The effect of donor treatment with hydrogen on lung allograft function in rats

Tomohiro Kawamura, Chien Sheng Huang, Ximei Peng, Kosuke Masutani, Norihisa Shigemura, Timothy R. Billiar, Meinoshin Okumura, Yoshiya Toyoda, Atsunori Nakao

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Background: Because inhaled hydrogen provides potent anti-inflammatory and antiapoptotic effects against acute lung injury, we hypothesized that treatment of organ donors with inhaled hydrogen during mechanical ventilation would decrease graft injury after lung transplantation. Methods: Orthotopic left lung transplants were performed using a fully allogeneic Lewis to Brown Norway rat model. The donors were exposed to mechanical ventilation with 98% oxygen plus 2% nitrogen or 2% hydrogen for 3 h prior to harvest, and the lung grafts underwent 4 h of cold storage in Perfadex (Vitrolife, Göteborg, Sweden). The graft function, histomorphologic changes, and inflammatory reactions were assessed. Results: The combination of mechanical ventilation and prolonged cold ischemia resulted in marked deterioration of gas exchange when the donors were ventilated with 2% nitrogen/98% oxygen, which was accompanied by upregulation of proinflammatory cytokines and proapoptotic molecules. These lung injuries were attenuated significantly by ventilation with 2% hydrogen. Inhaled hydrogen induced heme oxygenase-1, an antioxidant enzyme, in the lung grafts prior to implantation, which might contribute to protective effects afforded by hydrogen. Conclusion: Preloaded hydrogen gas during ventilation prior to organ procurement protected lung grafts effectively from ischemia/reperfusion-induced injury in a rat lung transplantation model.

Original languageEnglish
Pages (from-to)240-249
Number of pages10
JournalSurgery
Volume150
Issue number2
DOIs
Publication statusPublished - Aug 2011
Externally publishedYes

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Allografts
Hydrogen
Tissue Donors
Lung
Transplants
Artificial Respiration
Lung Transplantation
Therapeutics
Ventilation
Nitrogen
Gases
Oxygen
Cold Ischemia
Heme Oxygenase-1
Tissue and Organ Procurement
Acute Lung Injury
Lung Injury
Reperfusion Injury
Sweden
Anti-Inflammatory Agents

ASJC Scopus subject areas

  • Surgery

Cite this

Kawamura, T., Huang, C. S., Peng, X., Masutani, K., Shigemura, N., Billiar, T. R., ... Nakao, A. (2011). The effect of donor treatment with hydrogen on lung allograft function in rats. Surgery, 150(2), 240-249. https://doi.org/10.1016/j.surg.2011.05.019

The effect of donor treatment with hydrogen on lung allograft function in rats. / Kawamura, Tomohiro; Huang, Chien Sheng; Peng, Ximei; Masutani, Kosuke; Shigemura, Norihisa; Billiar, Timothy R.; Okumura, Meinoshin; Toyoda, Yoshiya; Nakao, Atsunori.

In: Surgery, Vol. 150, No. 2, 08.2011, p. 240-249.

Research output: Contribution to journalArticle

Kawamura, T, Huang, CS, Peng, X, Masutani, K, Shigemura, N, Billiar, TR, Okumura, M, Toyoda, Y & Nakao, A 2011, 'The effect of donor treatment with hydrogen on lung allograft function in rats', Surgery, vol. 150, no. 2, pp. 240-249. https://doi.org/10.1016/j.surg.2011.05.019
Kawamura T, Huang CS, Peng X, Masutani K, Shigemura N, Billiar TR et al. The effect of donor treatment with hydrogen on lung allograft function in rats. Surgery. 2011 Aug;150(2):240-249. https://doi.org/10.1016/j.surg.2011.05.019
Kawamura, Tomohiro ; Huang, Chien Sheng ; Peng, Ximei ; Masutani, Kosuke ; Shigemura, Norihisa ; Billiar, Timothy R. ; Okumura, Meinoshin ; Toyoda, Yoshiya ; Nakao, Atsunori. / The effect of donor treatment with hydrogen on lung allograft function in rats. In: Surgery. 2011 ; Vol. 150, No. 2. pp. 240-249.
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