The effect of an elevated cytokinin level using the ipt gene and N6-benzyladenine on single node and intact potato plant tuberization in vitro

Ivan Galis, Jiří Macas, Josef Vlasák, Miloš Ondřej, Henri A. Van Onckelen

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Two models of potato (Solanum tuberosum L.) tuberization in vitro (intact plants and single nodes) were used to study the role of cytokinins in this process. We applied hormone in two different ways. The exogenous addition of 10 mg · L-1N6-benzyladenine (BA) into the tuberization medium resulted in advanced tuber formation in intact plants, and microtubers appeared 10-20 days earlier than in the experiments in which no cytokinin was supplied. Transformation with the Agrobacterium tumefaciens ipt gene provided potato clones with endogenously elevated cytokinin levels (3-20 times higher zeatin riboside content in different clones). The onset of tuberization in intact ipt-transformed plants with low transgene expression was advanced in comparison with control material, and exogenously applied BA further promoted the tuberization process. On the contrary, tuberization was strongly inhibited in ipt-transformed nodes, and an external increase of the cytokinin level caused complete inhibition of expiant growth. In untransformed (control) nodes cytokinin application resulted in primary and secondary tuber formation, which depended on the BA concentration in cultivation media.

Original languageEnglish
Pages (from-to)143-150
Number of pages8
JournalJournal of Plant Growth Regulation
Volume14
Issue number3
DOIs
Publication statusPublished - Sep 1995
Externally publishedYes

Fingerprint

benzyladenine
cytokinins
potatoes
genes
tubers
clones
zeatin
Agrobacterium radiobacter
Solanum tuberosum
growth retardation
transgenes
hormones

ASJC Scopus subject areas

  • Plant Science
  • Agronomy and Crop Science

Cite this

The effect of an elevated cytokinin level using the ipt gene and N6-benzyladenine on single node and intact potato plant tuberization in vitro. / Galis, Ivan; Macas, Jiří; Vlasák, Josef; Ondřej, Miloš; Van Onckelen, Henri A.

In: Journal of Plant Growth Regulation, Vol. 14, No. 3, 09.1995, p. 143-150.

Research output: Contribution to journalArticle

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