The effect of ciprofloxacin on CD14 and toll-like receptor-4 expression on human monocytes

Goutaro Katsuno, Hideo Kohka Takahashi, Hiromi Iwagaki, Sachi Sugita, Shuji Mori, Shinya Saito, Tadashi Yoshino, Masahiro Nishibori, Noriaki Tanaka

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

CD14/toll-like receptor (TLR)-4 complex on monocytes/macrophages can bind lipopolysaccharide (LPS) and transduce the signals intracellularly. An antibacterial drug, ciprofloxacin (CIP), has been reported to modulate the inflammatory and immune responses. In the present study, we examined the effects of CIP on the LPS-induced activation of monocytes isolated from human peripheral blood mononuclear cells (PBMC). CIP suppressed the expression of CD14, TLR-4, intercellular adhesion molecule (ICAM)-1, B7.1, B7.2, and CD40 and the production of tumor necrosis factor (TNF)-α induced by LPS in monocytes. CIP induced the production of prostaglandin (PG)E2 and increased intracellular cyclic adenosine monophosphate (cAMP) levels. Cyclooxygenase (COX)-2 inhibitors, NS398 and indomethacin, reversed the effects of CIP on TNF-α production and reduced the levels of different surface antigens, whereas a protein kinase A (PKA) inhibitor, H89, did not. Therefore, CIP might regulate the TNF-α production induced by LPS by inhibiting the expression of LPS receptor complex, which seems to be mediated by COX-2 but not the cAMP/PKA pathway.

Original languageEnglish
Pages (from-to)247-253
Number of pages7
JournalShock
Volume25
Issue number3
DOIs
Publication statusPublished - Mar 2006

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Keywords

  • CD14
  • Ciprofloxacin
  • Lipopolysaccharide
  • Monocyte
  • Prostaglandin
  • Toll-like receptor-4

ASJC Scopus subject areas

  • Critical Care and Intensive Care Medicine
  • Physiology

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