages6
JournalJournal of Reproduction and Development
Volume60
Issue number3
DOIs
Publication statusPublished - 2014

Fingerprint

tumor necrosis factors
single nucleotide polymorphism
dairy cattle
Single Nucleotide Polymorphism
reproductive performance
Tumor Necrosis Factor-alpha
Genotype
genotype
Genes
genes
interleukin-8
interleukin-1
mononuclear leukocytes
Interleukin-8
Interleukin-1
Blood Cells
promoter regions
Ovulation
Postpartum Period
exons

Keywords

  • (TNF-α)
  • Cattle
  • Immune function
  • Polymorphism
  • Reproductive performance
  • Tumor necrosis factor

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Medicine(all)

Cite this

The effect of single nucleotide polymorphisms in the tumor necrosis factor-α gene on reproductive performance and immune function in dairy cattle. / Kawasaki, Yurie; Aoki, Yuka; Magata, Fumie; Miyamoto, Akio; Kawashima, Chiho; Hojo, Takuo; Okuda, Kiyoshi; Shirasuna, Koumei; Shimizu, Takashi.

In: Journal of Reproduction and Development, Vol. 60, No. 3, 2014, p. 173-178.

Research output: Contribution to journalArticle

Kawasaki, Yurie ; Aoki, Yuka ; Magata, Fumie ; Miyamoto, Akio ; Kawashima, Chiho ; Hojo, Takuo ; Okuda, Kiyoshi ; Shirasuna, Koumei ; Shimizu, Takashi. / The effect of single nucleotide polymorphisms in the tumor necrosis factor-α gene on reproductive performance and immune function in dairy cattle. In: Journal of Reproduction and Development. 2014 ; Vol. 60, No. 3. pp. 173-178.
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AU - Miyamoto, Akio

AU - Kawashima, Chiho

AU - Hojo, Takuo

AU - Okuda, Kiyoshi

AU - Shirasuna, Koumei

AU - Shimizu, Takashi

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AB - The present study aimed to assess the effect of polymorphisms in the tumor necrosis factor α (TNF-α) promoter (A/A, A/G and G/G) and exons (T/T, T/C and C/C) on immune function and reproductive performance in dairy cows. The occurrence of the first postpartum ovulation within 3 weeks in the cows with the TNF-α promoter A/G and G/G genotypes was higher than in the A/A group. Among the different TNF-α exon genotypes, the occurrence of early first postpartum ovulation was higher in the T/C and C/C genotype groups than in the T/T group. Single nucleotide polymorphisms (SNPs) in the TNF-α gene did not affect the rate of artificial insemination (AI) or duration from parturition to next conception (days open). The apoptosis rate of polymorphonuclear leukocytes (PMNs) did not differ among the TNF-α promoter genotypes, but the PMN transmigration rate was significantly higher for the A/A and A/G genotypes than for the G/G genotype. Interleukin 8 (IL-8) mRNA expression in PMNs and peripheral blood mononuclear cells (PBMCs) before culture was significantly higher for the A/A genotype compared with the G/G genotype. There were no significant differences between the genotypes in the mRNA expression of TNF-α, IL-6, IL-1β, and toll-like receptor 4 (TLR4) in PMNs and PBMCs before and 4 h after culture. IL-8 and IL-1β production by PBMCs cultured for 4 h was significantly higher for the animals with the A/A genotype than for those with the G/G genotype. On the other hand, no significant difference was observed in IL-8 and IL-1β production by PMNs among different TNF-α genotypes. Taken together, these results suggest that SNP in the TNF-α gene affects immune function and reproductive performance in dairy cows.

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